ID A0A9W9QR22_PENBR Unreviewed; 501 AA.
AC A0A9W9QR22;
DT 08-NOV-2023, integrated into UniProtKB/TrEMBL.
DT 08-NOV-2023, sequence version 1.
DT 28-JAN-2026, entry version 10.
DE RecName: Full=DNA primase large subunit {ECO:0000256|PIRNR:PIRNR009449};
GN ORFNames=N7452_006591 {ECO:0000313|EMBL:KAJ5339863.1}, N7541_011816
GN {ECO:0000313|EMBL:KAJ5342692.1};
OS Penicillium brevicompactum.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX NCBI_TaxID=5074 {ECO:0000313|EMBL:KAJ5342692.1, ECO:0000313|Proteomes:UP001148299};
RN [1] {ECO:0000313|EMBL:KAJ5342692.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=IBT 35673 {ECO:0000313|EMBL:KAJ5339863.1}, and IBT 35675
RC {ECO:0000313|EMBL:KAJ5342692.1};
RA Petersen C.;
RL Submitted (DEC-2022) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KAJ5342692.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=IBT 35673 {ECO:0000313|EMBL:KAJ5339863.1}, and IBT 35675
RC {ECO:0000313|EMBL:KAJ5342692.1};
RX PubMed=36726175;
RA Petersen C., Sorensen T., Nielsen M.R., Sondergaard T.E., Sorensen J.L.,
RA Fitzpatrick D.A., Frisvad J.C., Nielsen K.L.;
RT "Comparative genomic study of the Penicillium genus elucidates a diverse
RT pangenome and 15 lateral gene transfer events.";
RL IMA Fungus 14:0-0(2023).
CC -!- FUNCTION: DNA primase is the polymerase that synthesizes small RNA
CC primers for the Okazaki fragments made during discontinuous DNA
CC replication. {ECO:0000256|PIRNR:PIRNR009449}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|PIRNR:PIRNR009449};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000256|PIRNR:PIRNR009449};
CC -!- SIMILARITY: Belongs to the eukaryotic-type primase large subunit
CC family. {ECO:0000256|ARBA:ARBA00010564, ECO:0000256|PIRNR:PIRNR009449}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAJ5342692.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JAPZBQ010000003; KAJ5339863.1; -; Genomic_DNA.
DR EMBL; JAPZBR010000008; KAJ5342692.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A9W9QR22; -.
DR Proteomes; UP001147695; Unassembled WGS sequence.
DR Proteomes; UP001148299; Unassembled WGS sequence.
DR GO; GO:0005658; C:alpha DNA polymerase:primase complex; IEA:TreeGrafter.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006270; P:DNA replication initiation; IEA:TreeGrafter.
DR GO; GO:0006269; P:DNA replication, synthesis of primer; IEA:UniProtKB-KW.
DR CDD; cd07322; PriL_PriS_Eukaryotic; 1.
DR FunFam; 1.20.930.80:FF:000003; DNA primase large subunit; 1.
DR Gene3D; 1.20.930.80; -; 1.
DR InterPro; IPR058560; DNA_primase_C.
DR InterPro; IPR016558; DNA_primase_lsu_euk.
DR InterPro; IPR007238; DNA_primase_lsu_euk/arc.
DR PANTHER; PTHR10537; DNA PRIMASE LARGE SUBUNIT; 1.
DR PANTHER; PTHR10537:SF3; DNA PRIMASE LARGE SUBUNIT; 1.
DR Pfam; PF04104; DNA_primase_lrg; 1.
DR Pfam; PF26466; DNA_primase_lrg_N; 1.
DR PIRSF; PIRSF009449; DNA_primase_large_subunit; 1.
DR SUPFAM; SSF140914; PriB N-terminal domain-like; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|PIRNR:PIRNR009449};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|PIRNR:PIRNR009449};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PIRNR:PIRNR009449};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRNR:PIRNR009449};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRNR:PIRNR009449};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRNR:PIRNR009449};
KW Primosome {ECO:0000256|ARBA:ARBA00022515, ECO:0000256|PIRNR:PIRNR009449};
KW Reference proteome {ECO:0000313|Proteomes:UP001148299}.
FT DOMAIN 298..485
FT /note="DNA primase large subunit C-terminal"
FT /evidence="ECO:0000259|Pfam:PF04104"
FT BINDING 308
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
FT BINDING 391
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
FT BINDING 408
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
FT BINDING 449
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
SQ SEQUENCE 501 AA; 57895 MW; D6623B45B729E4E3 CRC64;
MIRQDVNRID PKRRTIIDPK KRQFAPATFK QQDYPHRMNI YDTPPTAEIT LEQFEQWAID
RLKILAEIEA CSYRNKSPAE TASYITPLLQ KFLPLSSNTS SSSGSADQRL KNERQKDHYS
HFILRLAFSA TEDLRRRFAR AESMLFRFRF QQDDSREKRA FIDSLNLDWE PLGDFEKTEV
AEALMSSTAG LRRVDDETWY KVDWEKVPEL IERRAVYLKK GKAYVPGREQ MSMILAEFTT
RLERALELTS RALPRLDEDD RLTPILNHLS KNFGSADSVY SEGEGYIDGA PITASSIDQL
AQQHFPLCMK SLHVNLRKNN HLKHFGRLQY TLFLKGIGLS LEECILFWRQ SFKGKTDDEF
NSQYKYNIRH AYGEVGGDVN RRGKGYTPYS CQKILGDSNP GIGQTHGCPY RHYSVDNLVG
FLQATGVSDK EVLRGVREDV GKMRYHIACN RVFEATHKNE IKKVKEEGTM SQTDLDTIVH
PNTYFKRSFL LKQMSKAPKD A
//
