ID A0A9Q9IH14_9ACTN Unreviewed; 305 AA.
AC A0A9Q9IH14;
DT 13-SEP-2023, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 1.
DT 28-JAN-2026, entry version 11.
DE RecName: Full=Methylenetetrahydrofolate reductase {ECO:0000256|RuleBase:RU003862};
DE EC=1.5.1.54 {ECO:0000256|RuleBase:RU003862};
GN Name=metF {ECO:0000313|EMBL:UWZ53239.1};
GN ORFNames=Daura_42855 {ECO:0000313|EMBL:UWZ53239.1};
OS Dactylosporangium aurantiacum.
OC Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Dactylosporangium.
OX NCBI_TaxID=35754 {ECO:0000313|EMBL:UWZ53239.1, ECO:0000313|Proteomes:UP001058003};
RN [1] {ECO:0000313|EMBL:UWZ53239.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NRRL B-8018 {ECO:0000313|EMBL:UWZ53239.1};
RA Hartkoorn R.C., Beaudoing E., Hot D.;
RT "Dactylosporangium aurantiacum NRRL B-8018 full assembly.";
RL Submitted (APR-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-
CC methylene-5,6,7,8-tetrahydrofolate + NADH + H(+);
CC Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.54;
CC Evidence={ECO:0000256|ARBA:ARBA00048628};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:19823;
CC Evidence={ECO:0000256|ARBA:ARBA00048628};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|RuleBase:RU003862};
CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC pathway. {ECO:0000256|ARBA:ARBA00034478}.
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU003862}.
CC -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC {ECO:0000256|ARBA:ARBA00006743, ECO:0000256|RuleBase:RU003862}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP073767; UWZ53239.1; -; Genomic_DNA.
DR RefSeq; WP_033362560.1; NZ_CP073767.1.
DR AlphaFoldDB; A0A9Q9IH14; -.
DR KEGG; daur:Daura_42855; -.
DR OrthoDB; 9812555at2; -.
DR Proteomes; UP001058003; Chromosome.
DR GO; GO:0005829; C:cytosol; IEA:InterPro.
DR GO; GO:0071949; F:FAD binding; IEA:TreeGrafter.
DR GO; GO:0106312; F:methylenetetrahydrofolate reductase (NADH) activity; IEA:UniProtKB-EC.
DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:TreeGrafter.
DR CDD; cd00537; MTHFR; 1.
DR Gene3D; 3.20.20.220; -; 1.
DR InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR InterPro; IPR003171; Mehydrof_redctse-like.
DR InterPro; IPR004620; MTHF_reductase_bac.
DR NCBIfam; TIGR00676; fadh2; 1.
DR PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH); 1.
DR Pfam; PF02219; MTHFR; 1.
DR SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU003862};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|RuleBase:RU003862};
KW Methionine biosynthesis {ECO:0000256|ARBA:ARBA00023167};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU003862};
KW Reference proteome {ECO:0000313|Proteomes:UP001058003}.
SQ SEQUENCE 305 AA; 32770 MW; F6E9424CC5141D5B CRC64;
MASGLPSVMG GGPAAIGDLV AGAAPTFSFE FFPPKTPEGE RQLWQAIREL EPLRPSFVSI
TYGAGGTTRD TTVAVTERVA TETTLLPMAH LTAVNHSVAE LRNVIGQLAG AGIRNFLLVR
GDPPGDPMGE WVQHPEGVLY AEELVRLVRE SGDFSVGVAA FPYKHPRSPD IESDTANFVR
KCRAGADFAI TQMFFDADDY LRLRDRVAAA GCDTPIVAGV MPVTAMGTIA RSQQLSGAPF
PPALAERFDR VADDPQAVRR LGIDLASEMC ERLLDEGVPG IHFITLNRST ATREVWQQLG
VGARV
//
