ID A0AAF0CW30_9ENTE Unreviewed; 625 AA.
AC A0AAF0CW30;
DT 29-MAY-2024, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2024, sequence version 1.
DT 28-JAN-2026, entry version 9.
DE RecName: Full=Selenocysteine-specific elongation factor {ECO:0000256|ARBA:ARBA00015953};
DE AltName: Full=SelB translation factor {ECO:0000256|ARBA:ARBA00031615};
GN Name=selB {ECO:0000313|EMBL:WEG74073.1};
GN ORFNames=OL234_04015 {ECO:0000313|EMBL:WEG74073.1};
OS Vagococcus intermedius.
OC Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Vagococcus.
OX NCBI_TaxID=2991418 {ECO:0000313|EMBL:WEG74073.1, ECO:0000313|Proteomes:UP001179647};
RN [1] {ECO:0000313|EMBL:WEG74073.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=STAA11 {ECO:0000313|EMBL:WEG74073.1};
RA Johansson P., Bjorkroth J.;
RT "Vagococcus sp. isolated from poultry meat.";
RL Submitted (OCT-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Translation factor necessary for the incorporation of
CC selenocysteine into proteins. It probably replaces EF-Tu for the
CC insertion of selenocysteine directed by the UGA codon. SelB binds GTP
CC and GDP. {ECO:0000256|ARBA:ARBA00025526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; CP110232; WEG74073.1; -; Genomic_DNA.
DR RefSeq; WP_275469872.1; NZ_CP110232.1.
DR AlphaFoldDB; A0AAF0CW30; -.
DR KEGG; vie:OL234_04015; -.
DR Proteomes; UP001179647; Chromosome.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0001514; P:selenocysteine incorporation; IEA:InterPro.
DR CDD; cd04171; SelB; 1.
DR CDD; cd03696; SelB_II; 1.
DR CDD; cd15491; selB_III; 1.
DR Gene3D; 1.10.10.2770; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR057335; Beta-barrel_SelB.
DR InterPro; IPR050055; EF-Tu_GTPase.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR015190; Elong_fac_SelB-wing-hlx_typ-2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015191; SelB_WHD4.
DR InterPro; IPR005225; Small_GTP-bd.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004535; Transl_elong_SelB.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR NCBIfam; TIGR00475; selB; 1.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43721:SF22; ELONGATION FACTOR TU, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43721; ELONGATION FACTOR TU-RELATED; 1.
DR Pfam; PF25461; Beta-barrel_SelB; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF09106; WHD_2nd_SelB; 1.
DR Pfam; PF09107; WHD_3rd_SelB; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 2.
DR PROSITE; PS51722; G_TR_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Elongation factor {ECO:0000313|EMBL:WEG74073.1};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP001179647}.
FT DOMAIN 1..174
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 625 AA; 70530 MW; 691A047F89F101AE CRC64;
MDYKVMGTAG HIDHGKTTLI KALTGIQTDT TKEEQKRGLS INLGFAYLTL NHDNKLGIID
VPGHEKFVKN MLAGVAGIDF VLLVVDINEG VMPQTREHIA ILSLLGIKDF IVAITKTETV
DSEFRDMLVD DIKQDLLSLI PQAAPFILTD AVTGLGIPLL TREIIKFMQV IRPEKSHSLP
RLNVDRSFSI NGFGTVVTGT LIDGRLSVGD EVIIYPSEKK CKIRSLQVHE EPCETVYPGQ
RTALNLTKVK VKDIGRGDIL TKKNNIEPTW MLDVKVRCLN NDKFALKLWD RVHVHIGTKE
VIARVVPLGV EAIHAEEEGF IQLRLEEKVA VKQGDRLVLR SYSPVTTIAG GVVLEANPAK
HRRFNEQILA SLEIKESGKL ADIVRDVLKN HTKWVLTLKE IASELNKSGI LIQEALGELI
EQDEIKVIGP GFITTTHFEE GSQMILKLTS DYHTDNPFSP GIPKEEFREK IPMLKLKELE
EIMGKLIKDG KIVRQDEFIK IREFEVTCTE DLRNERVALE DLILKQGLLP QPYEELVTFS
KNAEDILNSM SNDQLIYLNQ TIVIHRQVYE QALNQITLFL KDNTTITIGD FRDLMGSSRK
YSILFLEYLD KQHITKRQGE GRILI
//
