ID A0AAD7JEJ9_9AGAR Unreviewed; 888 AA.
AC A0AAD7JEJ9;
DT 29-MAY-2024, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2024, sequence version 1.
DT 28-JAN-2026, entry version 9.
DE RecName: Full=Vacuolar transporter chaperone complex subunit 4 {ECO:0000256|ARBA:ARBA00067464};
DE EC=2.7.4.1 {ECO:0000256|ARBA:ARBA00012960};
DE AltName: Full=Polyphosphate kinase {ECO:0000256|ARBA:ARBA00080494};
DE AltName: Full=SPX-dependent polyphosphate polymerase VTC subunit 4 {ECO:0000256|ARBA:ARBA00075894};
DE AltName: Full=Vacuolar membrane polyphosphate polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00081313};
DE Flags: Fragment;
GN ORFNames=DFH07DRAFT_1020258 {ECO:0000313|EMBL:KAJ7762011.1};
OS Mycena maculata.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Marasmiineae; Mycenaceae; Mycena.
OX NCBI_TaxID=230809 {ECO:0000313|EMBL:KAJ7762011.1, ECO:0000313|Proteomes:UP001215280};
RN [1] {ECO:0000313|EMBL:KAJ7762011.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CBHHK188m {ECO:0000313|EMBL:KAJ7762011.1};
RG Lawrence Berkeley National Laboratory;
RA Harder C.B., Miyauchi S., Viragh M., Kuo A., Thoen E., Andreopoulos B.,
RA Lu D., Skrede I., Drula E., Henrissat B., Morin E., Kohler A., Barry K.,
RA LaButti K., Morin E., Salamov A., Lipzen A., Mereny Z., Hegedus B.,
RA Baldrian P., Stursova M., Weitz H., Taylor A., Grigoriev I.V., Nagy L.G.,
RA Martin F., Kauserud H.;
RT "Massive genome expansion in bonnet fungi (Mycena s.s.) driven by repeated
RT elements and novel gene families across ecological guilds.";
RL Submitted (MAR-2023) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[phosphate](n) + ATP = [phosphate](n+1) + ADP;
CC Xref=Rhea:RHEA:19573, Rhea:RHEA-COMP:9859, Rhea:RHEA-COMP:14280,
CC ChEBI:CHEBI:16838, ChEBI:CHEBI:30616, ChEBI:CHEBI:456216; EC=2.7.4.1;
CC Evidence={ECO:0000256|ARBA:ARBA00050204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19574;
CC Evidence={ECO:0000256|ARBA:ARBA00050204};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000256|ARBA:ARBA00004128};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004128}.
CC -!- SIMILARITY: Belongs to the VTC4 family.
CC {ECO:0000256|ARBA:ARBA00061390}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAJ7762011.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JARJLG010000044; KAJ7762011.1; -; Genomic_DNA.
DR AlphaFoldDB; A0AAD7JEJ9; -.
DR Proteomes; UP001215280; Unassembled WGS sequence.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IEA:TreeGrafter.
DR GO; GO:0033254; C:vacuolar transporter chaperone complex; IEA:TreeGrafter.
DR GO; GO:0008976; F:polyphosphate kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006799; P:polyphosphate biosynthetic process; IEA:UniProtKB-ARBA.
DR CDD; cd07751; PolyPPase_VTC4_like; 1.
DR CDD; cd14480; SPX_VTC2_like; 1.
DR FunFam; 3.20.100.30:FF:000001; Vacuolar transporter chaperone 4; 1.
DR Gene3D; 3.20.100.30; VTC, catalytic tunnel domain; 1.
DR InterPro; IPR003807; DUF202.
DR InterPro; IPR004331; SPX_dom.
DR InterPro; IPR051572; VTC_Complex_Subunit.
DR InterPro; IPR018966; VTC_domain.
DR InterPro; IPR042267; VTC_sf.
DR PANTHER; PTHR46140; VACUOLAR TRANSPORTER CHAPERONE 1-RELATED; 1.
DR PANTHER; PTHR46140:SF1; VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED; 1.
DR Pfam; PF02656; DUF202; 1.
DR Pfam; PF03105; SPX; 1.
DR Pfam; PF09359; VTC; 1.
DR PROSITE; PS51382; SPX; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP001215280};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Vacuole {ECO:0000256|ARBA:ARBA00022554}.
FT TRANSMEM 793..813
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 825..845
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 865..882
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1..204
FT /note="SPX"
FT /evidence="ECO:0000259|PROSITE:PS51382"
FT REGION 95..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 560..620
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 638..672
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 718..767
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 127..140
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 560..581
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 606..616
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 641..651
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 663..672
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 718..728
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 729..751
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 757..767
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KAJ7762011.1"
SQ SEQUENCE 888 AA; 100432 MW; 716B338AF24CF97D CRC64;
MKFGRKISTD LYNEWRPFYL DYNQLKRELK NRTTGRGWSV ADEKEFTVLL EKELDKIHDF
QKAKVRSTGS VRTKELSQRI RDAEKDVKRL VAEDAEYGPA SPRHRHRPQS SADPETQLRD
DSYLDGGSDD DSDTDSDEDI SSMGAESFDA LEARFHGLEE EVANLVADVH DLALYTKLNI
TGFMKILKKH DKQTNLPLKP TFVQDYLEKR PFYKYNWDAL IVKLSKLYDL VRTRGHPVQG
DSSAGGSQSA FVRQTTKYWV HPDNLLPLKL AILRNLPVLV FNPDKEFDAK DAAITSIYFD
NEDLELYLGR LEKTEGAEAI RLRWYGDTDV KTVFVERKTH REDWTGEKSV KARFPIKEHL
VNAFLRGEYT VDAEFQQLVK KGKKTQQEVD SMIQLANEVQ YAVLTRGLKP VMRSFYNRTA
FQLPGDARVR ISLDTELTLV REDNWDGRTR AGDNWRRTDI GIDFPFEQLP ADDKELFKYG
VLEVKLQTQF GQEPPKWVMD LVQSHLVEAV PKFSKFIHGC ATLLPNRVDL VPFWLPQMDT
DILKPDTGYL TIIERPANAV STAGSSTKAS SEHPTSPTTG MHSPADDEDA AYHEPVSEGE
EDDDRDLAPA KDEDKWTGLS GQEVAEAIAY REHMLKARSV TDGSPSSSKR NGVQAGPRHQ
AEDSDADDED EAAVAAGMAA GKGKGIPIMK PRTLSIDPLA PSSAFDETLR DRLKREKEVR
RGVKHHAEVE EEEEEETRPA TDPDEDEDVD DPLTSRPGDE RHLSREFRAP AGKRISVPVR
IEPKVYFAAE RTFLKWLNNA VFIGTIATAL LNFTAPEDSR GLMSAALFTL AALLAIAYSA
TIFVYRAHQL RAHRADGLYY DKYGPTLLCF VLFLALATNI GLRVSQML
//
