ID G8Y1Y1_PICSO Unreviewed; 971 AA.
AC G8Y1Y1;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 28-JAN-2026, entry version 56.
DE RecName: Full=Glucosidase II subunit alpha {ECO:0000256|ARBA:ARBA00042895};
GN Name=Piso0_005348 {ECO:0000313|EMBL:CCE86834.1};
GN ORFNames=GNLVRS01_PISO0N13169g {ECO:0000313|EMBL:CCE86834.1};
OS Pichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC
OS 10061 / NRRL Y-12695) (Hybrid yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Pichiomycetes;
OC Debaryomycetaceae; Millerozyma.
OX NCBI_TaxID=559304 {ECO:0000313|EMBL:CCE86834.1, ECO:0000313|Proteomes:UP000005222};
RN [1] {ECO:0000313|EMBL:CCE86834.1, ECO:0000313|Proteomes:UP000005222}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL
RC Y-12695 {ECO:0000313|Proteomes:UP000005222};
RX DOI=10.1534/g3.111.000745;
RA Leh Louis V., Despons L., Friedrich A., Martin T., Durrens P.,
RA Casaregola S., Neuveglise C., Fairhead C., Marck C., Cruz J.A.,
RA Straub M.L., Kugler V., Sacerdot C., Uzunov Z., Thierry A., Weiss S.,
RA Bleykasten C., De Montigny J., Jacques N., Jung P., Lemaire M., Mallet S.,
RA Morel G., Richard G.F., Sarkar A., Savel G., Schacherer J., Seret M.L.,
RA Talla E., Samson G., Jubin C., Poulain J., Vacherie B., Barbe V.,
RA Pelletier E., Sherman D.J., Westhof E., Weissenbach J., Baret P.V.,
RA Wincker P., Gaillardin C., Dujon B., Souciet J.L.;
RT "Pichia sorbitophila, an interspecies yeast hybrid reveals early steps of
RT genome resolution following polyploidization.";
RL G3 (Bethesda) 2:299-311(2012).
CC -!- PATHWAY: Glycan metabolism; N-glycan metabolism.
CC {ECO:0000256|ARBA:ARBA00004833}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000256|ARBA:ARBA00004240}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 31 family.
CC {ECO:0000256|ARBA:ARBA00007806, ECO:0000256|RuleBase:RU361185}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FO082046; CCE86834.1; -; Genomic_DNA.
DR AlphaFoldDB; G8Y1Y1; -.
DR FunCoup; G8Y1Y1; 1535.
DR STRING; 559304.G8Y1Y1; -.
DR eggNOG; KOG1066; Eukaryota.
DR HOGENOM; CLU_000631_7_0_1; -.
DR InParanoid; G8Y1Y1; -.
DR OMA; TVHQPLW; -.
DR OrthoDB; 1334205at2759; -.
DR Proteomes; UP000005222; Chromosome N.
DR GO; GO:0017177; C:glucosidase II complex; IEA:TreeGrafter.
DR GO; GO:0090599; F:alpha-glucosidase activity; IEA:TreeGrafter.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006491; P:N-glycan processing; IEA:TreeGrafter.
DR CDD; cd06603; GH31_GANC_GANAB_alpha; 1.
DR CDD; cd14752; GH31_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1760; glycosyl hydrolase (family 31); 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 2.
DR InterPro; IPR033403; DUF5110.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR017853; GH.
DR InterPro; IPR048395; Glyco_hydro_31_C.
DR InterPro; IPR025887; Glyco_hydro_31_N_dom.
DR InterPro; IPR000322; Glyco_hydro_31_TIM.
DR InterPro; IPR013780; Glyco_hydro_b.
DR PANTHER; PTHR22762; ALPHA-GLUCOSIDASE; 1.
DR PANTHER; PTHR22762:SF54; BCDNA.GH04962; 1.
DR Pfam; PF17137; DUF5110; 1.
DR Pfam; PF13802; Gal_mutarotas_2; 1.
DR Pfam; PF01055; Glyco_hydro_31_2nd; 1.
DR Pfam; PF21365; Glyco_hydro_31_3rd; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361185};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361185};
KW Reference proteome {ECO:0000313|Proteomes:UP000005222};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..971
FT /note="Glucosidase II subunit alpha"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003518938"
FT DOMAIN 90..318
FT /note="Glycoside hydrolase family 31 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF13802"
FT DOMAIN 363..693
FT /note="Glycoside hydrolase family 31 TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01055"
FT DOMAIN 702..796
FT /note="Glycosyl hydrolase family 31 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF21365"
FT DOMAIN 814..856
FT /note="DUF5110"
FT /evidence="ECO:0000259|Pfam:PF17137"
SQ SEQUENCE 971 AA; 110579 MW; 402D4C920767B8DD CRC64;
MKTSFSGIIL VLLIQLGFVL SYKEYLFKTC SSSGFCHRNR EFARNVQRAT NFVPQYAIDP
KSVEVDPELY VIKGQVLKHL AQLGKSVQLP FELTLLDKNN VRFQIFEDRS TAQPKNALVN
TARYNETTKW SFKTDQVTIE RVKDEKVIKD ENHVSFQFGK DGKYRAELSY SPVRLVIYAG
DEAQLVMNDN NFLNYEHFRT ENENSKHLSP YESDFDMFSE SFKETRHDKI PMGPESISSD
FTFKGFKHVY GIPEHADSFN LKDTTGSDLP YRLFNVDIFE YVPNSRMPMY GSIPFLLASK
PGVSVGLFWI NSADTFIDIN KKHEKDTSTH WMSENGVLDV VLTVGERPED INESYGSLTG
YVQLPPLFSL GYHQCRWNYN DEKDVLNINS LFDEHQIPYD VIWLDVDYAD SRKYFTWQPE
KFPDPEGMLK ELDITGRNLV LIIDTHLKTG YSLSDTIREK EITIKDSENK TFVGQCWPGE
AVWLDSMNPA SQEFWDEQHA LSDENTFMGR FSTNFYIWND MNEPSIFDGI ETTSLKSNLH
YGNWEHRSVH NVFGLTFHEA TYKALVKRLE STERQRPFIL TRSFYAGSQR TAAMWTGDNM
SKWEYLKASI PMVLTLGVSG MPFAGADVGG FFGDPSKELL TRWYQTGIWY PFFRAHAHID
SRRREPWVPG GSYTSIIRDA IRLRYALLPM WYTSFFESSQ TGVPVLKPTF YDNLNNIESY
DIEDQFYLGN SGILVKPVTD EGASSTEIYL PDEQIYYDFT GGCISNRSYQ LTEPGFITKD
VLLADIPMLL KGGSIVAAKN RYRRSSHLMA NDPYTLTVAL DKDGDASGRL YVDDGSSFNY
ERGEYLYLNF TANKTSIAST AENNSAFAES LGNIKVEKLQ LLGRRDITEA KAECDGETWN
LSFKTEGANT IVTNPKLPVN KNWTVHLQSD GSSQSAEEED KPKGALSSGL LVSVGAILAS
SAAALKYFSI L
//
