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Database: UniProt
Entry: P42919
LinkDB: P42919
Original site: P42919 
ID   RL2_BACSU               Reviewed;         277 AA.
AC   P42919;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 3.
DT   29-SEP-2021, entry version 145.
DE   RecName: Full=50S ribosomal protein L2 {ECO:0000255|HAMAP-Rule:MF_01320};
DE            Short=BL2;
GN   Name=rplB {ECO:0000255|HAMAP-Rule:MF_01320}; OrderedLocusNames=BSU01190;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SG38;
RX   PubMed=9371452; DOI=10.1128/jb.179.22.7046-7054.1997;
RA   Li X., Lindahl L., Sha Y., Zengel J.M.;
RT   "Analysis of the Bacillus subtilis S10 ribosomal protein gene cluster
RT   identifies two promoters that may be responsible for transcription of the
RT   entire 15-kilobase S10-spc-alpha cluster.";
RL   J. Bacteriol. 179:7046-7054(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA   Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA   Kawamura F., Yoshikawa H., Takahashi H.;
RT   "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT   subtilis chromosome.";
RL   Microbiology 142:3039-3046(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   SEQUENCE REVISION TO 9; 256 AND 262.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [5]
RP   PROTEIN SEQUENCE OF 2-9, AND SUBUNIT.
RC   STRAIN=PY22;
RX   PubMed=10781545; DOI=10.1128/jb.182.10.2771-2777.2000;
RA   Scott J.M., Ju J., Mitchell T., Haldenwang W.G.;
RT   "The Bacillus subtilis GTP binding protein obg and regulators of the
RT   sigma(B) stress response transcription factor cofractionate with
RT   ribosomes.";
RL   J. Bacteriol. 182:2771-2777(2000).
RN   [6] {ECO:0007744|PDB:6HA1, ECO:0007744|PDB:6HA8}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.10 ANGSTROMS) OF 1-277 WITH AND WITHOUT
RP   VIRGINIAMYCIN M.
RX   PubMed=30126986; DOI=10.1073/pnas.1808535115;
RA   Crowe-McAuliffe C., Graf M., Huter P., Takada H., Abdelshahid M.,
RA   Novacek J., Murina V., Atkinson G.C., Hauryliuk V., Wilson D.N.;
RT   "Structural basis for antibiotic resistance mediated by the Bacillus
RT   subtilis ABCF ATPase VmlR.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:8978-8983(2018).
CC   -!- FUNCTION: One of the primary rRNA binding proteins. Required for
CC       association of the 30S and 50S subunits to form the 70S ribosome, for
CC       tRNA binding and peptide bond formation. It has been suggested to have
CC       peptidyltransferase activity; this is somewhat controversial. Makes
CC       several contacts with the 16S rRNA in the 70S ribosome.
CC       {ECO:0000255|HAMAP-Rule:MF_01320}.
CC   -!- SUBUNIT: Part of the 50S ribosomal subunit (PubMed:10781545,
CC       PubMed:30126986). Forms a bridge to the 30S subunit in the 70S ribosome
CC       (By similarity). {ECO:0000255|HAMAP-Rule:MF_01320,
CC       ECO:0000269|PubMed:10781545, ECO:0000269|PubMed:30126986}.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uL2 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01320}.
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DR   EMBL; U43929; AAC45959.1; -; Genomic_DNA.
DR   EMBL; D50302; BAA08834.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11895.2; -; Genomic_DNA.
DR   PIR; F69694; F69694.
DR   RefSeq; NP_388000.2; NC_000964.3.
DR   RefSeq; WP_003225795.1; NZ_JNCM01000029.1.
DR   PDB; 3J3V; EM; 13.30 A; C=1-277.
DR   PDB; 3J3W; EM; 10.70 A; C=1-277.
DR   PDB; 3J9W; EM; 3.90 A; BD=1-277.
DR   PDB; 5NJT; EM; 3.80 A; W=2-276.
DR   PDB; 6HA1; EM; 3.10 A; C=1-277.
DR   PDB; 6HA8; EM; 3.50 A; C=1-277.
DR   PDB; 6HTQ; EM; 4.50 A; C=2-273.
DR   PDB; 6PPF; EM; 3.40 A; C=1-277.
DR   PDB; 6PPK; EM; 4.40 A; C=1-277.
DR   PDB; 6PVK; EM; 3.40 A; C=1-277.
DR   PDB; 6TNN; EM; 3.07 A; W=1-277.
DR   PDB; 6TPQ; EM; 3.07 A; W=1-277.
DR   PDB; 7AQC; EM; 2.99 A; C=1-277.
DR   PDB; 7AQD; EM; 3.10 A; C=1-277.
DR   PDB; 7AS8; EM; 2.90 A; E=1-277.
DR   PDB; 7AS9; EM; 3.50 A; E=1-277.
DR   PDBsum; 3J3V; -.
DR   PDBsum; 3J3W; -.
DR   PDBsum; 3J9W; -.
DR   PDBsum; 5NJT; -.
DR   PDBsum; 6HA1; -.
DR   PDBsum; 6HA8; -.
DR   PDBsum; 6HTQ; -.
DR   PDBsum; 6PPF; -.
DR   PDBsum; 6PPK; -.
DR   PDBsum; 6PVK; -.
DR   PDBsum; 6TNN; -.
DR   PDBsum; 6TPQ; -.
DR   PDBsum; 7AQC; -.
DR   PDBsum; 7AQD; -.
