ID A0AA97MXL1_9PASS Unreviewed; 679 AA.
AC A0AA97MXL1;
DT 27-MAR-2024, integrated into UniProtKB/TrEMBL.
DT 27-MAR-2024, sequence version 1.
DT 28-JAN-2026, entry version 10.
DE RecName: Full=Nicastrin {ECO:0000256|ARBA:ARBA00015303};
DE Flags: Fragment;
GN Name=Ncstn {ECO:0000313|EMBL:NXE99524.1};
GN ORFNames=MENNOV_R05931 {ECO:0000313|EMBL:NXE99524.1};
OS Menura novaehollandiae (superb lyrebird).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Neoaves; Telluraves; Australaves;
OC Passeriformes; Menuridae; Menura.
OX NCBI_TaxID=47692 {ECO:0000313|EMBL:NXE99524.1};
RN [1] {ECO:0000313|EMBL:NXE99524.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=B10K-CU-030-46 {ECO:0000313|EMBL:NXE99524.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:NXE99524.1};
RA Zhang G.;
RT "Bird 10,000 Genomes (B10K) Project - Family phase.";
RL Submitted (DEC-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Essential subunit of the gamma-secretase complex, an
CC endoprotease complex that catalyzes the intramembrane cleavage of
CC integral membrane proteins such as Notch receptors and APP (amyloid-
CC beta precursor protein). The gamma-secretase complex plays a role in
CC Notch and Wnt signaling cascades and regulation of downstream processes
CC via its role in processing key regulatory proteins, and by regulating
CC cytosolic CTNNB1 levels. {ECO:0000256|ARBA:ARBA00058029}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC {ECO:0000256|ARBA:ARBA00037855}; Single-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00037855}. Endomembrane system
CC {ECO:0000256|ARBA:ARBA00046288}; Single-pass type I membrane protein
CC {ECO:0000256|ARBA:ARBA00046288}. Melanosome
CC {ECO:0000256|ARBA:ARBA00004223}.
CC -!- SIMILARITY: Belongs to the nicastrin family.
CC {ECO:0000256|ARBA:ARBA00007717}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:NXE99524.1}.
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DR EMBL; VWPS01000884; NXE99524.1; -; Genomic_DNA.
DR AlphaFoldDB; A0AA97MXL1; -.
DR Proteomes; UP000521578; Unassembled WGS sequence.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
DR GO; GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-ARBA.
DR GO; GO:0032991; C:protein-containing complex; IEA:UniProtKB-ARBA.
DR GO; GO:0099503; C:secretory vesicle; IEA:UniProtKB-ARBA.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-ARBA.
DR GO; GO:0007220; P:Notch receptor processing; IEA:TreeGrafter.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0016485; P:protein processing; IEA:InterPro.
DR CDD; cd03881; M28_Nicastrin; 1.
DR FunFam; 3.40.630.10:FF:000030; nicastrin; 1.
DR Gene3D; 3.40.630.10; Zn peptidases; 1.
DR InterPro; IPR041084; Ncstrn_small.
DR InterPro; IPR008710; Nicastrin.
DR PANTHER; PTHR21092; NICASTRIN; 1.
DR PANTHER; PTHR21092:SF0; NICASTRIN; 1.
DR Pfam; PF18266; Ncstrn_small; 1.
DR Pfam; PF05450; Nicastrin; 1.
DR SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE 3: Inferred from homology;
KW Cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00023329};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Notch signaling pathway {ECO:0000256|ARBA:ARBA00022976};
KW Reference proteome {ECO:0000313|Proteomes:UP000521578};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 640..660
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 22..195
FT /note="Nicastrin small lobe"
FT /evidence="ECO:0000259|Pfam:PF18266"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:NXE99524.1"
FT NON_TER 679
FT /evidence="ECO:0000313|EMBL:NXE99524.1"
SQ SEQUENCE 679 AA; 75236 MW; 71A091D1762D8EB7 CRC64;
SGSCHGNSVE RKIYIPLNRT APCVRLLNAT HQIGCQSSIS GDTGVIHVVE KEEDLGWVLA
DGPHPPYMIL LDGNLFTRSV LLQLKGTSRV SGLAVAVAKP SPPQGFSPGL KCPNDGFGVY
SKDYGPQFAH CNTTVWNPLG SGLSYEDFDF PIFLLEDTNE TQVIKQCYQA HNVPRDGSGP
EYPLCAMQLF SHMHAVTSTV TCMRRSSLQS TFSINPEIVC DPLLDYNVWS TLQPINSSGK
VDPDKEVIMV ATRIDSHSFF WNVAPGAEGA VSSFVTHLAT AQALHRAPDA QRLPRNVMFT
FFQGETFDYI GSSRMVYDME QEKFPLRLEN IYSFVELNQV ALRNDSILWM HTDPVSRMNE
SVEAQVRNLL DTLRRSSAGA GVTLQEAGFS QPLPPSSFQR FLRARHIPGV VLTDHRTAFH
NRYFQSMYDT PENIGMQYPE GLSPEETLEY VTDTAKALAG VATVLARALY GLAGGANSTS
AIQADPKTVT RMLYGFLIKM NNSWFQSIIK PDLKGILGDV PQHYVAVSSP VNTTYLVQFV
LANLTGSVVN LSKEECQNSE KSPGSDRELY DFSWVQGSLD PNSSSRIPLC VRSTVRLSKA
LSPAFELRQW GSREYSTWTE SRWKDIRARI FLVASRELEI LTLVLGIAIL ILSLLTTYFI
NAKADVLFSI PRDPGALGY
//
