ID C4MAI0_ENTH1 Unreviewed; 252 AA.
AC C4MAI0; A0A175JZ88;
DT 07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 07-JUL-2009, sequence version 1.
DT 18-JUN-2025, entry version 83.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN ORFNames=EHI_191780 {ECO:0000313|EMBL:EAL43630.1};
OS Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM).
OC Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae;
OC Entamoeba.
OX NCBI_TaxID=294381 {ECO:0000313|EMBL:EAL43630.1, ECO:0000313|Proteomes:UP000001926};
RN [1] {ECO:0000313|EMBL:EAL43630.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HM-1:IMSS {ECO:0000313|EMBL:EAL43630.1};
RX PubMed=15729342; DOI=10.1038/nature03291;
RA Loftus B., Anderson I., Davies R., Alsmark U.C., Samuelson J., Amedeo P.,
RA Roncaglia P., Berriman M., Hirt R.P., Mann B.J., Nozaki T., Suh B., Pop M.,
RA Duchene M., Ackers J., Tannich E., Leippe M., Hofer M., Bruchhaus I.,
RA Willhoeft U., Bhattacharya A., Chillingworth T., Churcher C., Hance Z.,
RA Harris B., Harris D., Jagels K., Moule S., Mungall K., Ormond D.,
RA Squares R., Whitehead S., Quail M.A., Rabbinowitsch E., Norbertczak H.,
RA Price C., Wang Z., Guillen N., Gilchrist C., Stroup S.E., Bhattacharya S.,
RA Lohia A., Foster P.G., Sicheritz-Ponten T., Weber C., Singh U.,
RA Mukherjee C., El-Sayed N.M., Petri W.A.Jr., Clark C.G., Embley T.M.,
RA Barrell B., Fraser C.M., Hall N.;
RT "The genome of the protist parasite Entamoeba histolytica.";
RL Nature 433:865-868(2005).
RN [2] {ECO:0000313|EMBL:EAL43630.1}
RP GENOME REANNOTATION.
RC STRAIN=HM-1:IMSS {ECO:0000313|EMBL:EAL43630.1};
RA Lorenzi H., Amedeo P., Inman J., Schobel S., Caler E.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004167}; Single-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004167}.
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DR EMBL; DS571481; EAL43630.1; -; Genomic_DNA.
DR RefSeq; XP_649010.1; XM_643918.1.
DR AlphaFoldDB; C4MAI0; -.
DR FunCoup; C4MAI0; 1.
DR STRING; 5759.C4MAI0; -.
DR EnsemblProtists; GAT98812; GAT98812; CL6EHI_191780.
DR EnsemblProtists; rna_EHI_191780-1; rna_EHI_191780-1; EHI_191780.
DR GeneID; 3403302; -.
DR KEGG; ehi:EHI_191780; -.
DR VEuPathDB; AmoebaDB:EHI5A_014380; -.
DR VEuPathDB; AmoebaDB:EHI7A_170180; -.
DR VEuPathDB; AmoebaDB:EHI8A_187050; -.
DR VEuPathDB; AmoebaDB:EHI_191780; -.
DR VEuPathDB; AmoebaDB:KM1_020980; -.
DR HOGENOM; CLU_1104484_0_0_1; -.
DR InParanoid; C4MAI0; -.
DR OMA; CPDMACR; -.
DR OrthoDB; 28773at2759; -.
DR Proteomes; UP000001926; Partially assembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0000151; C:ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0031624; F:ubiquitin conjugating enzyme binding; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR FunFam; 1.20.120.1750:FF:000023; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.30.40.10:FF:000051; RBR-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR027370; Znf-RING_euk.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 1.
DR Pfam; PF22191; IBR_1; 1.
DR Pfam; PF13445; zf-RING_UBOX; 1.
DR SMART; SM00647; IBR; 2.
DR SMART; SM00184; RING; 2.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000001926};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 42..252
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 46..91
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
SQ SEQUENCE 252 AA; 29499 MW; A47E9BD9A56DCE60 CRC64;
MQTNYPSIDF KLLLKSDIVT RKKLIKEYID QRRMLLFYKR DDVVNCPICY NDVDQSFFYT
NPRCGHSFCL SCVSEYANEK IKQANGPILC PEKDCNEEIS YNDLINYGII SDPELLEQYN
STLTRIRIDN DPDTLYCIKC GTPMIGEPGI TMVRCVKCDY CFCCKCNEQW HADCTCEQYQ
RWKRENGMGD DAFQVYVKKN TKLCPQCHSP IEKNGGCNHI TCRCGFQFCW LCMQPYTKDH
WRVNRSGCTQ YS
//
