ID A0AAE1H8X5_9NEOP Unreviewed; 464 AA.
AC A0AAE1H8X5;
DT 29-MAY-2024, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2024, sequence version 1.
DT 28-JAN-2026, entry version 6.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN ORFNames=KUF71_025683 {ECO:0000313|EMBL:KAK3916568.1};
OS Frankliniella fusca.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Paraneoptera; Thysanoptera; Terebrantia; Thripoidea; Thripidae;
OC Frankliniella.
OX NCBI_TaxID=407009 {ECO:0000313|EMBL:KAK3916568.1, ECO:0000313|Proteomes:UP001219518};
RN [1] {ECO:0000313|EMBL:KAK3916568.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=PL_HMW_Pooled {ECO:0000313|EMBL:KAK3916568.1};
RC TISSUE=Head {ECO:0000313|EMBL:KAK3916568.1};
RA Catto M.A., Jacobson A., Kennedy G., Labadie P., Hunt B.G., Srinivasan R.;
RL Submitted (JUL-2021) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KAK3916568.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=PL_HMW_Pooled {ECO:0000313|EMBL:KAK3916568.1};
RX PubMed=37344773;
RA Catto M.A., Labadie P.E., Jacobson A.L., Kennedy G.G., Srinivasan R.,
RA Hunt B.G.;
RT "Pest status, molecular evolution, and epigenetic factors derived from the
RT genome assembly of Frankliniella fusca, a thysanopteran phytovirus
RT vector.";
RL BMC Genomics 24:0-0(2023).
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAK3916568.1}.
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DR EMBL; JAHWGI010000542; KAK3916568.1; -; Genomic_DNA.
DR AlphaFoldDB; A0AAE1H8X5; -.
DR Proteomes; UP001219518; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP001219518};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
SQ SEQUENCE 464 AA; 51380 MW; 2BC1B62427957EAE CRC64;
MCSRNEEEDG SLNVMEEKKL TWTDICQKCS GEKPVIQILQ IKHAYCRNCF LSHVNHKFRS
TLGKSKMMKP ADVVLAAFSG SGKSNAMLHL LHAGLSEQSH KRFLFKVQVL YIDEGAILGA
SALDRTASLH AVAKLADQYG FHSYYTTLSS IYDETGPKCI PLAQDSVLAI NKDSEQELTK
LFLNYMSTNT LTLLNRRKMF LQSAKNLNCS KVFVANTSTD LAIDILTDIS LGRGSHLHHD
VSFCDSRDEN VKIMRPLLEL SSKEVTYYSV FHHLESVHIP SLCTKASSDS SIHNMSKQFV
MDLMENFPST VSTVFRTGEK LGSTTTSTSC ETEDNLCIIC QVKLDENLTA CSSVQATDFS
RLISSLGPSG FDNKTLSLLP GNKADTALCG SSASCCVQSK NGYSCPTKTQ SLSLSEIENW
CCYGCRLILQ DMENVSSLPE SMLKAVQTRK SLEEMRSSIK DFLL
//
