ID A0A917TSM6_9ACTN Unreviewed; 322 AA.
AC A0A917TSM6;
DT 22-FEB-2023, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2023, sequence version 1.
DT 28-JAN-2026, entry version 12.
DE SubName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (Flavodoxin) 1 {ECO:0000313|EMBL:GGM36313.1};
GN Name=ispG1 {ECO:0000313|EMBL:GGM36313.1};
GN ORFNames=GCM10011608_21200 {ECO:0000313|EMBL:GGM36313.1};
OS Micromonospora sonchi.
OC Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Micromonospora.
OX NCBI_TaxID=1763543 {ECO:0000313|EMBL:GGM36313.1, ECO:0000313|Proteomes:UP000608890};
RN [1] {ECO:0000313|EMBL:GGM36313.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CGMCC 4.7312 {ECO:0000313|EMBL:GGM36313.1};
RX PubMed=25193709;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Walter F., Albersmeier A., Kalinowski J., Ruckert C.;
RT "Complete genome sequence of Corynebacterium casei LMG S-19264T (=DSM
RT 44701T), isolated from a smear-ripened cheese.";
RL Int. J. Syst. Evol. Microbiol. 189:76-77(2014).
RN [2] {ECO:0000313|EMBL:GGM36313.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CGMCC 4.7312 {ECO:0000313|EMBL:GGM36313.1};
RA Sun Q., Zhou Y.;
RL Submitted (SEP-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GGM36313.1}.
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DR EMBL; BMNB01000008; GGM36313.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A917TSM6; -.
DR Proteomes; UP000608890; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046429; F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity (ferredoxin); IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway; IEA:TreeGrafter.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:InterPro.
DR Gene3D; 3.20.20.20; Dihydropteroate synthase-like; 1.
DR Gene3D; 3.30.413.10; Sulfite Reductase Hemoprotein, domain 1; 1.
DR InterPro; IPR011005; Dihydropteroate_synth-like_sf.
DR InterPro; IPR016425; IspG_bac.
DR InterPro; IPR004588; IspG_bac-typ.
DR InterPro; IPR058579; IspG_C.
DR InterPro; IPR058578; IspG_TIM.
DR InterPro; IPR045854; NO2/SO3_Rdtase_4Fe4S_sf.
DR NCBIfam; TIGR00612; ispG_gcpE; 1.
DR NCBIfam; NF001540; PRK00366.1; 1.
DR PANTHER; PTHR30454; 4-HYDROXY-3-METHYLBUT-2-EN-1-YL DIPHOSPHATE SYNTHASE; 1.
DR PANTHER; PTHR30454:SF0; 4-HYDROXY-3-METHYLBUT-2-EN-1-YL DIPHOSPHATE SYNTHASE (FERREDOXIN), CHLOROPLASTIC; 1.
DR Pfam; PF04551; GcpE; 1.
DR Pfam; PF26540; GcpE_C; 1.
DR PIRSF; PIRSF004640; IspG; 1.
DR SUPFAM; SSF56014; Nitrite and sulphite reductase 4Fe-4S domain-like; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Isoprene biosynthesis {ECO:0000256|ARBA:ARBA00023229};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000608890}.
FT DOMAIN 1..194
FT /note="IspG TIM-barrel"
FT /evidence="ECO:0000259|Pfam:PF04551"
FT DOMAIN 209..294
FT /note="IspG C-terminal"
FT /evidence="ECO:0000259|Pfam:PF26540"
SQ SEQUENCE 322 AA; 34033 MW; 600DE3BBC9EBFEF1 CRC64;
MRVAVPSQDD VEALPAIAKK SQIPVIADIH FQPKYVFAAI DAGCAAVRVN PGNIRQFDDK
VKEIAAAASA TGTPIRIGVN AGSLDKRLLA KYGKATAEAM VESALWECSL FEEHGFRDIK
ISVKHNDPVV MIRAYRQLAE QCDYPLHLGV TEAGPAFQGT IKSAVAFGAL LAEGIGDTIR
VSLSAPPVEE IKVGNQILES LGLRERGLEI VSCPSCGRAQ VDVYKLAAEV TAGLEGLPVP
LRVAVMGCVV NGPGEAREAD LGVASGNGKG QIFVKGQVIK TVPEGQIVET LIEEALRLAD
EMGADIPEEL RTLLPGATVT VH
//
