ID A0A9P0AQ51_BRAAE Unreviewed; 3360 AA.
AC A0A9P0AQ51;
DT 13-SEP-2023, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 1.
DT 18-JUN-2025, entry version 10.
DE RecName: Full=non-specific serine/threonine protein kinase {ECO:0000256|ARBA:ARBA00012513};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN ORFNames=MELIAE_LOCUS987 {ECO:0000313|EMBL:CAH0546905.1};
OS Brassicogethes aeneus (Rape pollen beetle) (Meligethes aeneus).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia; Nitidulidae;
OC Meligethinae; Brassicogethes.
OX NCBI_TaxID=1431903 {ECO:0000313|EMBL:CAH0546905.1, ECO:0000313|Proteomes:UP001154078};
RN [1] {ECO:0000313|EMBL:CAH0546905.1}
RP NUCLEOTIDE SEQUENCE.
RA King R.;
RL Submitted (DEC-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP +
CC H(+); Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00048679};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] +
CC ADP + H(+); Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00047899};
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family.
CC {ECO:0000256|ARBA:ARBA00011031}.
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DR EMBL; OV121132; CAH0546905.1; -; Genomic_DNA.
DR OrthoDB; 10065496at2759; -.
DR Proteomes; UP001154078; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:TreeGrafter.
DR GO; GO:0005634; C:nucleus; IEA:TreeGrafter.
DR GO; GO:0031931; C:TORC1 complex; IEA:TreeGrafter.
DR GO; GO:0031932; C:TORC2 complex; IEA:TreeGrafter.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0016242; P:negative regulation of macroautophagy; IEA:TreeGrafter.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR GO; GO:0031929; P:TOR signaling; IEA:TreeGrafter.
DR CDD; cd05170; PIKKc_SMG1; 1.
DR FunFam; 3.30.1010.10:FF:000010; serine/threonine-protein kinase SMG1 isoform X1; 1.
DR FunFam; 1.10.1070.11:FF:000008; serine/threonine-protein kinase SMG1 isoform X2; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR Gene3D; 3.30.1010.10; Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR050517; DDR_Repair_Kinase.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR014009; PIK_FAT.
DR InterPro; IPR031559; SMG1.
DR InterPro; IPR039414; SMG1_PIKKc.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF119; SERINE_THREONINE-PROTEIN KINASE SMG1; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR Pfam; PF15785; SMG1; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SMART; SM01345; Rapamycin_bind; 1.
DR SUPFAM; SSF48371; ARM repeat; 2.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nonsense-mediated mRNA decay {ECO:0000256|ARBA:ARBA00023161};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP001154078};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1138..1731
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 1975..2312
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 3321..3360
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
FT REGION 1..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..26
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3360 AA; 385804 MW; 773E71AE66F02EFB CRC64;
MISNSSSSYK HKASKNHDFD YDHSDENYDN NSVDDEAVES NHKYNVYSRD GRYRGGIGRA
KSTEFRSSNR LHDRRGGYRG RGGSSRRGDR DYSDNLYSTR KYDLRNSDSG GNYRGKFYEP
EKKVDFKYSL PEDTRISKLL RRLSSEVDQD NSLAISKKLL EVLLIPDNAI YVRKAFHILG
ESMYDIVHVS PGPLAKQQAA RALGRMGYIM GQENDFERYQ HWLFSKMVSS HEEIQILLMK
SFKETFSMEA KKPVLENHAD NLINSLVATI ETTENTEIFK AILDTLMSFV ELYPRNFYDQ
