ID A0A8X7V556_BRACI Unreviewed; 813 AA.
AC A0A8X7V556;
DT 14-DEC-2022, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2022, sequence version 1.
DT 28-JAN-2026, entry version 13.
DE RecName: Full=phosphatidylinositol 3-kinase {ECO:0000256|ARBA:ARBA00012073};
DE EC=2.7.1.137 {ECO:0000256|ARBA:ARBA00012073};
GN ORFNames=Bca52824_031218 {ECO:0000313|EMBL:KAG2302567.1};
OS Brassica carinata (Ethiopian mustard) (Abyssinian cabbage).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=52824 {ECO:0000313|EMBL:KAG2302567.1, ECO:0000313|Proteomes:UP000886595};
RN [1] {ECO:0000313|EMBL:KAG2302567.1, ECO:0000313|Proteomes:UP000886595}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sxm20200214 {ECO:0000313|EMBL:KAG2302567.1};
RC TISSUE=Leaf {ECO:0000313|EMBL:KAG2302567.1};
RA Ma Q., Huang Y., Song X., Pei D.;
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a
CC 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP +
CC H(+); Xref=Rhea:RHEA:12709, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:57880, ChEBI:CHEBI:58088, ChEBI:CHEBI:456216;
CC EC=2.7.1.137; Evidence={ECO:0000256|ARBA:ARBA00001498};
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family.
CC {ECO:0000256|PIRNR:PIRNR000587, ECO:0000256|PROSITE-ProRule:PRU00880}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAG2302567.1}.
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DR EMBL; JAAMPC010000007; KAG2302567.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A8X7V556; -.
DR OrthoDB; 67688at2759; -.
DR Proteomes; UP000886595; Unassembled WGS sequence.
DR GO; GO:0005768; C:endosome; IEA:TreeGrafter.
DR GO; GO:0005777; C:peroxisome; IEA:TreeGrafter.
DR GO; GO:0000407; C:phagophore assembly site; IEA:TreeGrafter.
DR GO; GO:0034271; C:phosphatidylinositol 3-kinase complex, class III, type I; IEA:TreeGrafter.
DR GO; GO:0034272; C:phosphatidylinositol 3-kinase complex, class III, type II; IEA:TreeGrafter.
DR GO; GO:0016303; F:1-phosphatidylinositol-3-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000045; P:autophagosome assembly; IEA:TreeGrafter.
DR GO; GO:0006897; P:endocytosis; IEA:TreeGrafter.
DR GO; GO:0048015; P:phosphatidylinositol-mediated signaling; IEA:TreeGrafter.
DR CDD; cd08397; C2_PI3K_class_III; 1.
DR CDD; cd00870; PI3Ka_III; 1.
DR CDD; cd00896; PI3Kc_III; 1.
DR FunFam; 3.30.1010.10:FF:000002; Phosphatidylinositol 3-kinase catalytic subunit type 3; 1.
DR FunFam; 2.60.40.150:FF:000171; Phosphatidylinositol 3-kinase VPS34; 1.
DR FunFam; 1.10.1070.11:FF:000014; Phosphatidylinositol 3-kinase, root isoform; 1.
DR FunFam; 1.25.40.70:FF:000012; phosphatidylinositol 3-kinase, root isoform; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR Gene3D; 3.30.1010.10; Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4; 1.
DR Gene3D; 1.25.40.70; Phosphatidylinositol 3-kinase, accessory domain (PIK); 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR057756; PI3-kinase_type3/VPS34_cat.
DR InterPro; IPR015433; PI3/4_kinase.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR002420; PI3K-type_C2_dom.
DR InterPro; IPR001263; PI3K_accessory_dom.
DR InterPro; IPR042236; PI3K_accessory_sf.
DR InterPro; IPR008290; PI3K_Vps34.
DR PANTHER; PTHR10048:SF7; PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3; 1.
DR PANTHER; PTHR10048; PHOSPHATIDYLINOSITOL KINASE; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR Pfam; PF00792; PI3K_C2; 1.
DR Pfam; PF00613; PI3Ka; 1.
DR PIRSF; PIRSF000587; PI3K_Vps34; 1.
DR SMART; SM00142; PI3K_C2; 1.
DR SMART; SM00145; PI3Ka; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51547; C2_PI3K; 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
DR PROSITE; PS51545; PIK_HELICAL; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR000587};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|PIRNR:PIRNR000587};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR000587};
KW Reference proteome {ECO:0000313|Proteomes:UP000886595};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR000587}.
FT DOMAIN 14..177
FT /note="C2 PI3K-type"
FT /evidence="ECO:0000259|PROSITE:PS51547"
FT DOMAIN 274..449
FT /note="PIK helical"
FT /evidence="ECO:0000259|PROSITE:PS51545"
FT DOMAIN 532..798
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
SQ SEQUENCE 813 AA; 93303 MW; F4A7CDA4CF20E784 CRC64;
MGASEFRFFL SCDLNSPVTF RIEKLDGILP LHKSSDSGVV SGVEDKRPEL YIECALYIDG
APFGLPMRTR LNTTGPPYCW NELITLSSKY RDLTAHSQLA ITVWDVSCGK AEGLIGGATI
LLFNSKMQMK SGKQKLRLWQ GKEADGSFPT STPGKVPRHE RGELERLEKL MNKFERGQIQ
SIDWLDRLML RSLDKIKEQE SSKHGNSYLY LVVDFCSFEH RVVFQESGAN LLITSPIGST
NEFVTVWDTE LGKFNPSEHK QLKLARSLDR GIIDRDLKPS NNERKSIQRV LKYPPTRTLS
GDERQLLWKF RFSLMSEKRA LTKFLRCVEW SDVQEAKQAI QLMYKWETID VCDALELLSP
LFESEEVRAY AVSVLERADD EELQCYLLQL VQALRFERSD RSRLSQFLVQ RALQNIQLAS
FFRWYVAVEL TDHVYNKRYF STYDLLEQSM KKLPPDVNGE DGNKLWQSLV GQTGLTAQLV
SITREVRNVR GNTQKKIEKL RQLLSGLLSE LTYFEEPIRS PLTPSVLIKG IVPGESTLFK
SQLNPLLLAF RTEDEGSCKV IFKKGDDLRQ DQLVVQMVWL MDRLLKLENL DLCLTPYKVL
ATGHDEGMLE FIPSRSLAQI LSEHRSITSY LQKFHPDEHA PFGITATCLE TFIKSCAGYS
VITYILGIGD RHLDNLLLTD DGRLFHVDFA FILGRDPKPF PPPMKLCKEM VEAMGGAESQ
YYTRFKSYCC EAYNILRKSS NLILNLFYLM AGSTIPDIAS DPEKGILKLQ EKFRLDMDDE
ACIHFFQDLI NESVSALFPQ MVETIHRWAQ YWR
//
