ID A0A969WEP2_9GAMM Unreviewed; 698 AA.
AC A0A969WEP2;
DT 22-FEB-2023, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2023, sequence version 1.
DT 18-JUN-2025, entry version 14.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00044770};
DE EC=2.4.99.28 {ECO:0000256|ARBA:ARBA00044770};
GN ORFNames=G7Y82_13700 {ECO:0000313|EMBL:NKF23370.1};
OS Solimonas marina.
OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Nevskiales;
OC Nevskiaceae; Solimonas.
OX NCBI_TaxID=2714601 {ECO:0000313|EMBL:NKF23370.1, ECO:0000313|Proteomes:UP000653472};
RN [1] {ECO:0000313|EMBL:NKF23370.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C16B3 {ECO:0000313|EMBL:NKF23370.1};
RA Liu X., Lai Q., Sun F., Gai Y., Li G., Shao Z.;
RT "Solimonas marina sp. nov., isolated from deep seawater of the Pacific
RT Ocean.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans,octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.99.28;
CC Evidence={ECO:0000256|ARBA:ARBA00049902};
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004752}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the transpeptidase
CC family. {ECO:0000256|ARBA:ARBA00007090}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the
CC glycosyltransferase 51 family. {ECO:0000256|ARBA:ARBA00007739}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:NKF23370.1}.
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DR EMBL; JAAVXB010000007; NKF23370.1; -; Genomic_DNA.
DR RefSeq; WP_168148686.1; NZ_JAAVXB010000007.1.
DR Proteomes; UP000653472; Unassembled WGS sequence.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:TreeGrafter.
DR GO; GO:0004180; F:carboxypeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:TreeGrafter.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:TreeGrafter.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR050396; Glycosyltr_51/Transpeptidase.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR009647; PBP_C.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF15; PENICILLIN-BINDING PROTEIN 1C; 1.
DR Pfam; PF06832; BiPBP_C; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 3: Inferred from homology;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022670};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000653472};
KW Transferase {ECO:0000256|ARBA:ARBA00022676}.
FT DOMAIN 69..232
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 312..550
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
FT DOMAIN 605..679
FT /note="Penicillin-binding C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06832"
SQ SEQUENCE 698 AA; 76413 MW; F6B26A50A6388C3A CRC64;
MSVRAVAAAL RRRPLRSAVG ALVALLVLAT VLSQRALPDT LAPRVAAGDD IVLLDRDGRP
LQLAYDGGWN RQHRVPLERI PATLRTAFVA SEDRRFWRHH GVDWPGRVAA LWTDLRAGRA
VRGASTISEQ VVRILHPRPR TVWSRWVEGF EAYRLEARYS KADILEFYLN QVPYGANRRG
VAQAARYYYG RSLDTLSTEE MLALAVLVRA PTRLANEPLA LQSRVRRLAG AMQARGELPA
DVAMSTVPDF VRAQPALHAA YFRAFAERQA QQLRPSAAQL RTTLDPALQA AAEAFLQERL
RDLARDGVGQ AAALVVDLDG NRVRAYASAD RLHPNVVGID PLQTPRQPGS TLKPLLYAQA
LERGWRADTT IDDAELRERI NGGLHEYRNY SRTHYGTVTV AEALGNSLNI PAVKALQFVG
QAPFLARLQA LGVQSLTEPP DYYGDGLALG NGALTLYELV QAYTALAHDG QWQPLRVIED
AGPQAPPRQV IARDAARTIS AVLADASSRL LEFGDGGVLR FPVRTAVKTG TSSDYRDAWT
VAYNGRWLVG VWVGNLSGVA TDGITGARGP ALLTRSLLAG LSSDTPLRAA TLHLDPQADA
QAAVDALPST PRIAQPFDGL QLARDPRIPD DLEVLRFELA WRGTPTTVRW EVDGRVVAQT
PGTQYDWPLQ AGVHRLRAIV TTPDADDVST PTVHFRVR
//
