ID A0A8R7PJD3_TRIUA Unreviewed; 496 AA.
AC A0A8R7PJD3;
DT 12-OCT-2022, integrated into UniProtKB/TrEMBL.
DT 12-OCT-2022, sequence version 1.
DT 02-APR-2025, entry version 12.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
OS Triticum urartu (Red wild einkorn) (Crithodium urartu).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4572 {ECO:0000313|EnsemblPlants:TuG1812G0200004963.01.T01, ECO:0000313|Proteomes:UP000015106};
RN [1] {ECO:0000313|Proteomes:UP000015106}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. G1812 {ECO:0000313|Proteomes:UP000015106};
RX PubMed=23535596; DOI=10.1038/nature11997;
RA Ling H.Q., Zhao S., Liu D., Wang J., Sun H., Zhang C., Fan H., Li D.,
RA Dong L., Tao Y., Gao C., Wu H., Li Y., Cui Y., Guo X., Zheng S., Wang B.,
RA Yu K., Liang Q., Yang W., Lou X., Chen J., Feng M., Jian J., Zhang X.,
RA Luo G., Jiang Y., Liu J., Wang Z., Sha Y., Zhang B., Wu H., Tang D.,
RA Shen Q., Xue P., Zou S., Wang X., Liu X., Wang F., Yang Y., An X., Dong Z.,
RA Zhang K., Zhang X., Luo M.C., Dvorak J., Tong Y., Wang J., Yang H., Li Z.,
RA Wang D., Zhang A., Wang J.;
RT "Draft genome of the wheat A-genome progenitor Triticum urartu.";
RL Nature 496:87-90(2013).
RN [2] {ECO:0000313|EnsemblPlants:TuG1812G0200004963.01.T01}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. G1812
RC {ECO:0000313|EnsemblPlants:TuG1812G0200004963.01.T01};
RA Ling H., Ma B., Shi X., Liu H., Dong L., Sun H., Cao Y., Gao Q., Zheng S.,
RA Li Y., Yu Y., Du H., Qi M., Li Y., Yu H., Cui Y., Wang N., Chen C., Wu H.,
RA Zhao Y., Zhang J., Li Y., Zhou W., Zhang B., Hu W., Eijk M., Tang J.,
RA Witsenboer H., Zhao S., Li Z., Zhang A., Wang D., Liang C.;
RT "The Triticum urartu genome reveals the dynamic nature of wheat genome
RT evolution.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblPlants:TuG1812G0200004963.01.T01}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (JUN-2022) to UniProtKB.
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
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DR EnsemblPlants; TuG1812G0200004963.01.T01; TuG1812G0200004963.01.T01; TuG1812G0200004963.01.
DR Gramene; TuG1812G0200004963.01.T01; TuG1812G0200004963.01.T01; TuG1812G0200004963.01.
DR Proteomes; UP000015106; Chromosome 2.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR CDD; cd20341; BRcat_RBR_RNF14; 1.
DR CDD; cd23821; RWD_IMPACT; 1.
DR FunFam; 3.30.40.10:FF:000358; RBR-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR006575; RWD_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 2.
DR Pfam; PF05773; RWD; 1.
DR SMART; SM00647; IBR; 2.
DR SMART; SM00184; RING; 2.
DR SMART; SM00591; RWD; 1.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR SUPFAM; SSF54495; UBC-like; 1.
DR PROSITE; PS50908; RWD; 1.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000015106};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 1..146
FT /note="RWD"
FT /evidence="ECO:0000259|PROSITE:PS50908"
FT DOMAIN 199..419
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 203..251
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
SQ SEQUENCE 496 AA; 57387 MW; 0FA4ACDBCD3A7034 CRC64;
MALEAIYGDD LVEFRSKAGL RYFQICIRYD LHDDAQVCAR LSSATEKTKY GGCPDDDHDT
KEHDAGPDEF SYTCNFEYLP PLILTCLLPK SYPSKEPPYF TVTAKWMDGP DVSQLCEMLD
TIWAELPGQE VIYQWVEWIR SSSLLNLWFD GKILLGQDIQ SRRHNGDRRA ISRSISLESV
IPSMLSYSSK KRYQAFLEDL HMCKICLNQW KGSNFIKLPC QHLYCVKCME TLCKMHVKEG
TLFQLVCPDT KCNTSIPHHL LKRLLSKEEF ERWDRLALEK ALDSMADVVY CQKCVVGCVE
DEDNNAECPK CSFTFCGFRK ELWHPGKQCL TPEQKLQRRR GSGRMTEREV AQELSNIREL
YKDVRVCPKC RIAIAKSEGC NKMVCGNCGQ FFCFRCGKAI RGYSHFTNCR LFEPHDMTDL
DRQIDELQFG NQMRNQLKPV GAMVRCPRCR EMTFKDDEKY IFCMVCAASS CTMCKRIITD
RRMKSGHWGS SECYSL
//
