ID I3MLV2_ICTTR Unreviewed; 3171 AA.
AC I3MLV2;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 2.
DT 28-JAN-2026, entry version 62.
DE RecName: Full=FRAS1-related extracellular matrix protein 2 {ECO:0000256|ARBA:ARBA00074561};
DE AltName: Full=ECM3 homolog {ECO:0000256|ARBA:ARBA00075579};
GN Name=FREM2 {ECO:0000313|Ensembl:ENSSTOP00000012434.3};
OS Ictidomys tridecemlineatus (Thirteen-lined ground squirrel) (Spermophilus
OS tridecemlineatus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Sciuromorpha; Sciuridae;
OC Xerinae; Marmotini; Ictidomys.
OX NCBI_TaxID=43179 {ECO:0000313|Ensembl:ENSSTOP00000012434.3, ECO:0000313|Proteomes:UP000005215};
RN [1] {ECO:0000313|Proteomes:UP000005215}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Assembly & Analysis Group;
RG Computational R&D Group;
RG and Sequencing Platform;
RA Di Palma F., Alfoldi J., Johnson J., Berlin A., Gnerre S., Jaffe D.,
RA MacCallum I., Young S., Walker B.J., Lindblad-Toh K.;
RT "The Draft Genome of Spermophilus tridecemlineatus.";
RL Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSSTOP00000012434.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (AUG-2025) to UniProtKB.
RN [3] {ECO:0000313|Ensembl:ENSSTOP00000012434.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2025) to UniProtKB.
CC -!- FUNCTION: Extracellular matrix protein required for maintenance of the
CC integrity of the skin epithelium and for maintenance of renal
CC epithelia. Required for epidermal adhesion. Involved in the development
CC of eyelids and the anterior segment of the eyeballs.
CC {ECO:0000256|ARBA:ARBA00056394}.
CC -!- SUBUNIT: Interacts with FREM1. {ECO:0000256|ARBA:ARBA00065338}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004251};
CC Single-pass type I membrane protein {ECO:0000256|ARBA:ARBA00004251}.
CC -!- SIMILARITY: Belongs to the FRAS1 family.
CC {ECO:0000256|ARBA:ARBA00005529}.
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DR EMBL; AGTP01061657; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01061658; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01061659; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01061660; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01061661; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01061662; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01061663; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01061664; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01061665; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01061666; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_005330289.1; XM_005330232.4.
DR FunCoup; I3MLV2; 209.
DR STRING; 43179.ENSSTOP00000012434; -.
DR Ensembl; ENSSTOT00000013881.3; ENSSTOP00000012434.3; ENSSTOG00000013871.3.
DR GeneID; 101960884; -.
DR KEGG; iti:101960884; -.
DR CTD; 341640; -.
DR eggNOG; KOG3597; Eukaryota.
DR GeneTree; ENSGT00940000155313; -.
DR HOGENOM; CLU_001041_0_0_1; -.
DR InParanoid; I3MLV2; -.
DR OrthoDB; 430044at2759; -.
DR Proteomes; UP000005215; Unassembled WGS sequence.
DR GO; GO:0005604; C:basement membrane; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0007154; P:cell communication; IEA:InterPro.
DR GO; GO:0042733; P:embryonic digit morphogenesis; IEA:Ensembl.
DR GO; GO:0001654; P:eye development; IEA:Ensembl.
DR GO; GO:0007507; P:heart development; IEA:Ensembl.
DR GO; GO:0048839; P:inner ear development; IEA:Ensembl.
DR GO; GO:0001822; P:kidney development; IEA:Ensembl.
DR GO; GO:0002009; P:morphogenesis of an epithelium; IEA:Ensembl.
DR FunFam; 2.60.40.2030:FF:000008; FRAS1-related extracellular matrix protein 2; 1.
DR FunFam; 2.60.40.2030:FF:000011; Fras1-related extracellular matrix protein 2; 1.
DR FunFam; 2.60.40.2030:FF:000018; Fras1-related extracellular matrix protein 2; 1.
DR FunFam; 2.60.40.2030:FF:000040; Fras1-related extracellular matrix protein 2; 1.
DR FunFam; 2.60.40.2030:FF:000006; Fraser extracellular matrix complex subunit 1; 1.
DR Gene3D; 2.60.40.2030; -; 5.
DR InterPro; IPR038081; CalX-like_sf.
DR InterPro; IPR003644; Calx_beta.
DR InterPro; IPR039005; CSPG_rpt.
DR InterPro; IPR045658; FRAS1-rel_N.
DR InterPro; IPR051561; FRAS1_ECM.
DR PANTHER; PTHR45739:SF4; FRAS1-RELATED EXTRACELLULAR MATRIX PROTEIN 2; 1.
DR PANTHER; PTHR45739; MATRIX PROTEIN, PUTATIVE-RELATED; 1.
