ID A0A8J5SSB1_ZIZPA Unreviewed; 490 AA.
AC A0A8J5SSB1;
DT 25-MAY-2022, integrated into UniProtKB/TrEMBL.
DT 25-MAY-2022, sequence version 1.
DT 02-APR-2025, entry version 14.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN ORFNames=GUJ93_ZPchr0004g39385 {ECO:0000313|EMBL:KAG8066055.1};
OS Zizania palustris (Northern wild rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Zizaniinae; Zizania.
OX NCBI_TaxID=103762 {ECO:0000313|EMBL:KAG8066055.1, ECO:0000313|Proteomes:UP000729402};
RN [1] {ECO:0000313|EMBL:KAG8066055.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Fresh leaf tissue {ECO:0000313|EMBL:KAG8066055.1};
RA Haas M., Kono T., Macchietto M., Millas R., McGilp L., Shao M.,
RA Duquette J., Hirsch C.N., Kimball J.;
RT "Whole Genome Assembly and Annotation of Northern Wild Rice, Zizania
RT palustris L., Supports a Whole Genome Duplication in the Zizania Genus.";
RL bioRxiv 0:0-0(2021).
RN [2] {ECO:0000313|EMBL:KAG8066055.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Fresh leaf tissue {ECO:0000313|EMBL:KAG8066055.1};
RA Kimball J.A., Haas M.W., Macchietto M., Kono T., Duquette J., Shao M.;
RL Submitted (FEB-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAG8066055.1}.
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DR EMBL; JAAALK010000285; KAG8066055.1; -; Genomic_DNA.
DR OrthoDB; 1431934at2759; -.
DR Proteomes; UP000729402; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR CDD; cd20341; BRcat_RBR_RNF14; 1.
DR CDD; cd23134; RING-HC_ITT1-like; 1.
DR CDD; cd23820; RWD_RNF14; 1.
DR FunFam; 3.10.110.10:FF:000083; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.30.40.10:FF:000358; RBR-type E3 ubiquitin transferase; 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR006575; RWD_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 1.
DR Pfam; PF05773; RWD; 1.
DR SMART; SM00647; IBR; 1.
DR SMART; SM00591; RWD; 1.
DR PROSITE; PS50908; RWD; 1.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000729402};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 149..289
FT /note="RWD"
FT /evidence="ECO:0000259|PROSITE:PS50908"
FT DOMAIN 342..490
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 346..394
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 1..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 88..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 490 AA; 53715 MW; 4E3F05CD8E4BE1CC CRC64;
MPRKSSSRAR GVIGRGPRPR PKSATQNPNP ISELPHDSGE APVGSLQGKL IQPPAHAGSG
PTAPAVIENG ASVSAAAEAL EHLDVSAAAE SAPVEVEPPL EASSSGSAAV GESFEEEALR
KLQALAGFGG EEVELTEEEA RANDQMQEDE IFALEAIFGD SVTIFNKKGG QRSFQVHLHI
EIPDGIDALA RLNYGAGSLK YGAVQDVDGS DNLVYKFRVK HLPPILLTCI MPSSYPSHQP
PLFTISVEWM DKMMISSLCD MLDTIWDDQK GMEVAYQWAQ WLQSSSLSHL GFVNEIVLSS
NSVCDHDCGD KRALSHNAAP DVLIPRMMRH NDDKCHEAFL HAIHDCMICF SQLPGIDFIK
LPCHHFFCLK CMQTYCKMHV KEGTVVKLLC PDTKCGCVVP PNILKRLLVD DEFERWEGLL
LQRTLESMSD VVYCPRCETA CLEDADNEGV CSSCLFSFCT LCRDRRHVGE KCMSPEDKLL
ILEVGGLHSI
//
