ID A0AAD6NAQ4_PENCN Unreviewed; 501 AA.
AC A0AAD6NAQ4;
DT 29-MAY-2024, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2024, sequence version 1.
DT 28-JAN-2026, entry version 7.
DE RecName: Full=DNA primase large subunit {ECO:0000256|PIRNR:PIRNR009449};
GN ORFNames=N7460_006486 {ECO:0000313|EMBL:KAJ6045131.1};
OS Penicillium canescens.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX NCBI_TaxID=5083 {ECO:0000313|EMBL:KAJ6045131.1, ECO:0000313|Proteomes:UP001219568};
RN [1] {ECO:0000313|EMBL:KAJ6045131.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=IBT 15450 {ECO:0000313|EMBL:KAJ6045131.1};
RX PubMed=36726175;
RA Petersen C., Sorensen T., Nielsen M.R., Sondergaard T.E., Sorensen J.L.,
RA Fitzpatrick D.A., Frisvad J.C., Nielsen K.L.;
RT "Comparative genomic study of the Penicillium genus elucidates a diverse
RT pangenome and 15 lateral gene transfer events.";
RL IMA Fungus 14:0-0(2023).
RN [2] {ECO:0000313|EMBL:KAJ6045131.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=IBT 15450 {ECO:0000313|EMBL:KAJ6045131.1};
RA Petersen C.;
RL Submitted (JAN-2023) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA primase is the polymerase that synthesizes small RNA
CC primers for the Okazaki fragments made during discontinuous DNA
CC replication. {ECO:0000256|PIRNR:PIRNR009449}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|PIRNR:PIRNR009449};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000256|PIRNR:PIRNR009449};
CC -!- SIMILARITY: Belongs to the eukaryotic-type primase large subunit
CC family. {ECO:0000256|ARBA:ARBA00010564, ECO:0000256|PIRNR:PIRNR009449}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAJ6045131.1}.
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DR EMBL; JAQJZL010000004; KAJ6045131.1; -; Genomic_DNA.
DR AlphaFoldDB; A0AAD6NAQ4; -.
DR Proteomes; UP001219568; Unassembled WGS sequence.
DR GO; GO:0005658; C:alpha DNA polymerase:primase complex; IEA:TreeGrafter.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006270; P:DNA replication initiation; IEA:TreeGrafter.
DR GO; GO:0006269; P:DNA replication, synthesis of primer; IEA:UniProtKB-KW.
DR CDD; cd07322; PriL_PriS_Eukaryotic; 1.
DR FunFam; 1.20.930.80:FF:000003; DNA primase large subunit; 1.
DR Gene3D; 1.20.930.80; -; 1.
DR InterPro; IPR058560; DNA_primase_C.
DR InterPro; IPR016558; DNA_primase_lsu_euk.
DR InterPro; IPR007238; DNA_primase_lsu_euk/arc.
DR PANTHER; PTHR10537; DNA PRIMASE LARGE SUBUNIT; 1.
DR PANTHER; PTHR10537:SF3; DNA PRIMASE LARGE SUBUNIT; 1.
DR Pfam; PF04104; DNA_primase_lrg; 1.
DR Pfam; PF26466; DNA_primase_lrg_N; 1.
DR PIRSF; PIRSF009449; DNA_primase_large_subunit; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|PIRNR:PIRNR009449};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|PIRNR:PIRNR009449};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PIRNR:PIRNR009449};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRNR:PIRNR009449};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRNR:PIRNR009449};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRNR:PIRNR009449};
KW Primosome {ECO:0000256|ARBA:ARBA00022515, ECO:0000256|PIRNR:PIRNR009449};
KW Reference proteome {ECO:0000313|Proteomes:UP001219568}.
FT DOMAIN 298..485
FT /note="DNA primase large subunit C-terminal"
FT /evidence="ECO:0000259|Pfam:PF04104"
FT BINDING 308
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
FT BINDING 391
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
FT BINDING 408
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
FT BINDING 449
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
SQ SEQUENCE 501 AA; 58176 MW; 08DB77ED288C8C48 CRC64;
MIRQDAHRID PKRRAVIDHK KRQFAVPIYK QQEYPHRLNF YETPPTAEIT LEQFEQWAID
RLKILAEIEA CSYRNKSPAE TATYITPLLQ KFLPLSSNTS SPNGANDPRL KNERQKDHYS
HFILRLAFSA TEDLRRRFAR AETMLFRFRF QLDDSREKRA FIDSLSLDWE PVSDFERTEV
AESLASSTPG LHRVDEETWY KVDWEKVPEL IERRSVYLKK GKAYVPGREQ LSMIMAEFTA
RLERALELTS RALPRLDEDD RLTPILNHLS KNFGSAESVY SEGEGYVDGA PITASSIDQL
AQQHFPLCMR SLHVNLRKNN HLKHFGRLQY TLFLKGIGLS LEECILFWRQ SFKGKTDDEF
NSQYKYNIRH AYGEVGGDVN RRGKGYTPYS CQKILGDSNP GIGQTHGCPY RHFSVDNLVG
FLQATGVNDK ETLRGVREDV GKQRYHIACN RVFESTHKNE IKKVKEDGTW SQTDLDTIVH
PNTYFKRSFL LKQMARAPKE N
//
