ID A0AA40D508_9PEZI Unreviewed; 309 AA.
AC A0AA40D508;
DT 24-JAN-2024, integrated into UniProtKB/TrEMBL.
DT 24-JAN-2024, sequence version 1.
DT 18-JUN-2025, entry version 7.
DE RecName: Full=thymidylate synthase {ECO:0000256|ARBA:ARBA00011947};
DE EC=2.1.1.45 {ECO:0000256|ARBA:ARBA00011947};
GN ORFNames=QBC41DRAFT_26454 {ECO:0000313|EMBL:KAK0663395.1};
OS Cercophora samala.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Lasiosphaeriaceae; Cercophora.
OX NCBI_TaxID=330535 {ECO:0000313|EMBL:KAK0663395.1, ECO:0000313|Proteomes:UP001174997};
RN [1] {ECO:0000313|EMBL:KAK0663395.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CBS 307.81 {ECO:0000313|EMBL:KAK0663395.1};
RG Lawrence Berkeley National Laboratory;
RA Hensen N., Bonometti L., Westerberg I., Brannstrom I.O., Guillou S.,
RA Cros-Aarteil S., Calhoun S., Haridas S., Kuo A., Mondo S., Pangilinan J.,
RA Riley R., Labutti K., Andreopoulos B., Lipzen A., Chen C., Yanf M.,
RA Daum C., Ng V., Clum A., Steindorff A., Ohm R., Martin F., Silar P.,
RA Natvig D., Lalanne C., Gautier V., Ament-Velasquez S.L., Kruys A.,
RA Hutchinson M.I., Powell A.J., Barry K., Miller A.N., Grigoriev I.V.,
RA Debuchy R., Gladieux P., Thoren M.H., Johannesson H.;
RT "Genome-scale phylogeny and comparative genomics of the fungal order
RT Sordariales.";
RL Submitted (JUN-2023) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-
CC dihydrofolate + dTMP; Xref=Rhea:RHEA:12104, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:57451, ChEBI:CHEBI:63528, ChEBI:CHEBI:246422;
CC EC=2.1.1.45; Evidence={ECO:0000256|ARBA:ARBA00047344};
CC -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004992}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAK0663395.1}.
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DR EMBL; JAULSY010000132; KAK0663395.1; -; Genomic_DNA.
DR Proteomes; UP001174997; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0005739; C:mitochondrion; IEA:TreeGrafter.
DR GO; GO:0004799; F:thymidylate synthase activity; IEA:InterPro.
DR GO; GO:0006231; P:dTMP biosynthetic process; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd00351; TS_Pyrimidine_HMase; 1.
DR FunFam; 3.30.572.10:FF:000013; Thymidylate synthase; 1.
DR Gene3D; 3.30.572.10; Thymidylate synthase/dCMP hydroxymethylase domain; 1.
DR HAMAP; MF_00008; Thymidy_synth_bact; 1.
DR InterPro; IPR045097; Thymidate_synth/dCMP_Mease.
DR InterPro; IPR023451; Thymidate_synth/dCMP_Mease_dom.
DR InterPro; IPR036926; Thymidate_synth/dCMP_Mease_sf.
DR InterPro; IPR000398; Thymidylate_synthase.
DR InterPro; IPR020940; Thymidylate_synthase_AS.
DR NCBIfam; TIGR03284; thym_sym; 1.
DR PANTHER; PTHR11548:SF2; THYMIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11548; THYMIDYLATE SYNTHASE 1; 1.
DR Pfam; PF00303; Thymidylat_synt; 1.
DR PRINTS; PR00108; THYMDSNTHASE.
DR SUPFAM; SSF55831; Thymidylate synthase/dCMP hydroxymethylase; 1.
DR PROSITE; PS00091; THYMIDYLATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Nucleotide biosynthesis {ECO:0000256|ARBA:ARBA00022727};
KW Reference proteome {ECO:0000313|Proteomes:UP001174997};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 19..309
FT /note="Thymidylate synthase/dCMP hydroxymethylase"
FT /evidence="ECO:0000259|Pfam:PF00303"
FT ACT_SITE 171
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10016"
SQ SEQUENCE 309 AA; 34807 MW; E3EDA8963042D1A3 CRC64;
MSPHFPLSSS RQTTKLPLRR TGTGTYSIFA PTPLKFALSR PDGTPLLPLL TTKRVFTRAV
ILELLWFISG STSSLPLSAQ NVKIWDGNGS RAFLDSLGLT HRPEGDLGPV YGFQWRHFGA
EYKDAETDYT GQGVDQLARI VETLRTNPYD RRLILSAWNP KDMSQMVLPP CHMFAQFYVS
YPRSRQGAEE EEEGKDEVVK GHLHCQLYQR SCDMGLGVPF NIASYALLTH MLAHVCDLVP
GSLTHVMGDA HVYLNHVEAL KTQLEREPRD FPELEINREK GGSIDGWKLE DFEIKGYNPH
KGISMEMSV
//
