ID A0A9P6I7Y1_9PEZI Unreviewed; 418 AA.
AC A0A9P6I7Y1;
DT 13-SEP-2023, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 1.
DT 28-JAN-2026, entry version 10.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Name=NCS2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Synonyms=CTU2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN ORFNames=CkaCkLH20_07012 {ECO:0000313|EMBL:KAF9875631.1};
OS Colletotrichum karsti.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum;
OC Colletotrichum boninense species complex.
OX NCBI_TaxID=1095194 {ECO:0000313|EMBL:KAF9875631.1, ECO:0000313|Proteomes:UP000781932};
RN [1] {ECO:0000313|EMBL:KAF9875631.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CkLH20 {ECO:0000313|EMBL:KAF9875631.1};
RA He L.;
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KAF9875631.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CkLH20 {ECO:0000313|EMBL:KAF9875631.1};
RA Li H.;
RT "Whole genome sequencing of Colletotrichum sp.";
RL Submitted (NOV-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with NCS6 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC Prior mcm(5) tRNA modification by the elongator complex is required for
CC 2-thiolation. May also be involved in protein urmylation.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF9875631.1}.
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DR EMBL; JAATWM020000021; KAF9875631.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A9P6I7Y1; -.
DR OrthoDB; 25129at2759; -.
DR Proteomes; UP000781932; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP000781932};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
SQ SEQUENCE 418 AA; 46162 MW; 93B9A552019C000F CRC64;
MSGNNESRPC RRCKTPTTLT VRKDPLCESC YEQHISQRVA KALGKPYKDL KDKIGNAANA
KAKYLLGLSF GHSSAAMVQV LDKNLQSLHE RKLRVAYEVH VVHVDTDLSD TCTSSGGSEA
SELLEKHKAR FPNLPMRCVP LSDALKLDTI DWSVLPALQK DLEPAQQLRA MLEALPSVTS
RVDIMRLLVR HLLLSLALGN ECHALLLGCT TTALAELTLA ETAKGRGFAI PWQVNDGPVP
ITTFPRCSEH AKDDQMVNET QTTTFPVYYL LRDVFKREIV TYTGLVQPTI RDVIRDAGPS
SNTIVSHKEL SIEDVMARYF EEVEENYPSV VANVVRTSGK LTRVNNGNAC AVCGMDLDEQ
GDARWKGEIG EDPDDTKRKK TVGRNLDLCS AAFMNPPPIP SAKSIPGHDK PVQSIQLF
//
