ID A0A8H7GNL4_9ASCO Unreviewed; 398 AA.
AC A0A8H7GNL4;
DT 19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2022, sequence version 1.
DT 28-JAN-2026, entry version 15.
DE RecName: Full=Ribosomal RNA-processing protein 8 {ECO:0000256|ARBA:ARBA00076672, ECO:0000256|RuleBase:RU365074};
DE EC=2.1.1.- {ECO:0000256|RuleBase:RU365074};
GN ORFNames=HF325_004548 {ECO:0000313|EMBL:KAF8000759.1};
OS Metschnikowia pulcherrima.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Pichiomycetes;
OC Metschnikowiaceae; Metschnikowia.
OX NCBI_TaxID=27326 {ECO:0000313|EMBL:KAF8000759.1, ECO:0000313|Proteomes:UP000649328};
RN [1] {ECO:0000313|EMBL:KAF8000759.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=KIOM G15050 {ECO:0000313|EMBL:KAF8000759.1};
RA Rahmat E., Kang Y.;
RT "The Whole-Genome Sequence of Metschnikowia persimmonesis, a Novel
RT Endophytic Yeast Species Isolated from Medicinal Plant Diospyros kaki
RT Thumb.";
RL Submitted (OCT-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that
CC specifically methylates the N(1) position of adenine in helix 25.1 in
CC 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis.
CC Required for efficient pre-rRNA cleavage at site A2.
CC {ECO:0000256|RuleBase:RU365074}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604, ECO:0000256|RuleBase:RU365074}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RRP8 family.
CC {ECO:0000256|ARBA:ARBA00006301, ECO:0000256|RuleBase:RU365074}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF8000759.1}.
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DR EMBL; JACBPP010000006; KAF8000759.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A8H7GNL4; -.
DR OrthoDB; 10258825at2759; -.
DR Proteomes; UP000649328; Unassembled WGS sequence.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0016433; F:rRNA (adenine) methyltransferase activity; IEA:TreeGrafter.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IEA:TreeGrafter.
DR FunFam; 1.10.10.2150:FF:000001; Ribosomal RNA-processing protein 8; 1.
DR Gene3D; 1.10.10.2150; Ribosomal RNA-processing protein 8, N-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR007823; RRP8.
DR InterPro; IPR042036; RRP8_N.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR12787; RIBOSOMAL RNA-PROCESSING PROTEIN 8; 1.
DR PANTHER; PTHR12787:SF0; RIBOSOMAL RNA-PROCESSING PROTEIN 8; 1.
DR Pfam; PF05148; Methyltransf_8; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000256|RuleBase:RU365074};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU365074};
KW Reference proteome {ECO:0000313|Proteomes:UP000649328};
KW rRNA processing {ECO:0000256|ARBA:ARBA00022552,
KW ECO:0000256|RuleBase:RU365074};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|RuleBase:RU365074};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365074}.
FT REGION 15..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 350..379
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 22..56
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 398 AA; 45370 MW; CFDB5D226AC388F8 CRC64;
MALFDVAGWD IKAKNVQMGG LKKKEEKPKK DSKKASSDGG KLESKKRSAN EKDSEHKNKK
RNQAHSEDSN DLTDAQSEEI RAPVPDMPAN LTPLQQKMMA KLSGSRFRWI NEQLYTTTSD
NALKLVKNQP ALFDEYHQGF RSQVQLWPEN PVNVLVDQFK ARTTRPINAP GGLPGMASKK
IVLADMGCGE AQFALDINNF VREYNGKKGK KVDKSDKKKV GQQGPRTLDV DVHSFDLKKA
NERITVADIK NVPMEDESCN IAIFCLALMG TNFLDFVEEA YRILAPNGEL WIAEIKSRFS
ESTNAKAKPH GDNGVGEEFV NSLKLLGFYH KKTDNSNKMF TRFEFFKPSR EIINQRKEKL
QKRKKFVEQE TQKEELESKR NKVPEGQWLL KPCIYKRR
//
