ID A0A9Q0SR96_SALPP Unreviewed; 331 AA.
AC A0A9Q0SR96;
DT 13-SEP-2023, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 1.
DT 10-JUN-2026, entry version 12.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN ORFNames=OIU79_016248 {ECO:0000313|EMBL:KAJ6686430.1};
OS Salix purpurea (Purple osier willow).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Salicaceae; Saliceae; Salix.
OX NCBI_TaxID=77065 {ECO:0000313|EMBL:KAJ6686430.1, ECO:0000313|Proteomes:UP001151532};
RN [1] {ECO:0000313|EMBL:KAJ6686430.1, ECO:0000313|Proteomes:UP001151532}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 94001 {ECO:0000313|Proteomes:UP001151532};
RC TISSUE=Shoot tip {ECO:0000313|EMBL:KAJ6686430.1};
RX PubMed=36769224; DOI=10.3390/ijms24032904;
RA Hyden B., Feng K., Yates T.B., Jawdy S., Cereghino C., Smart L.B.,
RA Muchero W.;
RT "De Novo Assembly and Annotation of 11 Diverse Shrub Willow (Salix) Genomes
RT Reveals Novel Gene Organization in Sex-Linked Regions.";
RL Int. J. Mol. Sci. 24:2904-2904(2023).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the SINA (Seven in absentia) family.
CC {ECO:0000256|ARBA:ARBA00009119}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAJ6686430.1}.
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DR EMBL; JAPFFK010000019; KAJ6686430.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A9Q0SR96; -.
DR OrthoDB; 941555at2759; -.
DR Proteomes; UP001151532; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR CDD; cd16571; RING-HC_SIAHs; 1.
DR CDD; cd03829; Sina; 1.
DR FunFam; 3.30.40.10:FF:000041; E3 ubiquitin-protein ligase SINAT3; 1.
DR FunFam; 3.30.40.10:FF:000483; E3 ubiquitin-protein ligase SINAT3; 1.
DR FunFam; 2.60.210.10:FF:000004; E3 ubiquitin-protein ligase SINAT5-like; 1.
DR Gene3D; 2.60.210.10; Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 2.
DR InterPro; IPR018121; 7-in-absentia-prot_TRAF-dom.
DR InterPro; IPR052088; E3_ubiquitin-ligase_SINA.
DR InterPro; IPR049548; Sina-like_RING.
DR InterPro; IPR008974; TRAF-like.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR013010; Znf_SIAH.
DR PANTHER; PTHR10315; E3 UBIQUITIN PROTEIN LIGASE SIAH; 1.
DR PANTHER; PTHR10315:SF133; RING-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF21362; Sina_RING; 1.
DR Pfam; PF03145; Sina_TRAF; 1.
DR Pfam; PF21361; Sina_ZnF; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR SUPFAM; SSF49599; TRAF domain-like; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
DR PROSITE; PS51081; ZF_SIAH; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP001151532};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00455}.
FT DOMAIN 69..105
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT DOMAIN 122..182
FT /note="SIAH-type"
FT /evidence="ECO:0000259|PROSITE:PS51081"
FT REGION 33..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..53
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 331 AA; 37629 MW; D6C5FB98B8829F85 CRC64;
MELGRVECVQ SYEIVDSEEI HLLPDLLRHF SSVSKPHPTT TNNNNNSNNN SNTVASAVHT
TSVHELLECP VCTNSMYPPI HQCHNGHTLC STCKTRVQNR CPTCRQELGD IRCLALEKVA
ESLELPCKYM SLGCPEIFPY YSKLKHETLC NFRPYSCPYA GSECAIVGDI PFLVAHLRDD
HKVDMHSGCT FNHRYVKSNP REVENATWML TVFHCFGQYF CLHFEAFQLG MAPVYMAFLR
FMGDEAEARN YSYSLEVGGN GRKLIWEGTP RSIRDSHRKV RDSHDGLIIQ RNMALFFSGG
DRKELKLRVT GRIWKEQQNP EGGACIPNLC S
//
