ID I0YXX8_COCSC Unreviewed; 958 AA.
AC I0YXX8;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 02-APR-2025, entry version 57.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN ORFNames=COCSUDRAFT_63603 {ECO:0000313|EMBL:EIE23247.1};
OS Coccomyxa subellipsoidea (strain C-169) (Green microalga).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Trebouxiophyceae incertae sedis; Elliptochloris clade; Coccomyxa;
OC Coccomyxa subellipsoidea.
OX NCBI_TaxID=574566 {ECO:0000313|EMBL:EIE23247.1, ECO:0000313|Proteomes:UP000007264};
RN [1] {ECO:0000313|EMBL:EIE23247.1, ECO:0000313|Proteomes:UP000007264}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C-169 {ECO:0000313|EMBL:EIE23247.1,
RC ECO:0000313|Proteomes:UP000007264};
RX PubMed=22630137; DOI=10.1186/gb-2012-13-5-r39;
RA Blanc G., Agarkova I., Grimwood J., Kuo A., Brueggeman A., Dunigan D.,
RA Gurnon J., Ladunga I., Lindquist E., Lucas S., Pangilinan J., Proschold T.,
RA Salamov A., Schmutz J., Weeks D., Yamada T., Claverie J.M., Grigoriev I.,
RA Van Etten J., Lomsadze A., Borodovsky M.;
RT "The genome of the polar eukaryotic microalga coccomyxa subellipsoidea
RT reveals traits of cold adaptation.";
RL Genome Biol. 13:R39-R39(2012).
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIE23247.1}.
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DR EMBL; AGSI01000008; EIE23247.1; -; Genomic_DNA.
DR RefSeq; XP_005647791.1; XM_005647734.1.
DR AlphaFoldDB; I0YXX8; -.
DR STRING; 574566.I0YXX8; -.
DR GeneID; 17041235; -.
DR KEGG; csl:COCSUDRAFT_63603; -.
DR eggNOG; KOG1815; Eukaryota.
DR OrthoDB; 514333at2759; -.
DR Proteomes; UP000007264; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF01485; IBR; 1.
DR Pfam; PF22191; IBR_1; 1.
DR SMART; SM00647; IBR; 2.
DR SMART; SM00184; RING; 3.
DR SUPFAM; SSF48403; Ankyrin repeat; 1.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000007264};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 375..605
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 379..425
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 255..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 349..370
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 706..760
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 718..735
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 958 AA; 102958 MW; 324D6A46FA79E936 CRC64;
MGAKNGTKKP LGPIPAKQIE LRRACQTGDV RKVASLLTAQ HSLWKVGIQR LVGSWVLRRF
GVHEDWLTPA RLDFVYDEEE ANMPIHYIAL GSPVSLVSRL LSSLCIRGPG AGHASDRAEL
LRFLLDKGYQ VDLTSVNARL ETPLHCALRS RSLEILEVLL THRPAAGAAQ VGAPVTEKDS
ESRRPLDVAL VSQQWPAVRL LIAAGALSKC PELEDARREL NQLLPAAEEG AKHGGVLFAL
PNALGKVFNF VLRQGQEPRP SPPPVGSSSA GPPLQEDPDE CSTESKASAI VLANNAEVAQ
HQQKSVEHIM YVLDVNHARA MALLEVNQWN EHRTVDAYFS DPEGALRAAG VSTESEASTS
EAAAPSTRAP AVPADGQGCI VCFEPVNPRK LSVALPCGHV TCDTCWKGIL KVRLSDGDVQ
RTGCPFVGCS CRLPFAVAQQ LLSKSQRERF EQLLAQSYAD TNPVIKWCPR AGCGRCLTVD
ARVGAADGVA AAAGNGRALD VRCSCGHAFC FSCLRAPHEP ATCAAVREWK SLVTEVKKEM
EERDEGWLAR NTKPCSGCGA PIQKNGGCNH IVCSRCRRQF CWICGGDWAS HNSATGGFYK
CNRFRAAVEA EAASQEGGAV GVRAFLGSVF GRIQDAAMLF RLNYYLRRYQ ASECDARQLH
VAAEWMSALL TAGLPLNHRS QGCEAEAGPG PTVASAAQES CPPARVGLGE LLDDTDGATS
ASEPCPSSPA ALAESNQSAS QEDSERPPDA VSKLAPQQWP EQQDAADYGY LKLAASEAIA
AREVLRNSYI AGFSLPWGDR RRHYEDIQGR LEAATEQCSV PLALLPDIQA IMQAGGCRAQ
PQDCSYLPAS APNRPLPIQV QDGEMLRRQF YFALAVHERA QQLRSLSKAV AAEKKLLLTS
ARKGLFEERA EASMDAAVAT GMLPLTGMQS EEMQGEGAGI GSALQTAGAA LAYFWHGS
//
