ID   A0A974XEB8_9FIRM        Unreviewed;       639 AA.
AC   A0A974XEB8;
DT   22-FEB-2023, integrated into UniProtKB/TrEMBL.
DT   22-FEB-2023, sequence version 1.
DT   28-JAN-2026, entry version 15.
DE   RecName: Full=Selenocysteine-specific elongation factor {ECO:0000256|ARBA:ARBA00015953};
DE   AltName: Full=SelB translation factor {ECO:0000256|ARBA:ARBA00031615};
GN   Name=selB {ECO:0000313|EMBL:QSX08263.1};
GN   ORFNames=J0B03_10790 {ECO:0000313|EMBL:QSX08263.1};
OS   Alkalibacter rhizosphaerae.
OC   Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC   Alkalibacter.
OX   NCBI_TaxID=2815577 {ECO:0000313|EMBL:QSX08263.1, ECO:0000313|Proteomes:UP000663499};
RN   [1] {ECO:0000313|EMBL:QSX08263.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ES005 {ECO:0000313|EMBL:QSX08263.1};
RA   Namirimu T., Yang J.-A., Yang S.-H., Kim Y.-J., Kwon K.K.;
RT   "Alkalibacter marinus sp. nov., isolated from tidal flat sediment.";
RL   Submitted (MAR-2021) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Translation factor necessary for the incorporation of
CC       selenocysteine into proteins. It probably replaces EF-Tu for the
CC       insertion of selenocysteine directed by the UGA codon. SelB binds GTP
CC       and GDP. {ECO:0000256|ARBA:ARBA00025526}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR   EMBL; CP071444; QSX08263.1; -; Genomic_DNA.
DR   RefSeq; WP_207299605.1; NZ_CP071444.1.
DR   AlphaFoldDB; A0A974XEB8; -.
DR   KEGG; alka:J0B03_10790; -.
DR   Proteomes; UP000663499; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR   GO; GO:0001514; P:selenocysteine incorporation; IEA:InterPro.
DR   CDD; cd04171; SelB; 1.
DR   CDD; cd03696; SelB_II; 1.
DR   CDD; cd15491; selB_III; 1.
DR   Gene3D; 1.10.10.2770; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   Gene3D; 2.40.30.10; Translation factors; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR057335; Beta-barrel_SelB.
DR   InterPro; IPR000591; DEP_dom.
DR   InterPro; IPR050055; EF-Tu_GTPase.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR015190; Elong_fac_SelB-wing-hlx_typ-2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR015191; SelB_WHD4.
DR   InterPro; IPR005225; Small_GTP-bd.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR   InterPro; IPR004535; Transl_elong_SelB.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   NCBIfam; TIGR00475; selB; 1.
DR   NCBIfam; TIGR00231; small_GTP; 1.
DR   PANTHER; PTHR43721:SF22; ELONGATION FACTOR TU, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43721; ELONGATION FACTOR TU-RELATED; 1.
DR   Pfam; PF25461; Beta-barrel_SelB; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF09106; WHD_2nd_SelB; 1.
DR   Pfam; PF09107; WHD_3rd_SelB; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   SUPFAM; SSF50447; Translation proteins; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 2.
DR   PROSITE; PS50186; DEP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   4: Predicted;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Elongation factor {ECO:0000313|EMBL:QSX08263.1};
KW   GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW   Reference proteome {ECO:0000313|Proteomes:UP000663499}.
FT   DOMAIN          1..177
FT                   /note="Tr-type G"
FT                   /evidence="ECO:0000259|PROSITE:PS51722"
FT   DOMAIN          378..454
FT                   /note="DEP"
FT                   /evidence="ECO:0000259|PROSITE:PS50186"
SQ   SEQUENCE   639 AA;  71578 MW;  3BEE056C9DAF1DE1 CRC64;
     MKHITIGTAG HIDHGKTALV KRLTGTDTDR LAEEQDRGMT IELGFAFLQL ASGNQVSIVD
     VPGHEKFVKT MVAGVTGIDI VLLIIAADEG IMPQTIEHLD ILSVLGLSKG VVALTKSDLV
     DQKTLDQRIR EIRKYLKDTP LESLPVIPVS SKTGAGIDDL LIKIDELAIQ TREKSPTSLF
     RLPVDRVFTM TGHGTIVTGT ISGGTVTKGD PVQILPEGLT TKVRNIQVRN QSVDQAYGGD
     RCALNLASIE KKDLQRGSVV LESGNFRTTS LLDARVQMVP GSNGLVHNQR VHFHTGTTAV
     LARVRLLGAD KIDAGEKGYV QLRLEEPVAA FRADRFILRS FSPVHTIGGG RLLLHHTRNR
     KRFDSTSMKE LAFLDLGTDK EVIQWLIQSS SSLMDVESIY LETLIDRDRI KEMLEELVVE
     GVIYAMGSTG KYYSLAWCDE MLKELEGVFH RFYNKNPYRY QISKEALRKK EFPFLSNKDF
     DGMLEIMSAM SSLRLEEKFV HLHKEKRLDE ISDLVEVART RQLFETPDLP TLSPAQVAEE
     TSLPIAVAEE ILHFFISIGY LVELEKGVFC SRRSLEMIYQ TVVDAIQKEK SITVARLRDL
     LGTGRKTAIY FLEYFDAVRV TKRDGNDRIP GPRFRDFLH
//