ID A0A9W6JH44_9HYPH Unreviewed; 650 AA.
AC A0A9W6JH44;
DT 08-NOV-2023, integrated into UniProtKB/TrEMBL.
DT 08-NOV-2023, sequence version 1.
DT 28-JAN-2026, entry version 12.
DE RecName: Full=Selenocysteine-specific elongation factor {ECO:0000256|ARBA:ARBA00015953};
DE AltName: Full=SelB translation factor {ECO:0000256|ARBA:ARBA00031615};
GN Name=selB {ECO:0000313|EMBL:GLK75649.1};
GN ORFNames=GCM10008171_09030 {ECO:0000313|EMBL:GLK75649.1};
OS Methylopila jiangsuensis.
OC Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC Hyphomicrobiales; Methylopilaceae; Methylopila.
OX NCBI_TaxID=586230 {ECO:0000313|EMBL:GLK75649.1, ECO:0000313|Proteomes:UP001143364};
RN [1] {ECO:0000313|EMBL:GLK75649.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=VKM B-2555 {ECO:0000313|EMBL:GLK75649.1};
RX PubMed=25193709;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Walter F., Albersmeier A., Kalinowski J., Ruckert C.;
RT "Complete genome sequence of Corynebacterium casei LMG S-19264T (=DSM
RT 44701T), isolated from a smear-ripened cheese.";
RL Int. J. Syst. Evol. Microbiol. 189:76-77(2014).
RN [2] {ECO:0000313|EMBL:GLK75649.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=VKM B-2555 {ECO:0000313|EMBL:GLK75649.1};
RA Sun Q., Evtushenko L.;
RL Submitted (JAN-2023) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Translation factor necessary for the incorporation of
CC selenocysteine into proteins. It probably replaces EF-Tu for the
CC insertion of selenocysteine directed by the UGA codon. SelB binds GTP
CC and GDP. {ECO:0000256|ARBA:ARBA00025526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GLK75649.1}.
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DR EMBL; BSFK01000005; GLK75649.1; -; Genomic_DNA.
DR RefSeq; WP_271203590.1; NZ_BSFK01000005.1.
DR AlphaFoldDB; A0A9W6JH44; -.
DR Proteomes; UP001143364; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0004020; F:adenylylsulfate kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:InterPro.
DR GO; GO:0001514; P:selenocysteine incorporation; IEA:InterPro.
DR CDD; cd04171; SelB; 1.
DR CDD; cd03696; SelB_II; 1.
DR CDD; cd15491; selB_III; 1.
DR FunFam; 3.40.50.300:FF:001064; Selenocysteine-specific translation elongation factor; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 3.
DR InterPro; IPR057335; Beta-barrel_SelB.
DR InterPro; IPR050055; EF-Tu_GTPase.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR015190; Elong_fac_SelB-wing-hlx_typ-2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015191; SelB_WHD4.
DR InterPro; IPR005225; Small_GTP-bd.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004535; Transl_elong_SelB.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR048931; WHD_2nd_SelB_bact.
DR NCBIfam; TIGR00475; selB; 1.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43721:SF22; ELONGATION FACTOR TU, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43721; ELONGATION FACTOR TU-RELATED; 1.
DR Pfam; PF25461; Beta-barrel_SelB; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF09106; WHD_2nd_SelB; 1.
DR Pfam; PF21214; WHD_2nd_SelB_bact; 1.
DR Pfam; PF09107; WHD_3rd_SelB; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 3.
DR PROSITE; PS51722; G_TR_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP001143364}.
FT DOMAIN 1..173
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 650 AA; 69235 MW; FB544EAE33349555 CRC64;
MIVGTAGHID HGKSALVKAL TGVETDRLKE EKARGISIDL GFAYSPRADG QVLGFVDVPG
HERFVRNMLA GASGIDLVLL VVAADDGPMP QTREHLAIVD LLGVRRGLVA LTKTDLVDAA
RLAEAESEVV ALLGGTGLEG ADILPVSAVT GDGVAALSEK LDALRAQTRE RPAEGRFRLA
VDRCFTLQGA GTVVTGAVRD GVVRVGDPVI VSPSGLTARV RAIHAQNRAA EEGRPGERCA
LNLAGPGVAK DAIRRGDVVC AAELHAPTER IDVSLRLLSG EPRPMATWTP ARLHHGAAEI
AARVVPLDGD HIAPGAEVRA QLVLEQPLAA AALDRFVLRD TSGQRTVAGG RILDLRAPAR
RRRSPERLAQ LAALAKDDPA EAVAELLAVP PGTVDLDGFA RDRNLGPGAA ARLAETLDLV
RLAGPRGAAA VVTRDVWDAF AADLAARLAV FHADSPDLQG VGAERLRLMV EPRLAAPAFL
GALARLQREG AIAVDGAWVR RPEHTARLAP ADEALWAEIG PRLGGATRFR PPRVRDLAHE
LGEDEADIRR ALKLVSRLGQ ADEIAHDHFF PREVTSELVE VACAAAAADP RGVLTAAAFR
DRLDNGRKVA IQILEFFDRH GVTIRRGDER RINSRRRDLF RRAPVTEQEA
//
