ID A0AAD7KSY7_QUISA Unreviewed; 393 AA.
AC A0AAD7KSY7;
DT 29-MAY-2024, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2024, sequence version 1.
DT 28-JAN-2026, entry version 9.
DE RecName: Full=U-box domain-containing protein {ECO:0000256|RuleBase:RU369093, ECO:0000259|PROSITE:PS51698};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU369093};
DE AltName: Full=RING-type E3 ubiquitin transferase PUB {ECO:0000256|RuleBase:RU369093};
GN ORFNames=O6P43_030325 {ECO:0000313|EMBL:KAJ7945232.1};
OS Quillaja saponaria (Soap bark tree).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Quillajaceae; Quillaja.
OX NCBI_TaxID=32244 {ECO:0000313|EMBL:KAJ7945232.1, ECO:0000313|Proteomes:UP001163823};
RN [1] {ECO:0000313|EMBL:KAJ7945232.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S10 {ECO:0000313|EMBL:KAJ7945232.1};
RX PubMed=36952412;
RA Reed J., Orme A., El-Demerdash A., Owen C., Martin L.B.B., Misra R.C.,
RA Kikuchi S., Rejzek M., Martin A.C., Harkess A., Leebens-Mack J.,
RA Louveau T., Stephenson M.J., Osbourn A.;
RT "Elucidation of the pathway for biosynthesis of saponin adjuvants from the
RT soapbark tree.";
RL Science 379:1252-1264(2023).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase.
CC {ECO:0000256|RuleBase:RU369093}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|RuleBase:RU369093};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU369093}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAJ7945232.1}.
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DR EMBL; JARAOO010000013; KAJ7945232.1; -; Genomic_DNA.
DR AlphaFoldDB; A0AAD7KSY7; -.
DR KEGG; qsa:O6P43_030325; -.
DR Proteomes; UP001163823; Chromosome 13.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR FunFam; 3.30.40.10:FF:000442; RING-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR058678; ARM_PUB.
DR InterPro; IPR045185; PUB22/23/24-like.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22849:SF103; U-BOX DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR22849; WDSAM1 PROTEIN; 1.
DR Pfam; PF25598; ARM_PUB; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP001163823};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU369093};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|RuleBase:RU369093}.
FT DOMAIN 2..76
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
SQ SEQUENCE 393 AA; 43810 MW; C647A9898F472F5B CRC64;
MTIPHLFRCP ISLDLFEDPV TLCTGQTYDR SCIEKWLAAG NLTCPVTMQK LQDPSIVPNH
TLRHLIDKWR QLGTEFDPGY SADIAFLSAL KHNLESDSDT ITIENKLQTL EKIRALSDEY
CSSRKSCFLQ LGFLSLLLEL VFGRVESQLP KDHMGFIELA LSCTIKLLPL GNLQALDMLK
EDSKLLSLQD LFEQGTIVIK ICLCHLIEAL ESPDTKEVCF IIGNTQRLLH QIVLLLYQNF
EASMAGVKAL TALCSLQCNK ENLVRSGAVD GIITYISSCE RQSLALMAMA TIENLLGLES
GKEALVNNPN GVEVLVKMVF RVSNQECSDT AVGSLLIVCY YSCRAREEAI GAGVLTQLLL
LLQSQCSNRT KTKARMLLKL LRSKWVEDHP NQV
//
