ID A0A8H7DGZ3_9AGAR Unreviewed; 1139 AA.
AC A0A8H7DGZ3;
DT 19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2022, sequence version 1.
DT 28-JAN-2026, entry version 13.
DE RecName: Full=leucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013164};
DE EC=6.1.1.4 {ECO:0000256|ARBA:ARBA00013164};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030520};
GN ORFNames=MSAN_00393200 {ECO:0000313|EMBL:KAF7375069.1};
OS Mycena sanguinolenta.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Marasmiineae; Mycenaceae; Mycena.
OX NCBI_TaxID=230812 {ECO:0000313|EMBL:KAF7375069.1, ECO:0000313|Proteomes:UP000623467};
RN [1] {ECO:0000313|EMBL:KAF7375069.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=160909Yilan {ECO:0000313|EMBL:KAF7375069.1};
RA Tsai I.J.;
RT "Mycena genomes resolve the evolution of fungal bioluminescence.";
RL Submitted (MAY-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP +
CC diphosphate; Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00047469};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF7375069.1}.
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DR EMBL; JACAZH010000002; KAF7375069.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A8H7DGZ3; -.
DR OrthoDB; 10249672at2759; -.
DR Proteomes; UP000623467; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA deacylase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:InterPro.
DR FunFam; 3.90.740.10:FF:000001; Leucine--tRNA ligase, cytoplasmic; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 1.10.730.10; Isoleucyl-tRNA Synthetase, Domain 1; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR055416; RBD_LARS1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00395; leuS_arch; 1.
DR PANTHER; PTHR45794:SF1; LEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR45794; LEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF24810; RBD_LARS1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000623467}.
FT DOMAIN 95..163
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 250..813
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 852..974
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT DOMAIN 1008..1072
FT /note="Leucine--tRNA ligase RagD-binding"
FT /evidence="ECO:0000259|Pfam:PF24810"
SQ SEQUENCE 1139 AA; 128481 MW; 63523176ABEF1E44 CRC64;
MTHRVLDSTH RYINARRFGQ IVTIFRTLQK TLVFSRPLCN MATDTKIELV QTGKRDHLIQ
LEKKYQALWA QEKVFEVNAP DAADVRGLSP AQIHEKHPKW LGTYPYPYMN GSLHLGHAFT
SSKIEFAAGY QRMLGKRVLY PHGFHVTGLP IKASADKLIR EMEMFGSDFE NFEAVQQKLD
EEAEEKEKVD VVKDGPVDKS KGKKGKLAAK STGHVYQFQI LQSIDIPTAD IKKFADPLHW
LTHFPPIAIE DHTAFGSRID WRRKFLTTPA NPYYDAFVRW QMNKLHKLGK IKFGERYTIY
SPKDGQPCMD HDRSDGEALG PQEYTAVKME VAEWGAAAKN TIEGHVGGRK VFLVAATLRP
ETMYGQTNCF VGTAIKYGVF AMSDTEAFVC TYRAARNMAF QGISKTRGDI NQLLEIDGTK
LVGTRVKAPF AINPEVYVLP MDNVLPTKGT GVVTSVPSDS PDDCATLADL VKKPAFYSIE
PAWAAIEPVP VISTLDYGDM IAPALLKSMK IQSQKDTKQL AEAKEIAYKE GFYNGVMLVG
EFKGMKVEEA KPKVRERMIE AGLAMAYAEP EGLIISRSTD ECVVALMDQW YLDYGEAGWR
AETERLLKGM ELYQPETRHS FEKTLAWLNQ WACARTYGLG SQLPWDPHFL VESLSDSTIY
MSYYTVAQLL HDNSIDGSVP GPLGITPDQM TDEIWEYVFC DGPFPSSSPL PREKADALKH
EFNYFYPMDI RSSAKDLVPN HLTFCLYNHV ALFPEDKWPR SMRTNGHLLL NGKKMSKSTG
NSLSLREAIA KFGADSTRLA LADAGDGVED ANFDEKSANA NILRVHTLVQ WCEEMVKDEA
NLRHGPKNLY HDRVFEQEIN DLINITQSHY EAMNYKDALK YGFYEMQTCR DWYREVTSDV
GMHADLVMYW IRVAALVVSS IAPHFSEHIY STLLKSPTSI QRALWPTPSA PVDVAVIESG
AYLRGTIKTI RDAETTLVKM LSKTKGKGAK DGKATYDPKK PKSIRVYVAT TFPEWQEMCV
NVVKESYDTA TDKVDDAKVR GLLTERGLIK DKRAMPFVQD FKKRMAQFGA QTVFRRTLPF
SERDVLPEII PYLKRSMSLV DVEVYSIEDA LAKQGEDGFT KAIIESSEPG NPAVEYRNV
//