DR Pfam; PF16184; Cadherin_3; 12.
DR Pfam; PF03160; Calx-beta; 4.
DR Pfam; PF19309; Frem_N; 1.
DR SMART; SM00237; Calx_beta; 5.
DR SUPFAM; SSF141072; CalX-like; 5.
DR PROSITE; PS51854; CSPG; 12.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Cell adhesion {ECO:0000256|ARBA:ARBA00022889};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Developmental protein {ECO:0000256|ARBA:ARBA00022473};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000005215};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..45
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 46..3171
FT /note="FRAS1-related extracellular matrix protein 2"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5011665901"
FT TRANSMEM 3111..3136
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REPEAT 318..412
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT REPEAT 437..536
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT REPEAT 559..674
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT REPEAT 699..806
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT REPEAT 827..918
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT REPEAT 944..1036
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT REPEAT 1065..1167
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT REPEAT 1188..1281
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT REPEAT 1302..1398
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT REPEAT 1419..1511
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT REPEAT 1531..1620
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT REPEAT 1654..1751
FT /note="CSPG"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01201"
FT DOMAIN 1758..1857
FT /note="Calx-beta"
FT /evidence="ECO:0000259|SMART:SM00237"
FT DOMAIN 1870..1981
FT /note="Calx-beta"
FT /evidence="ECO:0000259|SMART:SM00237"
FT DOMAIN 1995..2102
FT /note="Calx-beta"
FT /evidence="ECO:0000259|SMART:SM00237"
FT DOMAIN 2117..2219
FT /note="Calx-beta"
FT /evidence="ECO:0000259|SMART:SM00237"
FT DOMAIN 2237..2341
FT /note="Calx-beta"
FT /evidence="ECO:0000259|SMART:SM00237"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3082..3104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3140..3171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3171 AA; 351137 MW; 2D163AAA6ED9AAE1 CRC64;
MHPGRTPEVA TRARRTANSA SSQPKLVPPP SLLLLLLSLV SRISAQLAVG DRALLSPGLA
GAAGVPSEEA IVMANRGLRV PFGREVWLDP LHDLVLQVQP GDRCAVTVLD NDALAQRPGR
LSPKRFPCDF GPGEVRYSHL GARSPSRDRV RLQLRYDAPG GAVVLPLVLE VEVVFTQLEI
VTRNLPLVVE ELLGTSNALD ARSLEFAYQS ETEECRVGIL TGLSALPRYG ELLHYPQVPG
EAREGSTSEP LLMDCKAFQE LGVRYRHTAP SRSPNRDWVP MVVELRSRGA PLGSPALKRE
HFQVLVRIRG GAENTAPKPS FVAMMMMEVD QFVLTALTPD MLAAEDAEST PDLLIFNLTA
PLQPGQGYLV STDDRSLPLS SFTQRDLHLL KIAYQPPSED SNQERLFELE LEVVDPEGAA
SDPFAFMVVV KPMNTLAPVV TRNTGLILYE GQSRPLTGPV GSGPQNLVIS DEDDLEAVRL
EVVAGLRHGH LVILGAPSGN SAPKTFTVAE LAAGHVVYQH DDKDGSLSDN LVLRMVDGGG
