ID A0A8X8B3S3_BRACI Unreviewed; 575 AA.
AC A0A8X8B3S3;
DT 14-DEC-2022, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2022, sequence version 1.
DT 28-JAN-2026, entry version 9.
DE RecName: Full=RNA polymerase sigma factor {ECO:0000256|PIRNR:PIRNR000767};
GN ORFNames=Bca52824_013325 {ECO:0000313|EMBL:KAG2320112.1};
OS Brassica carinata (Ethiopian mustard) (Abyssinian cabbage).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=52824 {ECO:0000313|EMBL:KAG2320112.1, ECO:0000313|Proteomes:UP000886595};
RN [1] {ECO:0000313|EMBL:KAG2320112.1, ECO:0000313|Proteomes:UP000886595}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sxm20200214 {ECO:0000313|EMBL:KAG2320112.1};
RC TISSUE=Leaf {ECO:0000313|EMBL:KAG2320112.1};
RA Ma Q., Huang Y., Song X., Pei D.;
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of plastid-encoded RNA polymerase (PEP) to specific
CC initiation sites and are then released.
CC {ECO:0000256|PIRNR:PIRNR000767}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000256|PIRNR:PIRNR000767}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family.
CC {ECO:0000256|ARBA:ARBA00007788, ECO:0000256|PIRNR:PIRNR000767}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAG2320112.1}.
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DR EMBL; JAAMPC010000003; KAG2320112.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A8X8B3S3; -.
DR OrthoDB; 206108at2759; -.
DR Proteomes; UP000886595; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0071482; P:cellular response to light stimulus; IEA:UniProtKB-ARBA.
DR GO; GO:0006352; P:DNA-templated transcription initiation; IEA:UniProtKB-UniRule.
DR CDD; cd06171; Sigma70_r4; 1.
DR FunFam; 1.10.601.10:FF:000007; RNA polymerase sigma factor sigB; 1.
DR Gene3D; 1.10.601.10; RNA Polymerase Primary Sigma Factor; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 2.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR016262; RNA_pol_sigma_SigB/C/D/F.
DR InterPro; IPR050239; Sigma-70_RNA_pol_init_factors.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR NCBIfam; TIGR02937; sigma70-ECF; 1.
DR PANTHER; PTHR30603; RNA POLYMERASE SIGMA FACTOR RPO; 1.
DR PANTHER; PTHR30603:SF57; RNA POLYMERASE SIGMA FACTOR SIGB; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PIRSF; PIRSF000767; RNA_pol_sigma_SigB/C/D; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88946; Sigma2 domain of RNA polymerase sigma factors; 1.
DR SUPFAM; SSF88659; Sigma3 and sigma4 domains of RNA polymerase sigma factors; 2.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000256|PIRNR:PIRNR000767};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PIRNR:PIRNR000767};
KW Plastid {ECO:0000256|PIRNR:PIRNR000767};
KW Reference proteome {ECO:0000313|Proteomes:UP000886595};
KW Sigma factor {ECO:0000256|ARBA:ARBA00023082,
KW ECO:0000256|PIRNR:PIRNR000767};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|PIRNR:PIRNR000767};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|PIRNR:PIRNR000767}.
FT DOMAIN 533..559
FT /note="RNA polymerase sigma-70"
FT /evidence="ECO:0000259|PROSITE:PS00716"
FT REGION 176..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 187..202
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 575 AA; 64304 MW; 73D0731D6345F957 CRC64;
MSSCLLPQFK CPPDPFSIQF RTSHSVPKHN KGSSVFFQPQ CAVSTSPPLL TSVLDVAKLR
LPSFDTDSNP RISDRQQWTY TGTIGPSTEA KYLEAETLLT SDEAVVAAAA AEAVALARAA
VKVAKDATLF GNSHNAKLLT SPTGAGEKRS KWDQFTEKER AGILGHLAVS DTGVVSDRVT
SPAVPECNKE SSDDIESEKQ QEVEPLEEEL SVSSAVRSTR QTERKARRAK GLEKTASGMQ
SVKISSSSRK KRVASQEIDH NDPLRYLRMT TSSSKLLTAR EETELSEGIQ DLLKLERLQA
ELTERCGHQP TFGQWASAAG VDQKTLRKRI NHGTQCKDRM IKSNIRLVIS IAKNYQGAGM
NLQDLVQEGC RGLVRGAEKF DATKGFKFST YAHWWIKQAV RKSLSDQSRM IRLPFHMVEA
TYRVKEARKQ LYSETGQQPK NEEVAEATGL SMKRLMAVLL SPKPPRSLDQ KIGINQNLKP
SEVISDPEAE TSEDILIKQF MREDLDKVLD SLGTREKQVI RWRFGLEDGR MKTLQEIGET
MGVSRERVRQ IESSAFRKLK NKKRNNHLQQ YLVAQ
//
