ID E9CKZ6_9GAMM Unreviewed; 572 AA.
AC E9CKZ6;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 28-JAN-2026, entry version 61.
DE RecName: Full=Selenocysteine-specific elongation factor {ECO:0000256|ARBA:ARBA00015953};
DE AltName: Full=SelB translation factor {ECO:0000256|ARBA:ARBA00031615};
GN Name=selB {ECO:0000313|EMBL:EFW12817.1};
GN ORFNames=SSYM_0862 {ECO:0000313|EMBL:EFW12817.1};
OS Serratia symbiotica str. Tucson.
OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC Enterobacterales; Yersiniaceae; Serratia; Serratia symbiotica.
OX NCBI_TaxID=914128 {ECO:0000313|EMBL:EFW12817.1, ECO:0000313|Proteomes:UP000013568};
RN [1] {ECO:0000313|Proteomes:UP000013568}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tucson {ECO:0000313|Proteomes:UP000013568};
RX PubMed=21266540;
RA Burke G.R., Moran N.A.;
RT "Massive genomic decay in Serratia symbiotica, a recently evolved symbiont
RT of aphids.";
RL Genome Biol. Evol. 3:195-208(2011).
CC -!- FUNCTION: Translation factor necessary for the incorporation of
CC selenocysteine into proteins. It probably replaces EF-Tu for the
CC insertion of selenocysteine directed by the UGA codon. SelB binds GTP
CC and GDP. {ECO:0000256|ARBA:ARBA00025526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; GL636105; EFW12817.1; -; Genomic_DNA.
DR AlphaFoldDB; E9CKZ6; -.
DR HOGENOM; CLU_023030_2_0_6; -.
DR Proteomes; UP000013568; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:InterPro.
DR GO; GO:0001514; P:selenocysteine incorporation; IEA:InterPro.
DR CDD; cd03696; SelB_II; 1.
DR FunFam; 1.10.10.10:FF:000350; Selenocysteine-specific translation elongation factor; 1.
DR FunFam; 2.40.30.10:FF:000080; Selenocysteine-specific translation elongation factor; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 2.
DR InterPro; IPR057335; Beta-barrel_SelB.
DR InterPro; IPR050055; EF-Tu_GTPase.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR015190; Elong_fac_SelB-wing-hlx_typ-2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015191; SelB_WHD4.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004535; Transl_elong_SelB.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR048649; WHD_1st_3rd_SelB.
DR InterPro; IPR048931; WHD_2nd_SelB_bact.
DR NCBIfam; TIGR00475; selB; 1.
DR PANTHER; PTHR43721:SF22; ELONGATION FACTOR TU, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43721; ELONGATION FACTOR TU-RELATED; 1.
DR Pfam; PF25461; Beta-barrel_SelB; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF21458; WHD_1st_3rd_SelB; 1.
DR Pfam; PF09106; WHD_2nd_SelB; 1.
DR Pfam; PF21214; WHD_2nd_SelB_bact; 1.
DR Pfam; PF09107; WHD_3rd_SelB; 1.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 2.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000013568}.
FT DOMAIN 14..125
FT /note="Tr-type G"
FT /evidence="ECO:0000259|Pfam:PF00009"
FT DOMAIN 148..215
FT /note="Translation elongation factor EFTu-like"
FT /evidence="ECO:0000259|Pfam:PF03144"
FT DOMAIN 225..303
FT /note="Selenocysteine-specific elongation factor beta-
FT barrel"
FT /evidence="ECO:0000259|Pfam:PF25461"
FT DOMAIN 309..356
FT /note="Selenocysteine-specific elongation factor first
FT winged helix"
FT /evidence="ECO:0000259|Pfam:PF21458"
FT DOMAIN 383..439
FT /note="Translation elongation factor SelB winged helix type
FT 2"
FT /evidence="ECO:0000259|Pfam:PF09106"
FT DOMAIN 446..504
FT /note="Selenocysteine-specific elongation factor second
FT winged helix"
FT /evidence="ECO:0000259|Pfam:PF21214"
FT DOMAIN 523..564
FT /note="Elongation factor SelB fourth winged-helix"
FT /evidence="ECO:0000259|Pfam:PF09107"
SQ SEQUENCE 572 AA; 62892 MW; 8341CA9FB5DFF86F CRC64;
MPTGHNLMAE CWGFIDVPGH EKFLANMLAG VGGIDHALLV VACDDGVMAQ TREHLAILRL
SGRLVLTVAL TKADRVDGAR IDVVCQQVQE ELARQGWHPT PLFVTAAVQG TGIKALCEHL
QTLLPGDHGL TRRFRLAVDR AFSIKGAGLV VTGTALGGRV TVGETLWLTG ADTPVRVRGL
HAQNQPVKQA QAGQRIALNI SGDVSKEQIA RGDWLLSHRP LAAAERILVA LDADLPIRHK
QPLHLHHAAS HITGRISLLN DNLAELILDR PLWLVENDRL VLRDIGARQT LGAAQVLSLS
VPKRGKRSPC FLVRLAAQAQ ASDDRQALAL LLSQGALQIA AFSWARQLTD SGLANLLAQH
QLLIAGGSAL AIDKVQQAES RLLQILADYH QQHADQLGLG RARLRRMALR ALDEALVFKM
IDNLLHIGAI SHTRGWLHLP EHALTFSTEE MAFWERIVPL FGGEAWWVRD LAAELGEDEV
RVRTLLRKAA LLDHVTAVVV DRYYLSSCIE QFAALIRELN VTQGSVNAVD FRDRLGIGRK
LAIQVLEFFD RSGFTRRKGN QHLLRDHGLF SE
//
