ID A0A9P9E7I4_9HYPO Unreviewed; 397 AA.
AC A0A9P9E7I4;
DT 13-SEP-2023, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 1.
DT 28-JAN-2026, entry version 10.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Name=NCS2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Synonyms=CTU2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN ORFNames=B0J13DRAFT_597929 {ECO:0000313|EMBL:KAH7131396.1};
OS Dactylonectria estremocensis.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Dactylonectria.
OX NCBI_TaxID=1079267 {ECO:0000313|EMBL:KAH7131396.1, ECO:0000313|Proteomes:UP000717696};
RN [1] {ECO:0000313|EMBL:KAH7131396.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=MPI-CAGE-AT-0021 {ECO:0000313|EMBL:KAH7131396.1};
RA Mesny F., Miyauchi S., Thiergart T., Pickel B., Atanasova L., Karlsson M.,
RA Huettel B., Barry K.W., Haridas S., Chen C., Bauer D., Andreopoulos W.,
RA Pangilinan J., LaButti K., Riley R., Lipzen A., Clum A., Drula E.,
RA Henrissat B., Kohler A., Grigoriev I.V., Martin F.M., Hacquard S.;
RT "Genetic determinants of endophytism in the Arabidopsis root mycobiome.";
RL Nat. Commun. 0:0-0(2021).
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with NCS6 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC Prior mcm(5) tRNA modification by the elongator complex is required for
CC 2-thiolation. May also be involved in protein urmylation.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAH7131396.1}.
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DR EMBL; JAGMUU010000019; KAH7131396.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A9P9E7I4; -.
DR OrthoDB; 25129at2759; -.
DR Proteomes; UP000717696; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP000717696};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
SQ SEQUENCE 397 AA; 43672 MW; ACE291866BDDBDFC CRC64;
MPGSETSRPC KRCKAEDAQF RLRTDPTCRD CYVQYVSLKM SKRLGQMHRD IRVSTLPTAR
RYMAGLSFGP SSSVLVQLLD DTGRRLSLKK SASTFNPIAV HVDTDLSAGE ESPARKLIEE
YRRRFQHVEF ECVPLSDVLT VRSIDWSALP LPVEDDGKTE PSVRLQRLFD ALPTVTSRAD
VLRQFVRHLL LHRGLESFSS ALVFGHSTSA LAALTLSEVA NGRGFAVPWQ VNDGMCTISK
YPAPGASGND PDATAPAQEA DRTEFPVYYP MREVFRNEIL QYVDLVPSLS GLVPADQMAI
GGRNNVVSHK DLSIEEVMAR YFDSVEDSYS GIVANVVRTT GKLERIAGGE FCGSCGMTVD
DLGDSRWAGE IGDDPVGAGN SRETGRLCYG CKRSIYG
//
