ID ORML3_AILME Reviewed; 153 AA.
AC D2I2F3;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 09-FEB-2010, sequence version 1.
DT 02-SEP-2026, entry version 65.
DE RecName: Full=ORM1-like protein 3;
GN Name=ORMDL3; ORFNames=PANDA_019568;
OS Ailuropoda melanoleuca (Giant panda).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Ursidae; Ailuropoda.
OX NCBI_TaxID=9646;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=20010809; DOI=10.1038/nature08696;
RA Li R., Fan W., Tian G., Zhu H., He L., Cai J., Huang Q., Cai Q., Li B.,
RA Bai Y., Zhang Z., Zhang Y., Wang W., Li J., Wei F., Li H., Jian M., Li J.,
RA Zhang Z., Nielsen R., Li D., Gu W., Yang Z., Xuan Z., Ryder O.A.,
RA Leung F.C., Zhou Y., Cao J., Sun X., Fu Y., Fang X., Guo X., Wang B.,
RA Hou R., Shen F., Mu B., Ni P., Lin R., Qian W., Wang G., Yu C., Nie W.,
RA Wang J., Wu Z., Liang H., Min J., Wu Q., Cheng S., Ruan J., Wang M.,
RA Shi Z., Wen M., Liu B., Ren X., Zheng H., Dong D., Cook K., Shan G.,
RA Zhang H., Kosiol C., Xie X., Lu Z., Zheng H., Li Y., Steiner C.C.,
RA Lam T.T., Lin S., Zhang Q., Li G., Tian J., Gong T., Liu H., Zhang D.,
RA Fang L., Ye C., Zhang J., Hu W., Xu A., Ren Y., Zhang G., Bruford M.W.,
RA Li Q., Ma L., Guo Y., An N., Hu Y., Zheng Y., Shi Y., Li Z., Liu Q.,
RA Chen Y., Zhao J., Qu N., Zhao S., Tian F., Wang X., Wang H., Xu L., Liu X.,
RA Vinar T., Wang Y., Lam T.W., Yiu S.M., Liu S., Zhang H., Li D., Huang Y.,
RA Wang X., Yang G., Jiang Z., Wang J., Qin N., Li L., Li J., Bolund L.,
RA Kristiansen K., Wong G.K., Olson M., Zhang X., Li S., Yang H., Wang J.,
RA Wang J.;
RT "The sequence and de novo assembly of the giant panda genome.";
RL Nature 463:311-317(2010).
CC -!- FUNCTION: Plays an essential role in the homeostatic regulation of
CC sphingolipid de novo biosynthesis by modulating the activity of the
CC serine palmitoyltransferase (SPT) in response to ceramide levels. When
CC complexed to SPT, the binding of ceramides to its N-terminus stabilizes
CC a conformation that block SPT substrate entry, hence preventing SPT
CC catalytic activity. Through this mechanism, maintains ceramide levels
CC at sufficient concentrations for the production of complex
CC sphingolipids, but which prevents the accumulation of ceramides to
CC levels that trigger apoptosis. {ECO:0000250|UniProtKB:Q8N138}.
CC -!- SUBUNIT: Ceramide-sensitive subunit of the serine palmitoyltransferase
CC (SPT) complex, which is also composed of SPTLC1, SPTLC2/3 and SPTSSA/B.
CC {ECO:0000250|UniProtKB:Q8N138}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q8N138}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q8N138}.
CC -!- DOMAIN: Ceramides bind to ORMDL3 N-terminus and stabilize it in a
CC conformation that physically restricts the accessibility of the
CC substrates to their binding sites in the serine palmitoyltransferase
CC (SPT) complex, hence inhibiting SPT catalytic activity. In the absence
CC of ceramides, the N-terminus is flexible and permits substrate binding,
CC thus liberating SPT from inhibition. {ECO:0000250|UniProtKB:Q8N138}.
CC -!- PTM: When hydroxylated at Pro-137, ubiquitinated via 'Lys-48'-linkage,
CC leading to proteasomal degradation. In endothelial cells, ORMDL3
CC proteasomal degradation is controlled by the sphingosine 1-phosphate
CC receptor signaling pathway. {ECO:0000250|UniProtKB:Q8N138}.
CC -!- SIMILARITY: Belongs to the ORM family. {ECO:0000305}.
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DR EMBL; GL194160; EFB23385.1; -; Genomic_DNA.
DR RefSeq; XP_011235676.1; XM_011237374.3.
DR AlphaFoldDB; D2I2F3; -.
DR SMR; D2I2F3; -.
DR STRING; 9646.ENSAMEP00000010359; -.
DR GeneID; 100479284; -.
DR KEGG; aml:100479284; -.
DR CTD; 94103; -.
DR eggNOG; KOG3319; Eukaryota.
DR HOGENOM; CLU_072117_3_0_1; -.
DR InParanoid; D2I2F3; -.
DR OMA; WTAYILI; -.
DR OrthoDB; 1932233at2759; -.
DR Proteomes; UP000008912; Unassembled WGS sequence.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0017059; C:serine palmitoyltransferase complex; ISS:UniProtKB.
DR GO; GO:0006672; P:ceramide metabolic process; ISS:UniProtKB.
DR InterPro; IPR007203; ORMDL.
DR PANTHER; PTHR12665; ORMDL PROTEINS; 1.
DR Pfam; PF04061; ORMDL; 1.
DR PIRSF; PIRSF018147; ORMDL; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Hydroxylation; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Ubl conjugation.
FT CHAIN 1..153
FT /note="ORM1-like protein 3"
FT /id="PRO_0000394251"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8N138, ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q8N138, ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q8N138, ECO:0000255"
FT TOPO_DOM 64..94
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8N138, ECO:0000255"
FT TRANSMEM 95..117
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q8N138, ECO:0000255"
FT TOPO_DOM 118..121
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q8N138, ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q8N138, ECO:0000255"
FT TOPO_DOM 143..153
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8N138, ECO:0000255"
FT REGION 1..17
FT /note="Important for ceramide level-sensing"
FT /evidence="ECO:0000250|UniProtKB:Q8N138"
FT MOD_RES 137
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:Q8N138"
SQ SEQUENCE 153 AA; 17451 MW; B5D2C1AE6557D42A CRC64;
MNVGTAHSEV NPNTRVMNSR GIWLSYVLAI GLLHVVLLSI PFVSVPVVWT LTNLIHNTGM
YIFLHTVKGT PFETPDQGKA RLLTHWEQMD YGVQFTASRK FLTITPIVLY FLTSFYTKYD
QIHFVLNTVS LMSVLIPKLP QLHGVRIFGI NKY
//
