ID A0A8T0NWD3_PANVG Unreviewed; 915 AA.
AC A0A8T0NWD3;
DT 12-OCT-2022, integrated into UniProtKB/TrEMBL.
DT 12-OCT-2022, sequence version 1.
DT 28-JAN-2026, entry version 15.
DE RecName: Full=Probable glucan 1,3-alpha-glucosidase {ECO:0000256|ARBA:ARBA00073689};
DE AltName: Full=Glucosidase II subunit alpha {ECO:0000256|ARBA:ARBA00042895};
GN ORFNames=PVAP13_9KG621000 {ECO:0000313|EMBL:KAG2553770.1};
OS Panicum virgatum (Blackwell switchgrass).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Panicodae; Paniceae; Panicinae; Panicum;
OC Panicum sect. Hiantes.
OX NCBI_TaxID=38727 {ECO:0000313|EMBL:KAG2553770.1, ECO:0000313|Proteomes:UP000823388};
RN [1] {ECO:0000313|EMBL:KAG2553770.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AP13 {ECO:0000313|EMBL:KAG2553770.1};
RA Lovell J.T., Jenkins J., Shu S., Juenger T.E., Schmutz J.;
RT "WGS assembly of Panicum virgatum.";
RL Submitted (MAY-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Glycan metabolism; N-glycan metabolism.
CC {ECO:0000256|ARBA:ARBA00004833}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000256|ARBA:ARBA00004240}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 31 family.
CC {ECO:0000256|ARBA:ARBA00007806, ECO:0000256|RuleBase:RU361185}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAG2553770.1}.
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DR EMBL; CM029053; KAG2553770.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A8T0NWD3; -.
DR Proteomes; UP000823388; Chromosome 9K.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0090599; F:alpha-glucosidase activity; IEA:TreeGrafter.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006491; P:N-glycan processing; IEA:TreeGrafter.
DR CDD; cd06603; GH31_GANC_GANAB_alpha; 1.
DR CDD; cd14752; GH31_N; 1.
DR FunFam; 3.20.20.80:FF:000046; Glucosidase alpha, neutral C; 1.
DR FunFam; 3.20.20.80:FF:000039; Glucosidase, alpha neutral C; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR Gene3D; 2.60.40.1760; glycosyl hydrolase (family 31); 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 2.
DR InterPro; IPR033403; DUF5110.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR017853; GH.
DR InterPro; IPR030458; Glyco_hydro_31_AS.
DR InterPro; IPR048395; Glyco_hydro_31_C.
DR InterPro; IPR025887; Glyco_hydro_31_N_dom.
DR InterPro; IPR000322; Glyco_hydro_31_TIM.
DR InterPro; IPR013780; Glyco_hydro_b.
DR PANTHER; PTHR22762; ALPHA-GLUCOSIDASE; 1.
DR PANTHER; PTHR22762:SF54; BCDNA.GH04962; 1.
DR Pfam; PF17137; DUF5110; 1.
DR Pfam; PF13802; Gal_mutarotas_2; 1.
DR Pfam; PF01055; Glyco_hydro_31_2nd; 1.
DR Pfam; PF21365; Glyco_hydro_31_3rd; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
DR PROSITE; PS00129; GLYCOSYL_HYDROL_F31_1; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361185};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361185};
KW Reference proteome {ECO:0000313|Proteomes:UP000823388};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..28
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 29..915
FT /note="Probable glucan 1,3-alpha-glucosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5035763588"
FT DOMAIN 88..301
FT /note="Glycoside hydrolase family 31 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF13802"
FT DOMAIN 353..678
FT /note="Glycoside hydrolase family 31 TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01055"
FT DOMAIN 686..773
FT /note="Glycosyl hydrolase family 31 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF21365"
FT DOMAIN 792..841
FT /note="DUF5110"
FT /evidence="ECO:0000259|Pfam:PF17137"
SQ SEQUENCE 915 AA; 102929 MW; DBBEABD3C9C6F596 CRC64;
MDPPPRRPAA PLLLLLLLLA ASSPAARAWK KDEFRNCNQT PFCKRARTRA PHSLDAPLSL
AAGSLSVSPD GSISAELSHP SRPRPLVLRL SALPPHALRL QIDEDYSAAT PLHRRFHVPD
VLLPDLEART LHLPEPKTAA GVSTVALSSD LDVVVKHDPF ELTVRRAGSG DPVFSFNSHG
LFDFEPLRES KPEDETWEEH FRSHTDKRPR GPQSITFDVS FYGADFVYGL PEHGSTSLAL
RPTRGPGVEE SEPYRLFNLD VFEYLHESPF GLYGSIPFMI GHGARASSGF FWLNAAEMQI
DVLAPGWDGA TTLENGRIDT LWMAEAGVID AFFFVGSEPK DVVKQYVSVT GAPAMPQQFA
TAYHQCRWNY RDEADVDGVD AGFDEHDIPY DVLWLDIEHT DGKRYFTWDH SAFPNPEEMQ
RKIANKGRKM VTIVDPHIKR DGSFHLHQEA TEKGYYVKDA NGNDYDGWCW PGSSSYPDML
NPEIREWWAD KFSYESYKGS TPTLYIWNDM NEPSVFNGPE VTMPRDALHY GDVEHRELHN
AYGYYFHMAT ADGLLKRGEG KDRPFVLSRA FFAGTQRYGA VWTGDNSADW DHLKSSIPMV
LTLGLTGLPF SGADVGGFFG NPEPDLLVRW YQVGAFYPFF RGHAHHDTKR REPWLFGTAI
IREAIHVRYS LLPYFYTLFR EASVTGIPVM RPLWLEFPYD KETYNNGEAF MVGSSLLAQG
IYEEGQKSVS VYLPGKESWY ELRNGSPYKG SVSHKLQVLE DSIPSFQRGG TIVPRKDRFR
RSSTQMVNDP YTLVIALNSS NAAEGELYVD DGKSYDYQQG AFIHRRFVFA DSKLTSLNIA
PDNLGKKKFT SDCVVERIII LGLRSGAKKA IIEPGNLEVE IELGPVSLRS GSSPVAPTIR
RPNVRIADDW RIRIA
//
