ID A0A915PMN9_9BILA Unreviewed; 746 AA.
AC A0A915PMN9;
DT 22-FEB-2023, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2023, sequence version 1.
DT 28-JAN-2026, entry version 14.
DE RecName: Full=Vesicle-fusing ATPase {ECO:0000256|ARBA:ARBA00012674, ECO:0000256|RuleBase:RU367045};
DE EC=3.6.4.6 {ECO:0000256|ARBA:ARBA00012674, ECO:0000256|RuleBase:RU367045};
OS Setaria digitata.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Spirurina; Spiruromorpha; Filarioidea; Setariidae; Setaria.
OX NCBI_TaxID=48799 {ECO:0000313|Proteomes:UP000887581, ECO:0000313|WBParaSite:sdigi.contig145.g5203.t1};
RN [1] {ECO:0000313|WBParaSite:sdigi.contig145.g5203.t1}
RP IDENTIFICATION.
RG WormBaseParasite;
RL Submitted (NOV-2022) to UniProtKB.
CC -!- FUNCTION: Required for vesicle-mediated transport. Catalyzes the fusion
CC of transport vesicles within the Golgi cisternae. Is also required for
CC transport from the endoplasmic reticulum to the Golgi stack. Seems to
CC function as a fusion protein required for the delivery of cargo
CC proteins to all compartments of the Golgi stack independent of vesicle
CC origin. {ECO:0000256|ARBA:ARBA00056429, ECO:0000256|RuleBase:RU367045}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.6;
CC Evidence={ECO:0000256|ARBA:ARBA00048883,
CC ECO:0000256|RuleBase:RU367045};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU367045};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|RuleBase:RU367045};
CC -!- SUBUNIT: Homohexamer. {ECO:0000256|ARBA:ARBA00011643}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|RuleBase:RU367045}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family.
CC {ECO:0000256|ARBA:ARBA00006914, ECO:0000256|RuleBase:RU367045}.
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DR AlphaFoldDB; A0A915PMN9; -.
DR WBParaSite; sdigi.contig145.g5203.t1; sdigi.contig145.g5203.t1; sdigi.contig145.g5203.
DR Proteomes; UP000887581; Unplaced.
DR GO; GO:0005795; C:Golgi stack; IEA:TreeGrafter.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; IEA:TreeGrafter.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IEA:TreeGrafter.
DR GO; GO:0035494; P:SNARE complex disassembly; IEA:InterPro.
DR CDD; cd19504; RecA-like_NSF-SEC18_r1-like; 1.
DR FunFam; 1.10.8.60:FF:000026; vesicle-fusing ATPase isoform X1; 1.
DR FunFam; 3.40.50.300:FF:000166; vesicle-fusing ATPase isoform X1; 1.
DR FunFam; 2.40.40.20:FF:000012; Vesicle-fusing ATPase protein; 1.
DR FunFam; 3.40.50.300:FF:000187; Vesicular-fusion ATPase SEC18; 1.
DR Gene3D; 1.10.8.60; -; 2.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.10.330.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR029067; CDC48_domain_2-like_sf.
DR InterPro; IPR003338; CDC4_N-term_subdom.
DR InterPro; IPR054419; NSF_ATPase_lid.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR039812; Vesicle-fus_ATPase.
DR PANTHER; PTHR23078:SF3; VESICLE-FUSING ATPASE; 1.
DR PANTHER; PTHR23078; VESICULAR-FUSION PROTEIN NSF; 1.
DR Pfam; PF00004; AAA; 2.
DR Pfam; PF17862; AAA_lid_3; 1.
DR Pfam; PF02359; CDC48_N; 1.
DR Pfam; PF21964; NSF_ATPase_lid; 1.
DR SMART; SM00382; AAA; 2.
DR SMART; SM01073; CDC48_N; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF54585; Cdc48 domain 2-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00674; AAA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU367045};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU367045};
KW ER-Golgi transport {ECO:0000256|ARBA:ARBA00022892,
KW ECO:0000256|RuleBase:RU367045}; Hydrolase {ECO:0000256|RuleBase:RU367045};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU367045};
KW Metal-binding {ECO:0000256|RuleBase:RU367045};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU367045};
KW Protein transport {ECO:0000256|ARBA:ARBA00022927,
KW ECO:0000256|RuleBase:RU367045};
KW Reference proteome {ECO:0000313|Proteomes:UP000887581};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU367045}.
FT DOMAIN 1..82
FT /note="CDC48 N-terminal subdomain"
FT /evidence="ECO:0000259|SMART:SM01073"
FT DOMAIN 255..402
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 538..674
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT COILED 680..707
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 746 AA; 82552 MW; 6301B7DE5DCD34C6 CRC64;
MRVRKCPTDD LALTNCAILN ISALNGKEIK HILVKTGPAH HFVFSIRNHP SLKADEIAFA
LSQRKWAKLS LDQEVEVQQF IFNNNQFIGS ITVAADFQSK KNQTPEPLNT DHMAREFSIQ
FAGHAFTRGE LLVFKFDDDK GKSHTLALTV TSILGIDLSL ATNPQAVSHL KPAEIDSGQL
LPNSVIIFDK AEDSLLNLIG KSKGKSAYRS IINPDWDFQK MGIGGLDKEF SGIFRRAFAS
RVFPPEFIEQ LGMKHVRGIL LYGPPGTGKT LMARQIGKML NAREPKIING PQILDKYVGE
SESNIRKLFA DAEEEWKRCG ASSGLHIIIF DEIDAICKQR GSVAGSTAVH DTVVNQLLAK
MDGVDQLNNI LVIGMTNRKD MIDEALLRPG RMEVQMEISL PDEAGRLQIL KIHTARMREY
DKLDPNVDLI ELAKKTKNFS GAELEGLVRA AQSSAMNRLV KAGGKVQLDA DAVEKLMINV
DDFNYALEND VKPAFGHSNE ELEKYLIGGF ISWSAQVAQI LEQGALLVKQ VRSPDTKGFA
SVLLAGSPNS GKTCFAAMIA KASDYPFIKV ISAEDMVGYT ETAKCATLRK VFDDAYRSPL
SCVIVDGVER LLDFSPIGPR YSNLVLQALF LLVKKLPPQN RRLLVIATSS NRSFLRELGL
LSAFCTIIDV PALTTVQHIM AVIEDTNALS RQECEEIRNE LSRANKEYFI GIKKLLNVVD
MARECEPVDR VSVVVQSLMS ETFSNS
//
