ID A0A8H6M735_9AGAR Unreviewed; 576 AA.
AC A0A8H6M735;
DT 19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2022, sequence version 1.
DT 28-JAN-2026, entry version 11.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Name=NCS2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Synonyms=CTU2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN ORFNames=DFP72DRAFT_1032640 {ECO:0000313|EMBL:KAF6757295.1};
OS Ephemerocybe angulata.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Agaricineae; Psathyrellaceae; Ephemerocybe.
OX NCBI_TaxID=980116 {ECO:0000313|EMBL:KAF6757295.1, ECO:0000313|Proteomes:UP000521943};
RN [1] {ECO:0000313|EMBL:KAF6757295.1, ECO:0000313|Proteomes:UP000521943}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 144469 {ECO:0000313|EMBL:KAF6757295.1,
RC ECO:0000313|Proteomes:UP000521943};
RG DOE Joint Genome Institute;
RA Steindorff A.S., Carver A., Calhoun S., Stillman K., Liu H., Lipzen A.,
RA Pangilinan J., Labutti K., Bruns T.D., Grigoriev I.V.;
RT "Comparative genomics of pyrophilous fungi reveals a link between fire
RT events and developmental genes.";
RL Submitted (JUL-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with NCS6 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC Prior mcm(5) tRNA modification by the elongator complex is required for
CC 2-thiolation. May also be involved in protein urmylation.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF6757295.1}.
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DR EMBL; JACGCI010000023; KAF6757295.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A8H6M735; -.
DR OrthoDB; 25129at2759; -.
DR UniPathway; UPA00988; -.
DR Proteomes; UP000521943; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP000521943};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
FT REGION 122..148
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 524..546
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 134..144
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 528..544
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 576 AA; 61995 MW; A51A99487E978D83 CRC64;
MSTSCGNPET EQDALMTRRT KFDKTKTCVK CKESRGNIVI RHAVYCKSCF FPLISTRFRK
TLGPTINNKR GNKGANARGG RIKGLMQAPG SLVIGHSGGL GSTVLLDLVA KSYFYRSSTL
SGQLPEDSLS LGSEHPRNRA RGEGDSGDVW KGTPAVCYVE VASVIPGAKE RTEEVRAAVK
AYSDPTTGFE IDFIPLRLED AFDEGWWKTI GGTDLKQGAR DLGIDINDEA LPTLRQGSNI
PTTPLQSLHT YLSSLPTPTA IHSAISSLIR VLLLFTAASR DASHLLLGTS LTSLSMNMIS
GIAQGAGFAM VSEGLEEAWI GKNDQNISIV RPLRDIGIKE SAFWAWWSNL PVPGRDRYPS
SVSSVPLGSE TAASRVAVKQ NQKLTSIDVL TQDFIMGLEK DFPSTVSTIA RTLAKVTTKE
KGVSGGCVVC QKPAQPDVQA WKASISIRSY HDEAFVKSKH PLPPHFTSPP DAGVSAASSP
TNGLAPRLCY SCHTTFTSRS SRGIASANEV VVPLPVWVKA SLPRSSEDTT VPESKDLQTG
SNEDVAQAEL WETKKLSRGS IKDKIGAFLL DDAGDE
//