FRDTIDLLFG WHVDHTQSLS NIEFISRNLL RTSHHFKYNL DFSVSLIENF LEDIANYSTQ
IEHVTVLILA LNTILKCLDT DFYPVNNKFG SVEFVNKCLW KIANVFHDFL KEGVQDNLII
AANECISLLL AAIENKSQKL TTKIFELTDD ELALIDDFSD ASIVSLLLFI SKTVKELSTN
LPIELIEKLI GPESKVLYLR HSPVKNIQNA VICVYQSLLN VKNVSLLQEA YRYVLGDLEV
AFRQIVPNLE FLTQNNPFSE RKINKSENFE LTVLFLLRCL SQLANASSII VMWALKPTIL
ELVGVNLQPY NPLLAKNTPS LQYSLLYLLY SHCKCYNHFI SNSSLVTNKK ELPNMMKLFT
ISDGLNMNDV PNTSPNSGNF AIILNILYKT LNTTTSCEVK LLLLQWMNDI LINSENYLEN
LYDNEQFQQI TNILVKCGYK FHPKIAEMVC ENLDKLLSNK QLSWTNIFLS NVCDLCKLHL
NSNHQELRQC YGNLVSNIPW DIAIVELNKK NTGYTKKIRR MQSKDYNNYM LYMAQHLHLN
GNIEGEIYSL QFKAFMDYLL NNEDYPHHWL QELFACCWTY EGQMNMELFY ELAINSRQVL
SNWVTLEAAQ FCVNYKLRTP LGKPNETFTK IEGALNQLGN DLIALKKTGK SDTANTDADR
VKMLLQFVEH LEKSIYNASE GTALAMPQPT KVVKSFFIAN TNTCNEWLSR IRMVVIHIAL
HSGESNIALR HGQSLLRDFV NSGKSNSPEF EKVLIFVTIA FLNLKETEAL YGVYAWCKNM
FAKRLTWIKL AAEQASKKYE IALEGYLKLL EENQNPDEKN KPDCLPLEPD TLNFIQDQIV
VCYKELNNWA DLVKWHEQDK QENIENGRKY WFNSTDWDCN KSLANLDISN HAFKSLSTWE
YKEENKSWSV YETLSAVETN LFNVAVNITS KTVEDSTTKL NTNISTIQRS IQEHLNSAPT
EFLQNFSLLL YVANGLKNVA NNSPAHTVFL VSENFENEIE KVDSSVLRKI LWWSEYFGAV
QNQGFNIFCS NLRLDIIKRA RKEKNHKLAH LHLTKFFRDK DVQLENFEYG SIANTFIEKV
GDIQIWSVDV AKAASEIIKL SYIQEENRNH TFNLCAATST AISKYAELFG GMELRKISSK
ILIKLAAWLQ INENISLTEM NSPLGKLIMV LPEIGMMENI AANIIPLNEM AIGKLLQFSV
HHCTTLAKSW NAFGTWCYRW GKKIVDHSSD IKNNLTDEQC LEIKKLLPSD ATEEDCEKIM
LIISQTRSII DEEDIDSNEI NTSEMIQGQL QCVDVLKYAN DDVLHGLVQI WRNTQKRIYA
YYFLSADAYF KYLHLASNLE NITKSTECYT ITVTLRLLRL IVKYALELQN ILEEGLQTTP
TQPWKVIIPQ LFSRLNHPEG YVRHRVSDLL CRVAEDAPQL ITFPAVVGAL EGGLKFEFSE
ITLPKDCLSQ NNDRIEDNEL NEDDDYDSDN EESTNTLQTC FKTMVDTLSK QDSETITQVQ
TLVKELRRIT LLWDELWLGT LAQHQTEISK RQQQLEFEIE KVNENTNLNS EEKISLIAEK
HRIIIKPIIF ILEQLLDVTS VDAETPHEKE FQEKYMDDIK SVIAKLKNPE TPEKPQESLL
PLKTLQKKFQ QKFHKRASYS LKMQDISPIL HGIKDTVIAM PGIATTAKNI TIAYVSNVVS
ILPTKTKPKK LIFYGSDGQT YTYLFKGLED LHLDERIMQF LSIANTMMAQ NTDPTGHNLY
RARHYSVIPL GPRSGLISWV DGTTPAFALY KRWQQREIAK PSTKNAVNTT TSVLRPSELF
YNKLNPLLQE HGVKNPENRK EWPLAVLKQV LTELMSETPS DLLSKELWCN SISAGAWWQI
VKRYSYSVAV MSIIGYIIGL GDRHLDNMLV DLNTGDVVHI DYNVCFEKGK TLRVPEKVPF
RLTPNIKEAL GATGVEGIFR LACENVLKTM KKGRETLLTL LEAFVYDPLI DWTVGGEVLA
GTTFGGVATT GTSRQSKKDL EREVTLSMFN VRCTEMRAEW NENKDEVLSE LPKLEASLNH
WLSIEKVIAK SDDLLQDLHQ QLALVKEAEA HGANKHPLYK LPMKYAAYHK SQSAMNLAKK
DLETIITDYQ RHLLVYDEAL KSFEKGTSNA LLINISSNGC DTNIFDLVRE FLHNAGKDDV
ILQCQQSETE MDELCQNINI FSKKCLQHLQ NYYSILSTSP KSYLDNHRLK LFVQWASLLL
EKRNIESCEY VFENFNVLLE NNLSTNVFKF AIGLDSLYQE TVGHVNKLFE ELTKLSTKEP
TIVLEKLYSN AKLSISSFLA SEKGAASAFE FVIASELVLL NRNFITLEIA AHRSDKVLIK
LQTREGDWFL DDLVMNSMRA VEMVNTLLLR QTTDDRKMLR VLSGVKIANN IYQGLFDLQS
NFHTIILPES MKKIQSEEPT VLQMINDLNE LISSLDLSIP ELIEQLGKIL QSILMQMDVN
KSYDYILEMV LNIRLKFQTL IGLQTENLSQ GKMLLMGFNG LFEKLSQEMN NLVSTLSDLE
TPKSWKKIDQ VKEAKSISAH IFNPDVRKIL DDIFLLKRIQ TISDFLRICQ DMCQSFKKNA
NLVIYNDEQL AKPVRQFIAD FISSQLLGIT TETIAYTVCC ILQNLGIDLT LEIEQRDIGA
EHKVPLDEIC HKAWNVCLKK GIFTHNVWSQ AQSLENNLKN AWEKIKNPKK LEQKLAQLQS
TAIRLQNQLM VHNFMYDVHG INSVRNKLLA DCHKELLALQ TVLTKLAEAR EKQQILIDNA
YQRLNWAKGA NPNVGEISKA FENAVQLRDN QLNIEQKAAA NILSIGNSIL QYEILRNNNV
ESNAYNNAFS DSFENWRTAC QYGNERIESM SHTEECIMNL LSTELLNDPK WLNVISDQIT
KKISFEQKKL TEAKTFIVSE RLNLQSIIEN LKLIYGVQGR LMSDVKNLIK AMTKIEDYSV
QTHYFIEEYR QYIEHFNLLF AAFKKDVRME EINDAIKHVE FIKTHTEHVY DMLFDLEVKK
GRTSLVRRDC LLNLKMPLKQ DSTTKGQQRN AYAVGVDYVI KEATSLENLA LLYEGWTPWV
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