ID E3J0U0_PSEI1 Unreviewed; 367 AA.
AC E3J0U0;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 11-JAN-2011, sequence version 1.
DT 08-OCT-2025, entry version 67.
DE RecName: Full=Peptide chain release factor 2 {ECO:0000256|HAMAP-Rule:MF_00094, ECO:0000256|NCBIfam:TIGR00020};
DE Short=RF-2 {ECO:0000256|HAMAP-Rule:MF_00094};
GN Name=prfB {ECO:0000256|HAMAP-Rule:MF_00094};
GN OrderedLocusNames=FraEuI1c_6020 {ECO:0000313|EMBL:ADP84004.1};
OS Pseudofrankia inefficax (strain DSM 45817 / CECT 9037 / DDB 130130 / EuI1c)
OS (Frankia inefficax).
OC Bacteria; Bacillati; Actinomycetota; Actinomycetes; Frankiales;
OC Frankiaceae; Pseudofrankia.
OX NCBI_TaxID=298654 {ECO:0000313|EMBL:ADP84004.1, ECO:0000313|Proteomes:UP000002484};
RN [1] {ECO:0000313|EMBL:ADP84004.1, ECO:0000313|Proteomes:UP000002484}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 45817 / CECT 9037 / EuI1c
RC {ECO:0000313|Proteomes:UP000002484};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA Jeffries C., Kyrpides N., Ivanova N., Mikhailova N., Beauchemin N., Sen A.,
RA Sur S.A., Gtari M., Wall L., Tisa L., Woyke T.;
RT "Complete sequence of Frankia sp. EuI1c.";
RL Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC translation in response to the peptide chain termination codons UGA and
CC UAA. {ECO:0000256|ARBA:ARBA00002613, ECO:0000256|HAMAP-Rule:MF_00094}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00094}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF2. {ECO:0000256|HAMAP-Rule:MF_00094}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000256|ARBA:ARBA00010835, ECO:0000256|HAMAP-
CC Rule:MF_00094}.
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DR EMBL; CP002299; ADP84004.1; -; Genomic_DNA.
DR RefSeq; WP_013427122.1; NC_014666.1.
DR AlphaFoldDB; E3J0U0; -.
DR FunCoup; E3J0U0; 185.
DR STRING; 298654.FraEuI1c_6020; -.
DR KEGG; fri:FraEuI1c_6020; -.
DR eggNOG; COG1186; Bacteria.
DR HOGENOM; CLU_036856_6_0_11; -.
DR InParanoid; E3J0U0; -.
DR OrthoDB; 9806673at2; -.
DR Proteomes; UP000002484; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR FunFam; 3.30.160.20:FF:000004; Peptide chain release factor 1; 1.
DR Gene3D; 3.30.160.20; -; 1.
DR Gene3D; 3.30.70.1660; -; 1.
DR Gene3D; 1.20.58.410; Release factor; 1.
DR HAMAP; MF_00094; Rel_fac_2; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004374; PrfB.
DR NCBIfam; TIGR00020; prfB; 1.
DR PANTHER; PTHR43116:SF3; CLASS I PEPTIDE CHAIN RELEASE FACTOR; 1.
DR PANTHER; PTHR43116; PEPTIDE CHAIN RELEASE FACTOR 2; 1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; Release factor; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00094};
KW Methylation {ECO:0000256|ARBA:ARBA00022481, ECO:0000256|HAMAP-
KW Rule:MF_00094};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00094}; Reference proteome {ECO:0000313|Proteomes:UP000002484}.
FT DOMAIN 239..255
FT /note="Prokaryotic-type class I peptide chain release
FT factors"
FT /evidence="ECO:0000259|PROSITE:PS00745"
FT MOD_RES 246
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00094"
SQ SEQUENCE 367 AA; 40675 MW; 5B037131A4920C4A CRC64;
MAADIAEELK NLDSTLTGIE TVLDVEGLRR RAADLEQEAS DPDLWSDQDR AQVVTRKLSS
VRGDISRVEG LRDRLDNIMT AFELEDDDLI SEATGQLPTL ARDISTLEVR TLLSGEYDER
DAIVQLSAGA GGVDAADWTA MLLRMYLRWA ERHGYSTEVY DSSEAEEAGL KSATFLVKAP
YAYGTLRSEH GVHRLVRISP FDNQNRRQTS FAGVEITPVV ELSDHVDIDD KDLRVDIFRS
SGPGGQGVNT TDSAVRITHL PTGIVVTCQN ERSQLQNKAA AMVVLQAKLL ERRRAEEAAE
KQRLTGGPQD VSFGSQIRNY VLHPYQMVKD LRTEVETSNT GGVLDGEIDD FIDAEVRWRR
SAESGSH
//
