ID F6Q4L9_CIOIN Unreviewed; 395 AA.
AC F6Q4L9;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 2.
DT 10-JUN-2026, entry version 77.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
OS Ciona intestinalis (Transparent sea squirt) (Ascidia intestinalis).
OC Eukaryota; Metazoa; Chordata; Tunicata; Ascidiacea; Phlebobranchia;
OC Cionidae; Ciona.
OX NCBI_TaxID=7719 {ECO:0000313|Ensembl:ENSCINP00000013006.3, ECO:0000313|Proteomes:UP000008144};
RN [1] {ECO:0000313|Ensembl:ENSCINP00000013006.3, ECO:0000313|Proteomes:UP000008144}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=wild type {ECO:0000313|Ensembl:ENSCINP00000013006.3,
RC ECO:0000313|Proteomes:UP000008144};
RX PubMed=18854010; DOI=10.1186/gb-2008-9-10-r152;
RA Satou Y., Mineta K., Ogasawara M., Sasakura Y., Shoguchi E., Ueno K.,
RA Yamada L., Matsumoto J., Wasserscheid J., Dewar K., Wiley G.B.,
RA Macmil S.L., Roe B.A., Zeller R.W., Hastings K.E., Lemaire P.,
RA Lindquist E., Endo T., Hotta K., Inaba K.;
RT "Improved genome assembly and evidence-based global gene model set for the
RT chordate Ciona intestinalis: new insight into intron and operon
RT populations.";
RL Genome Biol. 9:R152-R152(2008).
RN [2] {ECO:0000313|Ensembl:ENSCINP00000013006.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (JAN-2026) to UniProtKB.
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; EAAA01002669; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; F6Q4L9; -.
DR FunCoup; F6Q4L9; 698.
DR STRING; 7719.ENSCINP00000013006; -.
DR Ensembl; ENSCINT00000013006.3; ENSCINP00000013006.3; ENSCING00000006318.3.
DR GeneTree; ENSGT00390000008797; -.
DR HOGENOM; CLU_024534_2_1_1; -.
DR InParanoid; F6Q4L9; -.
DR OMA; CHACRNI; -.
DR UniPathway; UPA00988; -.
DR Proteomes; UP000008144; Chromosome 8.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IBA:GO_Central.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IBA:GO_Central.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP000008144};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
SQ SEQUENCE 395 AA; 44380 MW; 796CDF5555E30C3E CRC64;
MCQVNGETFS PKLEPQIIHG KACVKCEKDS VIVVGTNGTF CKDCFEVYFS HKFRASMGRN
PLVRHHDKVA LAYSGGNNSS ALLNLIKKGL DPATVKKLKF TPGLIFVDER ILYPSDDYEK
VKEIEEIMRN TGFPYHIVKL EEVGAMCIYT LYIGIVYLIV NHICFNVNVM FKKEIPFLNH
VIFIIDQVFS TEDTDEKMTN AEKLEKLFNS ITTLTSKQET LYRLRCYLLS RKAKELGYDH
VFMGETSTRL AINILSNFSL GRGVHLPQDT GFSDGLRFEG VGVKLLRPMR EMSMKEIAVY
NALNKVPTVF LPNLATSQSV TSSVQNLTEN FILGLVAGFP STEMTVFRTS NKLHGKNDEK
KSRGCCRLCF GAIDTECGTA QATALRHTLF SEAIN
//
