ID A0AAF3E844_9BILA Unreviewed; 1024 AA.
AC A0AAF3E844;
DT 29-MAY-2024, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2024, sequence version 1.
DT 28-JAN-2026, entry version 8.
DE RecName: Full=Cyclin-dependent kinase 12 {ECO:0000256|ARBA:ARBA00040213};
DE EC=2.7.11.22 {ECO:0000256|ARBA:ARBA00012425};
DE EC=2.7.11.23 {ECO:0000256|ARBA:ARBA00012409};
DE AltName: Full=Cell division protein kinase 12 {ECO:0000256|ARBA:ARBA00041920};
OS Mesorhabditis belari.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Mesorhabditinae;
OC Mesorhabditis.
OX NCBI_TaxID=2138241 {ECO:0000313|Proteomes:UP000887575, ECO:0000313|WBParaSite:MBELARI_LOCUS10087.2};
RN [1] {ECO:0000313|WBParaSite:MBELARI_LOCUS10087.2}
RP IDENTIFICATION.
RG WormBaseParasite;
RL Submitted (FEB-2024) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP +
CC H(+); Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.22;
CC Evidence={ECO:0000256|ARBA:ARBA00048367};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] +
CC ADP + H(+); Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.22;
CC Evidence={ECO:0000256|ARBA:ARBA00047811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed
CC RNA polymerase] + ADP + H(+); Xref=Rhea:RHEA:10216, Rhea:RHEA-
CC COMP:11321, Rhea:RHEA-COMP:11322, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43176, ChEBI:CHEBI:68546,
CC ChEBI:CHEBI:456216; EC=2.7.11.23;
CC Evidence={ECO:0000256|ARBA:ARBA00049280};
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000256|ARBA:ARBA00004138}. Cytoplasm, cytoskeleton
CC {ECO:0000256|ARBA:ARBA00004245}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the BBS5 family.
CC {ECO:0000256|ARBA:ARBA00005822}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr
CC protein kinase family. CDC2/CDKX subfamily.
CC {ECO:0000256|ARBA:ARBA00006485}.
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DR AlphaFoldDB; A0AAF3E844; -.
DR WBParaSite; MBELARI_LOCUS10087.2; MBELARI_LOCUS10087.2; MBELARI_LOCUS10087.
DR Proteomes; UP000887575; Unassembled WGS sequence.
DR GO; GO:0034464; C:BBSome; IEA:InterPro.
DR GO; GO:0036064; C:ciliary basal body; IEA:TreeGrafter.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IEA:TreeGrafter.
DR GO; GO:0008353; F:RNA polymerase II CTD heptapeptide repeat kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0060271; P:cilium assembly; IEA:TreeGrafter.
DR FunFam; 1.10.510.10:FF:000415; CMGC/CDK/CRK7 protein kinase, variant; 1.
DR FunFam; 3.30.200.20:FF:000074; cyclin-dependent kinase 12 isoform X2; 1.
DR Gene3D; 3.30.200.20; Phosphorylase Kinase, domain 1; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR InterPro; IPR006606; BBL5.
DR InterPro; IPR014003; BBS5_PH.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR21351:SF0; BARDET-BIEDL SYNDROME 5 PROTEIN; 1.
DR PANTHER; PTHR21351; BARDET-BIEDL SYNDROME PROTEIN 5; 1.
DR Pfam; PF07289; BBL5; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00683; DM16; 2.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Cell projection {ECO:0000256|ARBA:ARBA00023273};
KW Cilium {ECO:0000256|ARBA:ARBA00023069};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Cytoskeleton {ECO:0000256|ARBA:ARBA00023212};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000887575};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 686..979
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT REGION 348..532
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 362..388
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..452
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 462..471
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 715
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 1024 AA; 115951 MW; 3A287DE49B1A273E CRC64;
MNKGDKINGE DVWQDREIRF DVDARMLRLI PGEVLVDKID NVEDTKGNNG DRGVMRVTNL
RLIWHASAMP RINLSIGYST VNGISAKKAN SKIRGEVESL YIMAKAPSTR FEFIFTALNP
GNVRLFTTVM SIHRAYETTK MYRELKMRGA VVDENEQLRI LPLEQQADRM GGVWNLSSDQ
GNLGVFIITN VRVVWYASMN PQYNVSIPYL QLFSCRVRDS KFGLALVLET TSTSGEYVLG
FRIDPEEKLQ QVCKAIQSLH KSFMMKPVFG VQVTRERPAT PRLGATEAMD TVEDDVEVLE
KMGRPDAFAA YFSDGIVSEE PRLPVFSEEL GLAVEQLKHE RLKLLNVGKM SRMTGKSGQN
GRSDRHKSES SSDTDRELYR AAKREHSPPR KRKDHSRNSP PPMSRSRRSR SPSPSNSRRG
DFREKRRSRD RSRSKSRGDR RRKDEKRRRG RSKSSEKDRK KSERKSKRKR YSSGESTDDL
VELREGSTLG SLIKKKQGDS EHKKKKKKGS KDSSSSSTSF DDETPIERSK IAGLQAPSNG
YYLPYGASLP NTGAGPSGIV FNSGDQAPSI STFHIPMPPG PAQTYSVMNI PVPPLMAQPI
HGAVHQSAQN LPPTIASAPI HAHFSLQQSQ QIVANIAPST LAEPVLTHLP PQAPHQPVRR
KRPTVLNAVQ RPVHFDPWGV GFVEQYDVKD QVGEGTYGQV YKAVNKFSGE QVALKKVRLE
NEKEGFPISA VREIKILRQL DHKNIVRLIN IVTDKTQVAD WRHTKNSFYL VFEYVDHDLM
GLLESHMVTF TDEQIAGLFK QLLEGLEYCH AKNFLHRDIK CSNILVNNKG ELKLADFGLA
RLFLHDVDRP YTNRVITLWY RPPELLLGEE KYGPSVDVWS VGCILGELYN KKPLFQGQTE
QSQLDLITKI CGSPTPQNWP DVANLPLYPT FIAKRVHPRN LKTALAFIPR ATLELLDKLL
ALDPKKRPTC SEALRHPFVA NIDPNKVKPL SLPIEQDCHE LWSKRLRKER CVQALQQELK
KKQE
//
