ID A0A8X6HUD9_TRICU Unreviewed; 823 AA.
AC A0A8X6HUD9;
DT 14-DEC-2022, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2022, sequence version 1.
DT 28-JAN-2026, entry version 7.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 16 {ECO:0000256|ARBA:ARBA00019614, ECO:0000256|RuleBase:RU364149};
DE AltName: Full=Mediator complex subunit 16 {ECO:0000256|ARBA:ARBA00032015, ECO:0000256|RuleBase:RU364149};
GN Name=MED16 {ECO:0000256|RuleBase:RU364149,
GN ECO:0000313|EMBL:GFR30346.1};
GN ORFNames=TNCT_480391 {ECO:0000313|EMBL:GFR30346.1};
OS Trichonephila clavata (Joro spider) (Nephila clavata).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Araneoidea; Nephilidae; Trichonephila.
OX NCBI_TaxID=2740835 {ECO:0000313|EMBL:GFR30346.1, ECO:0000313|Proteomes:UP000887116};
RN [1] {ECO:0000313|EMBL:GFR30346.1}
RP NUCLEOTIDE SEQUENCE.
RA Kono N., Nakamura H., Mori M., Yoshida Y., Ohtoshi R., Malay A.D.,
RA Moran D.A.P., Tomita M., Numata K., Arakawa K.;
RT "Multicomponent nature underlies the extraordinary mechanical properties of
RT spider dragline silk.";
RL Submitted (JUL-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. {ECO:0000256|RuleBase:RU364149}.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000256|RuleBase:RU364149}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|RuleBase:RU364149}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 16 family.
CC {ECO:0000256|ARBA:ARBA00006543, ECO:0000256|RuleBase:RU364149}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GFR30346.1}.
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DR EMBL; BMAO01009358; GFR30346.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A8X6HUD9; -.
DR OrthoDB; 10018574at2759; -.
DR Proteomes; UP000887116; Unassembled WGS sequence.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IEA:TreeGrafter.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR048616; MED16_bridge.
DR InterPro; IPR048338; Mediator_Med16.
DR InterPro; IPR048339; Mediator_Med16_C.
DR InterPro; IPR021665; Mediator_Med16_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR13224:SF6; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 16; 1.
DR PANTHER; PTHR13224; THYROID HORMONE RECEPTOR-ASSOCIATED PROTEIN-RELATED; 1.
DR Pfam; PF20718; Med16_bridge; 1.
DR Pfam; PF20719; Med16_C; 1.
DR Pfam; PF11635; Med16_N; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
PE 3: Inferred from homology;
KW Activator {ECO:0000256|ARBA:ARBA00023159, ECO:0000256|RuleBase:RU364149};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU364149};
KW Reference proteome {ECO:0000313|Proteomes:UP000887116};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU364149};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|RuleBase:RU364149}; WD repeat {ECO:0000256|ARBA:ARBA00022574}.
FT DOMAIN 121..395
FT /note="Mediator complex subunit Med16 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF11635"
FT DOMAIN 460..653
FT /note="Mediator of RNA polymerase II transcription subunit
FT 16 central helical bridge"
FT /evidence="ECO:0000259|Pfam:PF20718"
FT DOMAIN 747..817
FT /note="Mediator complex subunit 16 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20719"
SQ SEQUENCE 823 AA; 91919 MW; FB78EF019E04D0A5 CRC64;
MDHIYSVLRR ITPVPSTNTD WITEGKSVIS VSSKNTIAFT STTDLRDGTA KISRIHVYVA
DLNMPWEAHE VLSHNEDITC LEWDISATKL LIADAVGCIQ VWGMKDFLLN EWAQITSTLF
EEYILAAAWF HNGKKIALNM EKKDNPLYLE KYSFIRFGPS VKQFGSKPSE GLIAITTTGM
VFVIILQSDG NIITAAEMLG QFRSKIKVVD LCYAKSGDFL IVTTDGLVQS SVHCYRVGLK
VVQDECIITC EAFSSFFLNC HATCLSGEKQ AYTKVTHLKF ILREAADAVV ITASGPSGST
VELWELREKP VTFHKMFSNV SLERQPKTVV WQHHTSSTSP STVVAMATPR LSIYDASPPP
SYILVAYKDN SIKCFYRESL QLACNISVNT GTHRRDEHTM YSHQQGMKQY LHGAAISDMQ
LSWTGCTLVA IDSLSQLFLY RLCPVTDIGG PMTTSYALTV LEYCLMTGTD WWDVVLSLRP
GWIENLCEKF TDSFNRQPAA AQQGWISRYL SIKGSLYRCL SNGLAKAGDC HALIMLNAIS
AAMKSLLRPR DLSSQDKGPA ENLSAILNSK DTEGVYQMDK VLLHLESKEF TVEPPILQSL
QHLTQWVADC ALYLLATLPY QSPNHNRYPG GGLVSDPKAL NTLRELLVII RIWSLLNESC
LPVFTKMSDN LDVLSLLFKL LTKALLSHGS KPDDSLLDEC NILPNQVLVP IIELGTQAFG
VASPALFMNS LPLQFEYHSQ PEFLRYNSKV HTIEGTIPQN HKIDIVRHVS LGRNPSHVRQ
CTRCYSTSMV KAGARSAATR AWDQRWLRCC PCGGQWQYVT SKS
//
