ID A0A9D5APN2_PEA Unreviewed; 703 AA.
AC A0A9D5APN2;
DT 03-MAY-2023, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2023, sequence version 1.
DT 28-JAN-2026, entry version 13.
DE RecName: Full=leucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013164};
DE EC=6.1.1.4 {ECO:0000256|ARBA:ARBA00013164};
GN ORFNames=KIW84_040150 {ECO:0000313|EMBL:KAI5414549.1};
OS Pisum sativum (Garden pea) (Lathyrus oleraceus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Fabeae; Lathyrus.
OX NCBI_TaxID=3888 {ECO:0000313|EMBL:KAI5414549.1, ECO:0000313|Proteomes:UP001058974};
RN [1] {ECO:0000313|EMBL:KAI5414549.1, ECO:0000313|Proteomes:UP001058974}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Zhongwan 6 {ECO:0000313|EMBL:KAI5414549.1,
RC ECO:0000313|Proteomes:UP001058974};
RX PubMed=36138232; DOI=10.1038/s41588-022-01172-2;
RA Yang T., Liu R., Luo Y., Hu S., Wang D., Wang C., Pandey M.K., Ge S.,
RA Xu Q., Li N., Li G., Huang Y., Saxena R.K., Ji Y., Li M., Yan X., He Y.,
RA Liu Y., Wang X., Xiang C., Varshney R.K., Ding H., Gao S., Zong X.;
RT "Improved pea reference genome and pan-genome highlight genomic features
RT and evolutionary characteristics.";
RL Nat. Genet. 54:1553-1563(2022).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP +
CC diphosphate; Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00047469};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAI5414549.1}.
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DR EMBL; JAMSHJ010000004; KAI5414549.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A9D5APN2; -.
DR Gramene; Psat04G0015000-T4; KAI5414549.1; KIW84_040150.
DR Proteomes; UP001058974; Chromosome 4.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-ARBA.
DR GO; GO:0002161; F:aminoacyl-tRNA deacylase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07958; Anticodon_Ia_Leu_BEm; 1.
DR FunFam; 3.40.50.620:FF:000056; Leucine--tRNA ligase; 1.
DR FunFam; 1.10.730.10:FF:000011; Leucine--tRNA ligase chloroplastic/mitochondrial; 1.
DR FunFam; 3.90.740.10:FF:000049; Os01g0120300 protein; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.730.10; Isoleucyl-tRNA Synthetase, Domain 1; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00396; leuS_bact; 1.
DR PANTHER; PTHR43740:SF2; LEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43740; LEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP001058974}.
FT DOMAIN 33..225
FT /note="Leucyl-tRNA synthetase editing"
FT /evidence="ECO:0000259|Pfam:PF13603"
FT DOMAIN 241..401
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 458..488
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 544..651
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 703 AA; 79213 MW; 15CF546C7D8C4469 CRC64;
MRQWMLKITA YADRLLEDLD DLDWPESVKE MQRNWIGRSE GAELEFCILD GDGKEKDMQI
IVYTTRPDTI FGATYLVVAP EHPLLSSLAS TAQSNHMEDY IDLASRKSDL ERTELQKEKT
GVFTGCYAKN PANGEAIPIW VADYVLGSYG TGAIMAVPAH DSRDYEFALK YDIPVRWVVM
PDDKSMNESG KAFPGEGIIV NSSDTLTGLD INGLCSKEAA LKVIDWAEHG GNGKRKVNYK
LRDWLFARQR YWGEPIPVIF LDDSGETVPL DETELPLILP ELDDFSPSGT GEPPLSKAVT
WVKTTDRLSG RPATRETNTM PQWAGSCWYY LRFMDPNNSK ELVDKAKERY WGPVDVYVGG
AEHAVLHLLY ARFWHKVLFD IGVVSTKEPF QCVINQGIIL GEVQYMACRD KDGNLISADS
TNKLNENNLE RISEEKVTKS GDSFVLKENP EIRLVARAHK MSKSRGNVVN PDDVISEYGA
DSLRLYEMFM GPLRDSKTWS TGGIEGVYRF LGRTWRLIVG LPLSDGTFKD RTVSVDEEPT
IEQLRCLHKC IAKVTEEIEG TRFNTGISAM MEFLNAAYKW DKHPRSVVEA FVLLLSPYAP
HMAEELWSRL GHTKSLAYEP FPKANPAYLK DSIIVLPVQI NGKTRGTIQV EETCTEEDAF
ALASKDEKLS KYLDGQSVRK RIYVPGKILN VVLDRKNVKV GVQ
//