DR   PDBsum; 7AS8; -.
DR   PDBsum; 7AS9; -.
DR   SMR; P42919; -.
DR   IntAct; P42919; 1.
DR   MINT; P42919; -.
DR   STRING; 224308.BSU01190; -.
DR   jPOST; P42919; -.
DR   PaxDb; P42919; -.
DR   PRIDE; P42919; -.
DR   EnsemblBacteria; CAB11895; CAB11895; BSU_01190.
DR   GeneID; 50135140; -.
DR   GeneID; 64301957; -.
DR   GeneID; 936817; -.
DR   KEGG; bsu:BSU01190; -.
DR   PATRIC; fig|224308.179.peg.122; -.
DR   eggNOG; COG0090; Bacteria.
DR   InParanoid; P42919; -.
DR   OMA; SCIELRP; -.
DR   PhylomeDB; P42919; -.
DR   BioCyc; BSUB:BSU01190-MONOMER; -.
DR   PRO; PR:P42919; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR   GO; GO:0016740; F:transferase activity; IEA:InterPro.
DR   GO; GO:0002181; P:cytoplasmic translation; IBA:GO_Central.
DR   Gene3D; 2.30.30.30; -; 1.
DR   Gene3D; 4.10.950.10; -; 1.
DR   HAMAP; MF_01320_B; Ribosomal_L2_B; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR022666; Rbsml_prot_L2_RNA-bd_dom.
DR   InterPro; IPR014722; Rib_L2_dom2.
DR   InterPro; IPR002171; Ribosomal_L2.
DR   InterPro; IPR005880; Ribosomal_L2_bac/org-type.
DR   InterPro; IPR022669; Ribosomal_L2_C.
DR   InterPro; IPR022671; Ribosomal_L2_CS.
DR   InterPro; IPR014726; Ribosomal_L2_dom3.
DR   InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR   PANTHER; PTHR13691; PTHR13691; 1.
DR   Pfam; PF00181; Ribosomal_L2; 1.
DR   Pfam; PF03947; Ribosomal_L2_C; 1.
DR   PIRSF; PIRSF002158; Ribosomal_L2; 1.
DR   SMART; SM01383; Ribosomal_L2; 1.
DR   SMART; SM01382; Ribosomal_L2_C; 1.
DR   SUPFAM; SSF50104; SSF50104; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR01171; rplB_bact; 1.
DR   PROSITE; PS00467; RIBOSOMAL_L2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Reference proteome;
KW   Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:10781545"
FT   CHAIN           2..277
FT                   /note="50S ribosomal protein L2"
FT                   /id="PRO_0000129529"
FT   REGION          219..277
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        256..277
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        9
FT                   /note="T -> S (in Ref. 1; AAC45959 and 2; BAA08834)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        116
FT                   /note="I -> V (in Ref. 1; AAC45959)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        256
FT                   /note="G -> E (in Ref. 2; BAA08834)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        262
FT                   /note="K -> Q (in Ref. 2; BAA08834)"
FT                   /evidence="ECO:0000305"
FT   STRAND          3..5
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   TURN            11..15
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          17..19
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   TURN            31..33
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          34..36
FT                   /evidence="ECO:0007829|PDB:7AQC"
FT   STRAND          41..43
FT                   /evidence="ECO:0007829|PDB:6TNN"
FT   STRAND          45..47
FT                   /evidence="ECO:0007829|PDB:6PVK"
FT   STRAND          51..54
FT                   /evidence="ECO:0007829|PDB:7AQC"
FT   STRAND          61..63
FT                   /evidence="ECO:0007829|PDB:7AQC"
FT   STRAND          76..83
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          87..96
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   TURN            97..99
FT                   /evidence="ECO:0007829|PDB:6TNN"
FT   STRAND          101..105
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          107..109
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          115..119
FT                   /evidence="ECO:0007829|PDB:6PVK"
FT   STRAND          129..131
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   TURN            132..134
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          140..143
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          145..147
FT                   /evidence="ECO:0007829|PDB:6PPF"
FT   TURN            148..150
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          162..167
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          169..176
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   TURN            177..179
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          180..185
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          189..193
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   HELIX           198..202
FT                   /evidence="ECO:0007829|PDB:7AQC"
FT   HELIX           208..213
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   HELIX           222..224
FT                   /evidence="ECO:0007829|PDB:7AQC"
FT   TURN            227..229
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          236..238
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          243..245
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          249..251
FT                   /evidence="ECO:0007829|PDB:7AQC"
FT   STRAND          255..257
FT                   /evidence="ECO:0007829|PDB:7AS9"
FT   TURN            266..269
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          270..272
FT                   /evidence="ECO:0007829|PDB:7AQC"
SQ   SEQUENCE   277 AA;  30274 MW;  E126BCA251035A96 CRC64;
     MAIKKYKPTS NGRRGMTTSD FAEITTDKPE KSLLAPLHKK GGRNNQGKLT VRHQGGGHKR
     QYRVIDFKRD KDGIPGRVAT VEYDPNRSAN IALINYADGE KRYILAPKGI QVGTEIMSGP
     EADIKVGNAL PLINIPVGTV VHNIELKPGK GGQLVRSAGT SAQVLGKEGK YVLVRLNSGE
     VRMILSACRA SIGQVGNEQH ELINIGKAGR SRWKGIRPTV RGSVMNPNDH PHGGGEGRAP
     IGRKSPMSPW GKPTLGFKTR KKKNKSDKFI VRRRKNK
//
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