ID G3NDC3_GASAC Unreviewed; 502 AA.
AC G3NDC3;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 27-NOV-2024, sequence version 2.
DT 10-JUN-2026, entry version 67.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Name=CTU2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Synonyms=NCS2 {ECO:0000256|HAMAP-Rule:MF_03054};
OS Gasterosteus aculeatus aculeatus (three-spined stickleback).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Cottioidei; Gasterosteales; Gasterosteidae;
OC Gasterosteus.
OX NCBI_TaxID=481459 {ECO:0000313|Ensembl:ENSGACP00000003323.2, ECO:0000313|Proteomes:UP000007635};
RN [1] {ECO:0000313|Ensembl:ENSGACP00000003323.2, ECO:0000313|Proteomes:UP000007635}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Lake Benthic {ECO:0000313|Ensembl:ENSGACP00000003323.2,
RC ECO:0000313|Proteomes:UP000007635};
RX PubMed=33598708;
RA Nath S., Shaw D.E., White M.A.;
RT "Improved contiguity of the threespine stickleback genome using long-read
RT sequencing.";
RL G3 (Bethesda) 11:0-0(2021).
RN [2] {ECO:0000313|Ensembl:ENSGACP00000003323.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (JAN-2026) to UniProtKB.
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
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DR RefSeq; XP_040034807.1; XM_040178873.1.
DR AlphaFoldDB; G3NDC3; -.
DR FunCoup; G3NDC3; 1782.
DR STRING; 69293.ENSGACP00000003323; -.
DR Ensembl; ENSGACT00000003334.2; ENSGACP00000003323.2; ENSGACG00000002530.2.
DR eggNOG; KOG2594; Eukaryota.
DR GeneTree; ENSGT00390000008797; -.
DR InParanoid; G3NDC3; -.
DR OMA; KQRKQMM; -.
DR OrthoDB; 25129at2759; -.
DR UniPathway; UPA00988; -.
DR Proteomes; UP000007635; Chromosome VI.
DR Bgee; ENSGACG00000002530; Expressed in mesonephros and 12 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP000007635};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
SQ SEQUENCE 502 AA; 54122 MW; DCA4D00228458437 CRC64;
MCQVEEDYHE PLQRADPLSV SKKCVKCKEG TAAVVIRAGD AFCRVCFKEY FIHKFRAMLG
KNRVIFPGEK VLLAVSGGAS SCSMLRQVQE GLSQNAHKKL RFSPGIVFID EGGAVGQAVE
DRRRAAAELQ AVFTATGLPF VTVPLEEVLD LPSSVLAAAS LVASGTGAYK AAVGDFVRSG
AAGCRTSLDP ERRAPADVQE SRTRSLQQLI GSAKTLTARE DLLSTLRQHL LVHTARSEGY
SKVMLGDNCT RLAIKLLTSI SLGRGAQLAQ DTGFSDSRYG DILLVRPMRD YSAKEIAYYN
RLFGVPSVFI PGLETKATDK SSIQRLTESF VTKLQADFPS TVSTIYRTSE KLQTACRSSS
TADLCDRCLL CMCALDTAAE NASAFKATLI SEQLSQAQAA GDTSVVTPAP KEHESAAAIG
KRCTSGAEDC GNAASGEGCC SSAKGAESSD LRSLLCYSCQ LTIRDMSSVE RLPHYILSEA
QRRQRRSQMR EEIGEFLLDA AD
//
