ID A0A9D5BH81_PEA Unreviewed; 740 AA.
AC A0A9D5BH81;
DT 03-MAY-2023, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2023, sequence version 1.
DT 18-JUN-2025, entry version 11.
DE RecName: Full=valine--tRNA ligase {ECO:0000256|ARBA:ARBA00013169};
DE EC=6.1.1.9 {ECO:0000256|ARBA:ARBA00013169};
DE AltName: Full=Valyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00029936};
GN ORFNames=KIW84_012304 {ECO:0000313|EMBL:KAI5443605.1};
OS Pisum sativum (Garden pea) (Lathyrus oleraceus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX NCBI_TaxID=3888 {ECO:0000313|EMBL:KAI5443605.1, ECO:0000313|Proteomes:UP001058974};
RN [1] {ECO:0000313|EMBL:KAI5443605.1, ECO:0000313|Proteomes:UP001058974}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Zhongwan 6 {ECO:0000313|EMBL:KAI5443605.1,
RC ECO:0000313|Proteomes:UP001058974};
RX PubMed=36138232; DOI=10.1038/s41588-022-01172-2;
RA Yang T., Liu R., Luo Y., Hu S., Wang D., Wang C., Pandey M.K., Ge S.,
RA Xu Q., Li N., Li G., Huang Y., Saxena R.K., Ji Y., Li M., Yan X., He Y.,
RA Liu Y., Wang X., Xiang C., Varshney R.K., Ding H., Gao S., Zong X.;
RT "Improved pea reference genome and pan-genome highlight genomic features
RT and evolutionary characteristics.";
RL Nat. Genet. 54:1553-1563(2022).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP +
CC diphosphate; Xref=Rhea:RHEA:10704, Rhea:RHEA-COMP:9672, Rhea:RHEA-
CC COMP:9708, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57762,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78537, ChEBI:CHEBI:456215; EC=6.1.1.9;
CC Evidence={ECO:0000256|ARBA:ARBA00047552};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAI5443605.1}.
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DR EMBL; JAMSHJ010000001; KAI5443605.1; -; Genomic_DNA.
DR Proteomes; UP001058974; Chromosome 1.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0002161; F:aminoacyl-tRNA deacylase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004832; F:valine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0006438; P:valyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07962; Anticodon_Ia_Val; 1.
DR FunFam; 1.10.287.380:FF:000001; Valine--tRNA ligase; 1.
DR FunFam; 1.10.730.10:FF:000014; Valine--tRNA ligase; 1.
DR FunFam; 3.40.50.620:FF:000126; Valine--tRNA ligase chloroplastic/mitochondrial 2; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 1.10.730.10; Isoleucyl-tRNA Synthetase, Domain 1; 1.
DR Gene3D; 1.10.287.380; Valyl-tRNA synthetase, C-terminal domain; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033705; Anticodon_Ia_Val.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR010978; tRNA-bd_arm.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR037118; Val-tRNA_synth_C_sf.
DR InterPro; IPR019499; Val-tRNA_synth_tRNA-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR InterPro; IPR002303; Valyl-tRNA_ligase.
DR NCBIfam; NF004349; PRK05729.1; 1.
DR NCBIfam; TIGR00422; valS; 1.
DR PANTHER; PTHR11946:SF93; VALINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR PANTHER; PTHR11946; VALYL-TRNA SYNTHETASES; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF10458; Val_tRNA-synt_C; 1.
DR PRINTS; PR00986; TRNASYNTHVAL.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF46589; tRNA-binding arm; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 4: Predicted;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP001058974}.
FT DOMAIN 1..402
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 457..604
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT DOMAIN 668..731
FT /note="Valyl-tRNA synthetase tRNA-binding arm"
FT /evidence="ECO:0000259|Pfam:PF10458"
FT COILED 666..735
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 740 AA; 84384 MW; 3088132CBD516E17 CRC64;
MVNWSPTLQT AVSDLEVEYS EESGYLYHIR YRVAGGSRND WLTVATTRPE TLFGDVALAV
NPEDDRYSKY IGQMAIVPQT FGRHVPIISD KHVDKEFGTG VLKISPGHDH NDYLLARKLG
LPILNVMNKD GTLNEVAGLY SGLDRFEARK KLWAELEETG LAVKKEPHTL RVPRSQRGGE
IIEPLVSKQW FVSMEPLAEK ALQAVEKGEL KIIPERFEKI YNHWLSNIKD WCISRQLWWG
HRIPVWYIVG KDKEEDYIVA RNADEALGKA HKKYGKDVEI YQDPDVLDTW FSSALWPFST
LGWPDLSAED FKRFYPTTML ETGHDILFFW VARMVMMGIE FTGKAPFSYI YLHGLIRDSQ
GRKMSKTLGN VIDPLDTIKE FGTDALRFTV ALGTAGQDLN LSTERLTSNK AFTNKLWNAG
KFVLQNLPKE GDILAWENIL SYKFDTGDSV LNLPLPERWV ISKLHLLIES VSASYDKFFF
GEVGRETYDF FWADFADWYI EASKGRLYNS GNDGNSVTFM AQAVLLYTFE NILKVLHPFM
PFVTEELWQA LPNRKHALIV TPWPETQLPR CTSSIKKFEN LQTLVRAIRN TRAEYSVEPA
KRISASVVAS NEVIEYIAEE KEVLALLSRL DLQNLHFMNS SPGNADQSVH LVAGEGLEAY
LPLADMVDIS AEVQRLSKRL VKMQKEYDGM LAKLNSPKFV EKAPEEVVRA VREKATEAEE
KITLTKNRLE FLNSNVLVSK
//
