ID A0A9J6E9Y0_RHIMP Unreviewed; 229 AA.
AC A0A9J6E9Y0;
DT 28-JUN-2023, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2023, sequence version 1.
DT 18-JUN-2025, entry version 12.
DE RecName: Full=N-terminal E2 ubiquitin-conjugating enzyme {ECO:0000256|ARBA:ARBA00042168};
DE EC=2.3.2.23 {ECO:0000256|ARBA:ARBA00012486};
DE EC=2.3.2.25 {ECO:0000256|ARBA:ARBA00039075};
GN ORFNames=HPB51_012225 {ECO:0000313|EMBL:KAH8030885.1};
OS Rhipicephalus microplus (Cattle tick) (Boophilus microplus).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Rhipicephalinae;
OC Rhipicephalus; Boophilus.
OX NCBI_TaxID=6941 {ECO:0000313|EMBL:KAH8030885.1, ECO:0000313|Proteomes:UP000821866};
RN [1] {ECO:0000313|EMBL:KAH8030885.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Rmic-2018 {ECO:0000313|EMBL:KAH8030885.1};
RX PubMed=32814014;
RG Tick Genome and Microbiome Consortium (TIGMIC);
RA Jia N., Wang J., Shi W., Du L., Sun Y., Zhan W., Jiang J.F., Wang Q.,
RA Zhang B., Ji P., Bell-Sakyi L., Cui X.M., Yuan T.T., Jiang B.G., Yang W.F.,
RA Lam T.T., Chang Q.C., Ding S.J., Wang X.J., Zhu J.G., Ruan X.D., Zhao L.,
RA Wei J.T., Ye R.Z., Que T.C., Du C.H., Zhou Y.H., Cheng J.X., Dai P.F.,
RA Guo W.B., Han X.H., Huang E.J., Li L.F., Wei W., Gao Y.C., Liu J.Z.,
RA Shao H.Z., Wang X., Wang C.C., Yang T.C., Huo Q.B., Li W., Chen H.Y.,
RA Chen S.E., Zhou L.G., Ni X.B., Tian J.H., Sheng Y., Liu T., Pan Y.S.,
RA Xia L.Y., Li J., Zhao F., Cao W.C.;
RT "Large-Scale Comparative Analyses of Tick Genomes Elucidate Their Genetic
RT Diversity and Vector Capacities.";
RL Cell 182:1328-1340(2020).
RN [2] {ECO:0000313|EMBL:KAH8030885.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Rmic-2018 {ECO:0000313|EMBL:KAH8030885.1};
RC TISSUE=Larvae {ECO:0000313|EMBL:KAH8030885.1};
RA Jia N., Wang J., Shi W., Du L., Sun Y., Zhan W., Jiang J., Wang Q.,
RA Zhang B., Ji P., Sakyi L.B., Cui X., Yuan T., Jiang B., Yang W.,
RA Lam T.T.-Y., Chang Q., Ding S., Wang X., Zhu J., Ruan X., Zhao L., Wei J.,
RA Que T., Du C., Cheng J., Dai P., Han X., Huang E., Gao Y., Liu J., Shao H.,
RA Ye R., Li L., Wei W., Wang X., Wang C., Huo Q., Li W., Guo W., Chen H.,
RA Chen S., Zhou L., Zhou L., Ni X., Tian J., Zhou Y., Sheng Y., Liu T.,
RA Pan Y., Xia L., Li J., Zhao F., Cao W.;
RL Submitted (SEP-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000256|ARBA:ARBA00000485};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating
CC enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein].;
CC EC=2.3.2.25; Evidence={ECO:0000256|ARBA:ARBA00035805};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAH8030885.1}.
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DR EMBL; JABSTU010000005; KAH8030885.1; -; Genomic_DNA.
DR VEuPathDB; VectorBase:LOC119163295; -.
DR Proteomes; UP000821866; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR CDD; cd23808; UBCc_UBE2W; 1.
DR FunFam; 3.10.110.10:FF:000022; Ubiquitin-conjugating enzyme E2 W; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR InterPro; IPR009057; Homeodomain-like_sf.
DR InterPro; IPR007889; HTH_Psq.
DR InterPro; IPR050113; Ub_conjugating_enzyme.
DR InterPro; IPR000608; UBQ-conjugat_E2_core.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR PANTHER; PTHR24067; UBIQUITIN-CONJUGATING ENZYME E2; 1.
DR Pfam; PF04218; CENP-B_N; 1.
DR Pfam; PF00179; UQ_con; 1.
DR SMART; SM00212; UBCc; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR SUPFAM; SSF54495; UBC-like; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000821866};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786}.
FT DOMAIN 22..178
FT /note="UBC core"
FT /evidence="ECO:0000259|PROSITE:PS50127"
SQ SEQUENCE 229 AA; 26453 MW; 24D35151052906D8 CRC64;
MLPRFRDDGN RDSKIRHPVF IFWQKRLQKE LLSMQKEPPP GVNVDIDNVG SNLTQWIVDM
EGAKGTLYEG EKFQLLFKFS SKYPFDSPEV TFLGPNIPVH PHVYSNGHIC LSILTDDWSP
ALSVQSVCLS IISMLSSCKE KKRPPDNSLY VKTCNKNPKK TKWWYHELDR NDLSNTEIVK
KFNIPKSTLL RILKNKGKIE EAAKLGYFTT DRMRMRTAAC KEIEDALFL
//
