ID A0A8H9YUI4_9PSED Unreviewed; 621 AA.
AC A0A8H9YUI4;
DT 19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2022, sequence version 1.
DT 28-JAN-2026, entry version 19.
DE RecName: Full=Selenocysteine-specific elongation factor {ECO:0000256|ARBA:ARBA00015953};
DE AltName: Full=SelB translation factor {ECO:0000256|ARBA:ARBA00031615};
GN Name=selB {ECO:0000313|EMBL:MBC3293820.1};
GN ORFNames=HU722_20065 {ECO:0000313|EMBL:MBC3293820.1};
OS Pseudomonas tritici.
OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC Pseudomonadales; Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=2745518 {ECO:0000313|EMBL:MBC3293820.1};
RN [1] {ECO:0000313|EMBL:MBC3293820.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SWRI145 {ECO:0000313|EMBL:MBC3293820.1};
RX PubMed=32752051;
RG The Broad Institute Genome Sequencing Platform;
RA Girard L., Lood C., Rokni-Zadeh H., van Noort V., Lavigne R., De Mot R.;
RT "Reliable Identification of Environmental Pseudomonas Isolates Using the
RT rpoD Gene.";
RL Microorganisms 8:E1166-E1166(2020).
CC -!- FUNCTION: Translation factor necessary for the incorporation of
CC selenocysteine into proteins. It probably replaces EF-Tu for the
CC insertion of selenocysteine directed by the UGA codon. SelB binds GTP
CC and GDP. {ECO:0000256|ARBA:ARBA00025526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:MBC3293820.1}.
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DR EMBL; JABWQF010000012; MBC3293820.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A8H9YUI4; -.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0035368; F:selenocysteine insertion sequence binding; IEA:TreeGrafter.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0000049; F:tRNA binding; IEA:TreeGrafter.
DR GO; GO:0001514; P:selenocysteine incorporation; IEA:InterPro.
DR GO; GO:0016259; P:selenocysteine metabolic process; IEA:TreeGrafter.
DR CDD; cd04171; SelB; 1.
DR CDD; cd03696; SelB_II; 1.
DR FunFam; 1.10.10.10:FF:000350; Selenocysteine-specific translation elongation factor; 1.
DR FunFam; 2.40.30.10:FF:000080; Selenocysteine-specific translation elongation factor; 1.
DR FunFam; 3.40.50.300:FF:001064; Selenocysteine-specific translation elongation factor; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 2.
DR InterPro; IPR057335; Beta-barrel_SelB.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR050543; eIF2G.
DR InterPro; IPR015190; Elong_fac_SelB-wing-hlx_typ-2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015191; SelB_WHD4.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004535; Transl_elong_SelB.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR048649; WHD_1st_3rd_SelB.
DR InterPro; IPR048931; WHD_2nd_SelB_bact.
DR NCBIfam; TIGR00475; selB; 1.
DR PANTHER; PTHR42854; EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER; 1.
DR PANTHER; PTHR42854:SF3; EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED; 1.
DR Pfam; PF25461; Beta-barrel_SelB; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF21458; WHD_1st_3rd_SelB; 1.
DR Pfam; PF09106; WHD_2nd_SelB; 1.
DR Pfam; PF21214; WHD_2nd_SelB_bact; 1.
DR Pfam; PF09107; WHD_3rd_SelB; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 3.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Elongation factor {ECO:0000313|EMBL:MBC3293820.1};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917}.
FT DOMAIN 1..170
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 621 AA; 69506 MW; C703AE3EE161BD70 CRC64;
MIIATAGHVD HGKTTLLQAI TGVNADRLPE EKKRGMTIDL GYAYWPQPDG RILGFIDVPG
HEKFLSNMLA GVGGIDHALL VVACDDGVMA QTREHLAILQ LTGNPQLTVA LTKADRVDEN
RIDEVREQLL ATLQEYGFSD APVFVTVATE GRGIDELRTH LQHLSSREQT RDHRFRLAID
RAFTVKGAGL VVTGTALSGE VKVGDSLWLT GVNKPMRVRG LHAQNQPSEQ AHAGQRIALN
IVGDAEKEQL NRGDWLLSDA PPEPSERVIV SLQAHTPLTQ WQPLHIHHAA SHVTGRVSLL
ENDLAELVFD TPLWLADNDW LVLRDISARA TLAGARVVTL NPPRRGKRKA EYLQWLAALA
RAENDKAALA VHLDRGAVNL ADFAWARQLS DEGLSRLSQQ PDFIQAGVNL LNAPVAARWQ
RKVLTTLATY HEQHQDEPGP GRERLRRMAL PMEDDALVLL LIEKMRESGI IHSHHGWLHL
PDHKAGFTPE QDAIWQKVAP LFGDEPWWVR DLAQQTGTDE QLMRQVLRHS AQQGLITAIV
KDRYYRNDRI VTFANLIREL DQEKGSTCAA DFRDRLNVGR KLAIQILEYF NRIGFTRRRS
NDHLLRDSLL FPEITSANNT N
//
