ID A0A8T0RE93_PANVG Unreviewed; 808 AA.
AC A0A8T0RE93;
DT 12-OCT-2022, integrated into UniProtKB/TrEMBL.
DT 12-OCT-2022, sequence version 1.
DT 28-JAN-2026, entry version 11.
DE RecName: Full=CBM20 domain-containing protein {ECO:0000259|PROSITE:PS51166};
GN ORFNames=PVAP13_6KG167400 {ECO:0000313|EMBL:KAG2583380.1};
OS Panicum virgatum (Blackwell switchgrass).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Panicodae; Paniceae; Panicinae; Panicum;
OC Panicum sect. Hiantes.
OX NCBI_TaxID=38727 {ECO:0000313|EMBL:KAG2583380.1, ECO:0000313|Proteomes:UP000823388};
RN [1] {ECO:0000313|EMBL:KAG2583380.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AP13 {ECO:0000313|EMBL:KAG2583380.1};
RA Lovell J.T., Jenkins J., Shu S., Juenger T.E., Schmutz J.;
RT "WGS assembly of Panicum virgatum.";
RL Submitted (MAY-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in intercistronic processing of primary transcripts
CC from chloroplast operons. The endonucleolytic activity of the enzyme
CC depends on the number of phosphates at the 5' end, is inhibited by
CC structured RNA, and preferentially cleaves A/U-rich sequences.
CC {ECO:0000256|ARBA:ARBA00023436}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the RNase E/G family.
CC {ECO:0000256|ARBA:ARBA00005522}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAG2583380.1}.
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DR EMBL; CM029047; KAG2583380.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A8T0RE93; -.
DR Proteomes; UP000823388; Chromosome 6K.
DR GO; GO:0005737; C:cytoplasm; IEA:TreeGrafter.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004540; F:RNA nuclease activity; IEA:InterPro.
DR GO; GO:2001070; F:starch binding; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:TreeGrafter.
DR FunFam; 2.60.40.10:FF:001568; Ribonuclease E/G-like protein, chloroplastic; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.1260.20; Ribonuclease E, catalytic domain; 1.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR002044; CBM20.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR019307; RNA-bd_AU-1/RNase_E/G.
DR InterPro; IPR004659; RNase_E/G.
DR PANTHER; PTHR30001; RIBONUCLEASE; 1.
DR PANTHER; PTHR30001:SF1; RIBONUCLEASE E_G-LIKE PROTEIN, CHLOROPLASTIC; 1.
DR Pfam; PF00686; CBM_20; 1.
DR Pfam; PF10150; RNase_E_G; 1.
DR SMART; SM01065; CBM_2; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 1.
DR PROSITE; PS51166; CBM20; 1.
PE 3: Inferred from homology;
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Reference proteome {ECO:0000313|Proteomes:UP000823388};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 94..204
FT /note="CBM20"
FT /evidence="ECO:0000259|PROSITE:PS51166"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 773..792
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 808 AA; 89373 MW; C9BDD33343645ED2 CRC64;
MAARTLAPPS PLGSMWGPPR APAGSPVTEA SAAAAHAARR GGAAVSPALS GLRGRHSLCS
VQFMDGLGSN IQAEVNLALS PKASMSNGND DSTITRKGFC TISWNLKADI QDGYLIFITG
DPVTLGCWES NMAIQLAPSV ESNNIWTAEI KVPYGVHFKY NYFVREEKGS SSDIIWRPGP
DYSLSIPSVG RKKQFIVVKD LWMKTSVAGL PAPSWGSWLM EAGSLEGEYF EGGNHWSIAE
AHSARDTADQ DLSVDEETHD ASSSFPVENI TDNEEGMTLI PDAKIDIADS AEFEGISGYD
EDKDDENDHM EDEYIEEILQ ADQSEISNDL KTLSSIQHAL RESNDDTNGS RWSQVRKGTK
IMVQVVKEGL GTKGPTLSPF PCLRSRFWIL VSCGNKVGVS KKITGIERTR LKGITKLLRP
PGFTLTARTV AAGHSWEDLQ RDLDGLLSTW KVITEHAQSA ALAAEEGVDG AVPVMLHRAK
GQALSVVQDD FNEKVKRLVV DSPRTYHEVT SYLKEVAPEL CSRVDLYEKR KPIFDEYNIE
KEIDNILCKR VPLNNGGSLV IEQTEALVSI DVNGGHSMFG QGTSQEKAIL EVNLEAAKQI
ARELRLRDIG GIIVVDFIDM TDDLNKRLVY EEMKKAVEKD RSTVGVSELS KLGLMEITRK
RVRPSVTFMI SEPCTCCHGT GRVEALDTSF SKIEREICRR LAASRRKSDP EKPKSWPRFV
LRVDHEMCTY LTSGKKTKLG LLSSSLKVWI LLKIARGFSR GAFELLPYSE KENHEEKETE
SESPQIEGSP KLHVFPIKKW MSRAKRAK
//
