ID G1MVG7_MELGA Unreviewed; 781 AA.
AC G1MVG7;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 3.
DT 28-JAN-2026, entry version 79.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 16 {ECO:0000256|ARBA:ARBA00019614, ECO:0000256|RuleBase:RU364149};
DE AltName: Full=Mediator complex subunit 16 {ECO:0000256|ARBA:ARBA00032015, ECO:0000256|RuleBase:RU364149};
GN Name=MED16 {ECO:0000256|RuleBase:RU364149,
GN ECO:0000313|Ensembl:ENSMGAP00000002639.3};
OS Meleagris gallopavo (Wild turkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Meleagridinae; Meleagris.
OX NCBI_TaxID=9103 {ECO:0000313|Ensembl:ENSMGAP00000002639.3, ECO:0000313|Proteomes:UP000001645};
RN [1] {ECO:0000313|Ensembl:ENSMGAP00000002639.3, ECO:0000313|Proteomes:UP000001645}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=20838655; DOI=10.1371/journal.pbio.1000475;
RA Dalloul R.A., Long J.A., Zimin A.V., Aslam L., Beal K., Blomberg L.A.,
RA Bouffard P., Burt D.W., Crasta O., Crooijmans R.P., Cooper K.,
RA Coulombe R.A., De S., Delany M.E., Dodgson J.B., Dong J.J., Evans C.,
RA Frederickson K.M., Flicek P., Florea L., Folkerts O., Groenen M.A.,
RA Harkins T.T., Herrero J., Hoffmann S., Megens H.J., Jiang A., de Jong P.,
RA Kaiser P., Kim H., Kim K.W., Kim S., Langenberger D., Lee M.K., Lee T.,
RA Mane S., Marcais G., Marz M., McElroy A.P., Modise T., Nefedov M.,
RA Notredame C., Paton I.R., Payne W.S., Pertea G., Prickett D., Puiu D.,
RA Qioa D., Raineri E., Ruffier M., Salzberg S.L., Schatz M.C., Scheuring C.,
RA Schmidt C.J., Schroeder S., Searle S.M., Smith E.J., Smith J.,
RA Sonstegard T.S., Stadler P.F., Tafer H., Tu Z.J., Van Tassell C.P.,
RA Vilella A.J., Williams K.P., Yorke J.A., Zhang L., Zhang H.B., Zhang X.,
RA Zhang Y., Reed K.M.;
RT "Multi-platform next-generation sequencing of the domestic turkey
RT (Meleagris gallopavo): genome assembly and analysis.";
RL PLoS Biol. 8:E1000475-E1000475(2010).
RN [2] {ECO:0000313|Ensembl:ENSMGAP00000002639.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (AUG-2025) to UniProtKB.
RN [3] {ECO:0000313|Ensembl:ENSMGAP00000002639.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2025) to UniProtKB.
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. {ECO:0000256|RuleBase:RU364149}.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000256|RuleBase:RU364149}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|RuleBase:RU364149}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 16 family.
CC {ECO:0000256|ARBA:ARBA00006543, ECO:0000256|RuleBase:RU364149}.
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DR AlphaFoldDB; G1MVG7; -.
DR Ensembl; ENSMGAT00000003320.3; ENSMGAP00000002639.3; ENSMGAG00000002986.3.
DR GeneID; 100541383; -.
DR KEGG; mgp:100541383; -.
DR CTD; 10025; -.
DR GeneTree; ENSGT00390000003821; -.
DR HOGENOM; CLU_018773_0_0_1; -.
DR InParanoid; G1MVG7; -.
DR OrthoDB; 10018574at2759; -.
DR Proteomes; UP000001645; Chromosome 30.
DR Bgee; ENSMGAG00000002986; Expressed in thymus and 17 other cell types or tissues.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IEA:TreeGrafter.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR048616; MED16_bridge.
DR InterPro; IPR048338; Mediator_Med16.
DR InterPro; IPR048339; Mediator_Med16_C.
DR InterPro; IPR021665; Mediator_Med16_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR13224:SF6; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 16; 1.
DR PANTHER; PTHR13224; THYROID HORMONE RECEPTOR-ASSOCIATED PROTEIN-RELATED; 1.
DR Pfam; PF20718; Med16_bridge; 1.
DR Pfam; PF20719; Med16_C; 1.
DR Pfam; PF11635; Med16_N; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Activator {ECO:0000256|ARBA:ARBA00023159, ECO:0000256|RuleBase:RU364149};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU364149};
KW Reference proteome {ECO:0000313|Proteomes:UP000001645};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU364149};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|RuleBase:RU364149};
KW WD repeat {ECO:0000256|ARBA:ARBA00022574, ECO:0000256|PROSITE-
KW ProRule:PRU00221}.
FT REPEAT 17..50
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT DOMAIN 63..361
FT /note="Mediator complex subunit Med16 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF11635"
FT DOMAIN 417..605
FT /note="Mediator of RNA polymerase II transcription subunit
FT 16 central helical bridge"
FT /evidence="ECO:0000259|Pfam:PF20718"
FT DOMAIN 679..774
FT /note="Mediator complex subunit 16 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20719"
SQ SEQUENCE 781 AA; 87053 MW; 4B3B4838E15BDC5B CRC64;
MVHIIDTEHP WDVHSVNSGH VEAITCLEWD QSGSRLLSAD ADGHIKCWSM TDHLANSWEN
TVGSMVEGDP VVALSWLHNG VKLALHVEKS GASNFGEKFS RVKFSPSLTL FGGKPMEGWI
AVTISGLVTV SLLKPNGQVL TSTESLCRLR CRVALADVAF TGGGNIVVAT SDGSSASPVQ
FYKVCVSVVN EKCKIDTEIL PSLFMRCTTD PARKDKYPAI THLKFLARDM SEQVLLCASN
QNNSIVECWS LRKEGLPVNN IFQQISPVVG DKQPMILKWR ILSATNDLDR VSAVALPKLP
ISLTNTDLKV ANDTKFFPGL GLALAFHDGS VHIVHRLSLQ MMAVFHGSSS QRPVDEQTIK
RQRTTGPLVH FKAMQLSWTS LALAGIDSHG KLSMLRISPS MGHVLDMNTS LRHLLFLLEY
CMVTGYDWWD ILLHVQPNMV QNLVEKLHEE YTRQSAALQQ VLATRIVAMK ASLCKLSSST
IARVCDYHAK LFLIAISCTL KSLLRPHFLN TPDKSPGDRL TEICSKITDI DIDKVMINLK
TEEFVLEMNT LQSLQQLIQW VGDFVLYLLA SLPNQGSPVR PGHSFLRDGP SLGMFRELMV
VIRIWGLLKP SCLPVYTATS DTQDSMSLLF RLLTKLWLCC REENHITEPD DTLIDECCLL
PSQLLIPNID WLPINDGIIS KLQNKQLVRL QFGKAPGLIG HTVSSQFDAF VRAPGQPKID
HLRRLHLGAY PTEECKSCTR CGCVTMLKSP NKVTAVKQWE QRWVKNCLCG GLWRRMPLSY
S
//