RHQVQFLFPI TLVPVDDQPP VLNANTGLTL AEGETVPIPP IALSATDIDS DDSLLLFVLE
SPSSTAGHLL LRQTHPPQEE QELISGLWRK QGAYYERTVT EWQQKDITEG RLFYRHSGPH
SPGPVMDQFT FRVQDNHDPP NQSGLQKFVI RIHPVDRLPP ELVSGCPLRM VVQESQLTPL
RKKWLRYTDL DTDDRELRYT VTQPPVDTDE NHSPVPLGTL VLTDYPSTVV THFTQAQINH
HKIAYRPPGQ ELGVAARVAQ FQFQVEDRAG NVAPGTFTLY LQPVDNQPPD ILNTGFTIQE
KGHHILSETE LHVSDVDTDM AHIIFTLTQA PKHGHMQVSG QTLHVGELFH LEDIKQGRIS
YTHNGDESLT DSCSLEVSDR HHVVPITLRV NVRPVDQEVP MLSLPTGTLE SYLDVLENGA
TEITTSVIKG TDEHTDDLML TFLLEDPPLY GEILVNGAPA EQFTQKDLLE GSVVYAHTSG
EIGLLPKGDS FNLSLSEKSQ EWRMGGNTIQ GVTVWVTILP VDSQAPEISV GEQFTVMEGD
KGVITSMHIS AEDIDSLNDD LLCTIVIQPT SGYVENISPA PGSEKSRAGI AISAFTLKDL
RQGHINYVQS VHKGVEPVED RFVFRCSDGI NFSERHFFPI VIIPTNDEKP EMFMREFIVM
EGMSLVIDTP ILNAADADIP PDDLTFTITR FPTHGHIMSQ QVNGTVLVES FTLDQIIESS
SIIYEHDDSE TQEDSFVIKL TDGEHSLEKV ALIMILPVDD ETPRMAINNG LEIEIGETKV
INNKILMATD LDSEDKSLAY IIRYGPGHGV LQRRKLTGAF ENITLGMNFT QDEVDRNLIQ
YVHFGQEGIR DLIKFDVTDG INALIDRYFF VSIGSVDIVF PDVISKGVSL KEGGKVTLTT
DLLSTSDLNS PDENLVFTIT RAPMRGHLEC TDQPGVSIAS FTQLQLAGNK IYYIHTADDE
VKMDSFEFQV TDGRNPVFRT FRISISDVDN KKPVVTIHNL VVSEGENKLI TPFELTVEDR
DTPDKLLKFT VTQVPVHGHL VFNNTKPATA FTKQDLNENL ISYKHDGTES SEDSFSFTVT
DGTHTDFYVF PDTVFETRRP QVMKIQILPV DNSVPQIAVN KGASVLRNLA TGHLGFMITS
KILKVEDRDS LHFSLKFTVT EAPRHGYLFN LDKGNHSITQ FTQADIDDMK ICYVLKEGAN
ATSDVFHFAV EDGGGNKLTN QHFRLNWAWI SFEKEYNLIN EDSKFLDVVL KRRGYLGETS
FISIGTRDGT AEKDKDFKGK AQKQVQFNPG QTRATWRVRI LSDGEHEQSE TFQVVLSEPV
LAALEFPAVT TVEIVDPGDE STVFIPQSEY SIEEDVGELF IPIRRTGDVS QEFMVICYTQ
QGTATGTVPT SVLSYSDYIS RPEDHTSVIR FDKDEREKMC RIVVIDDSLY EEEETFRVLL
SMPMGGRIGS EFPGAQVTIL PDRDDEPSFY FGNVQYSVDE SSGHVEVQVW RTGTDLSRPS
SVTVRSRKTD PPSADAGTDY VGISRNLDFA PGVNMQTVRV IILDDLGQPV LEGTEKFELV
LRMPVNAVLG EPSKATVFIN DSVSDLPKMQ FKERVYTGSE SDGHIAAMIH RSGDIQYRSS
VRCYTRQGSA QVMMDFEERP NTDVSIITFL PGEIEKPCVL ELMDDILYEE VEELRLVLGT
PQSSSPFGAA VGEQNETLIR IRDDADKTII KFGETKFSVT EPREPGESVV VEIPVIRQGD
TSKVSIVRVH TKDGSATSGE DYHPVSEEIE FKEGETQHIV EIEVIFDGVR EMREAFTVHL
KPDENMIAET QVTKAIVYIE EMNSMADVTF PSVPQIVSLL MYDDTAKAKE SAGPVSGYPI
ICVTACNPKY SDYDKTGSIC ASENINDTLT RYRWLISAPA GPDGVTSPMR EVDFDTFFTS
SKMITLDSIY FQPGSRVQCA ARAVNTNGNE GLELTSPIVT IAREEGLCQP RVPGVVGAEP
FSAKLRYTGP EDPDYSNLIK LTVTMPHIDG MLPVISTREL SNFELTLSPD GTRVGNHKCS
NLLDYTEVKT HHGFLTDATK NPEVIGESYP YQYSSPLRGN GTLRFYRNLN LEACLWEFVS
YYDMSELLAD CGGTIGTDGQ VLNLVQSYVT LRVPLYVSYV FHSPVGVGGW QHFDLKSELR
LTFVYDTAIL WSNGIGSPPG AELQGSLYPT SMRIREEGRL AVNFKTQAQF HGLFVLSHPA
SFTSSMIMSA DHPGLTFSLR LVRSEPTYNQ PIQQWSFVSD FAVRDYSGTY TVKLVPCTTP
SNQEYRLPVT CNPREPVTFD LDIRFQQVSD PVAAEFSLNT QMYLLSKKSL WLSDGSMGFG
QESDVAFAEG DTIYGRVMVD PVQNLGDSFY CSIEKVFLCT GADGYVPKYS PTNEEYGCLA
DSPSLLYRFK IVDKAQPETQ ATSFGNVLFN AKLAVDDPEA ILLVNQPGSD GFKVDSTPLF
QVALGREWYI HTIYTVRSKD NTNRGIGKRS VEYEYHSLVH QGKPQITTGS RKKREIRSAP
PLAWDIGAEN NRGTNIQHIA LDRANKRQTP PGRVPPEGIL PRELNSPSSE VSLVTIVGSI
AVGLLTICLT VIAAMMCKSK KSVRGKDTPK GSGSSEPMMP PQSHHSDSSE V
//
