ID A0A9X4LJK7_9BURK Unreviewed; 621 AA.
AC A0A9X4LJK7;
DT 08-NOV-2023, integrated into UniProtKB/TrEMBL.
DT 08-NOV-2023, sequence version 1.
DT 28-JAN-2026, entry version 12.
DE RecName: Full=Selenocysteine-specific elongation factor {ECO:0000256|ARBA:ARBA00015953};
DE AltName: Full=SelB translation factor {ECO:0000256|ARBA:ARBA00031615};
GN Name=selB {ECO:0000313|EMBL:MDG0863642.1};
GN ORFNames=EXJ73_14335 {ECO:0000313|EMBL:MDG0863642.1};
OS Pelomonas aquatica.
OC Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
OC Burkholderiales; Sphaerotilaceae; Roseateles.
OX NCBI_TaxID=431058 {ECO:0000313|EMBL:MDG0863642.1, ECO:0000313|Proteomes:UP001152766};
RN [1] {ECO:0000313|EMBL:MDG0863642.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CCUG 52575 {ECO:0000313|EMBL:MDG0863642.1};
RA Gomila M., Lalucat J.;
RT "Draft genome of the type strain Pelomonas aquatica CCUG 52575T.";
RL Submitted (FEB-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Translation factor necessary for the incorporation of
CC selenocysteine into proteins. It probably replaces EF-Tu for the
CC insertion of selenocysteine directed by the UGA codon. SelB binds GTP
CC and GDP. {ECO:0000256|ARBA:ARBA00025526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:MDG0863642.1}.
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DR EMBL; SGUG01000020; MDG0863642.1; -; Genomic_DNA.
DR RefSeq; WP_268149571.1; NZ_JAPPUW010000007.1.
DR AlphaFoldDB; A0A9X4LJK7; -.
DR Proteomes; UP001152766; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0001514; P:selenocysteine incorporation; IEA:InterPro.
DR CDD; cd04171; SelB; 1.
DR CDD; cd15491; selB_III; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 2.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 3.
DR InterPro; IPR057335; Beta-barrel_SelB.
DR InterPro; IPR050055; EF-Tu_GTPase.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR015190; Elong_fac_SelB-wing-hlx_typ-2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015191; SelB_WHD4.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004535; Transl_elong_SelB.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR048931; WHD_2nd_SelB_bact.
DR NCBIfam; TIGR00475; selB; 1.
DR PANTHER; PTHR43721:SF22; ELONGATION FACTOR TU, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43721; ELONGATION FACTOR TU-RELATED; 1.
DR Pfam; PF25461; Beta-barrel_SelB; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF09106; WHD_2nd_SelB; 1.
DR Pfam; PF21214; WHD_2nd_SelB_bact; 1.
DR Pfam; PF09107; WHD_3rd_SelB; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 3.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Elongation factor {ECO:0000313|EMBL:MDG0863642.1};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP001152766}.
FT DOMAIN 1..171
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 621 AA; 65595 MW; 20C19284A24A40A3 CRC64;
MIVGTAGHID HGKTTLVRAL TGVDTDRLPE EKARGISIEL GYAYLDVPEH DERIGFIDVP
GHEKLVHTML AGASGIDFAL LLVAADDGVM PQTREHLAVL SLLGLTRGAA VVTKVDRAEP
GRVAALDDEL RALLAGTGLD AAPVLAVSAL TGQGVAELRE LLFAAARRTP PRSPAGLAAR
LAVDRAFSLD GVGTVVTGTL HAGEIAVGDE LVVQGLHARV RSLHAQNRAV QRAQAGQRCA
VALAGLARAQ AARGQWLTAP AAALATERVD ARLRLWPGEP RPLRSGTRVH LHLGAQGVMA
SIAVLEGDAI EPGASARVQL VCHAAVGAWR GDRLVLRDAG ATRTVAGGVV LDPFAPARYR
RTPERLAELA ACELPTVARR RERLLALSAL GLDLGRFARA EGLAEAQQGW ALGDAASEAL
TQRLLAALAD FHARSPDELG PDGARLRRLV APRLAEAPWL ELLAGLRAAG RVAQRGAFVH
LPEHGVQLSA VERRIAQKIA PLLAAAGADG AWARDLARDC AEPEPLLRAT LARLAQQGEL
HQVVRDLFFD TATMTALAAT TRRLAAGDGS VTAAAFRDAT GLGRKRAIQV MEYFDRIGLL
RRVGDEHRLR ADSALFDEPG S
//
