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Database: UniProt
Entry: P70974
LinkDB: P70974
Original site: P70974 
ID   RL13_BACSU              Reviewed;         145 AA.
AC   P70974;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   29-SEP-2021, entry version 133.
DE   RecName: Full=50S ribosomal protein L13 {ECO:0000255|HAMAP-Rule:MF_01366};
GN   Name=rplM {ECO:0000255|HAMAP-Rule:MF_01366}; OrderedLocusNames=BSU01490;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA   Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA   Kawamura F., Yoshikawa H., Takahashi H.;
RT   "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT   subtilis chromosome.";
RL   Microbiology 142:3039-3046(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 16.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   PROTEIN SEQUENCE OF 1-12, SUBUNIT, AND BINDING TO OBG.
RC   STRAIN=PY22;
RX   PubMed=10781545; DOI=10.1128/jb.182.10.2771-2777.2000;
RA   Scott J.M., Ju J., Mitchell T., Haldenwang W.G.;
RT   "The Bacillus subtilis GTP binding protein obg and regulators of the
RT   sigma(B) stress response transcription factor cofractionate with
RT   ribosomes.";
RL   J. Bacteriol. 182:2771-2777(2000).
RN   [5] {ECO:0007744|PDB:6HA1, ECO:0007744|PDB:6HA8}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.10 ANGSTROMS) OF 1-145 WITH AND WITHOUT
RP   VIRGINIAMYCIN M.
RX   PubMed=30126986; DOI=10.1073/pnas.1808535115;
RA   Crowe-McAuliffe C., Graf M., Huter P., Takada H., Abdelshahid M.,
RA   Novacek J., Murina V., Atkinson G.C., Hauryliuk V., Wilson D.N.;
RT   "Structural basis for antibiotic resistance mediated by the Bacillus
RT   subtilis ABCF ATPase VmlR.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:8978-8983(2018).
CC   -!- FUNCTION: This protein is one of the early assembly proteins of the 50S
CC       ribosomal subunit, although it is not seen to bind rRNA by itself. It
CC       is important during the early stages of 50S assembly.
CC       {ECO:0000255|HAMAP-Rule:MF_01366}.
CC   -!- SUBUNIT: Part of the 50S ribosomal subunit (PubMed:30126986). Binds to
CC       Obg (AC P20964) (PubMed:10781545). {ECO:0000269|PubMed:10781545,
CC       ECO:0000269|PubMed:30126986}.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uL13 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01366}.
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DR   EMBL; D64126; BAA10988.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11925.2; -; Genomic_DNA.
DR   PIR; G69695; G69695.
DR   RefSeq; NP_388030.2; NC_000964.3.
DR   RefSeq; WP_003241966.1; NZ_JNCM01000029.1.
DR   PDB; 3J3V; EM; 13.30 A; J=1-145.
DR   PDB; 3J3W; EM; 10.70 A; J=1-145.
DR   PDB; 3J9W; EM; 3.90 A; BM=1-145.
DR   PDB; 5NJT; EM; 3.80 A; c=4-145.
DR   PDB; 6HA1; EM; 3.10 A; J=1-145.
DR   PDB; 6HA8; EM; 3.50 A; J=1-145.
DR   PDB; 6HTQ; EM; 4.50 A; J=4-145.
DR   PDB; 6PPF; EM; 3.40 A; J=1-145.
DR   PDB; 6PPK; EM; 4.40 A; J=1-145.
DR   PDB; 6PVK; EM; 3.40 A; J=1-145.
DR   PDB; 6TNN; EM; 3.07 A; c=1-145.
DR   PDB; 6TPQ; EM; 3.07 A; c=1-145.
DR   PDB; 7AQC; EM; 2.99 A; J=1-145.
DR   PDB; 7AQD; EM; 3.10 A; J=1-145.
DR   PDB; 7AS8; EM; 2.90 A; N=1-145.
DR   PDB; 7AS9; EM; 3.50 A; N=1-145.
DR   PDBsum; 3J3V; -.
DR   PDBsum; 3J3W; -.
DR   PDBsum; 3J9W; -.
DR   PDBsum; 5NJT; -.
DR   PDBsum; 6HA1; -.
DR   PDBsum; 6HA8; -.
DR   PDBsum; 6HTQ; -.
DR   PDBsum; 6PPF; -.
DR   PDBsum; 6PPK; -.
DR   PDBsum; 6PVK; -.
DR   PDBsum; 6TNN; -.
DR   PDBsum; 6TPQ; -.
DR   PDBsum; 7AQC; -.
DR   PDBsum; 7AQD; -.
DR   PDBsum; 7AS8; -.
DR   PDBsum; 7AS9; -.
DR   SMR; P70974; -.
DR   STRING; 224308.BSU01490; -.
DR   jPOST; P70974; -.
DR   PaxDb; P70974; -.
DR   PRIDE; P70974; -.
DR   EnsemblBacteria; CAB11925; CAB11925; BSU_01490.
DR   GeneID; 50135109; -.
DR   GeneID; 938915; -.
DR   KEGG; bsu:BSU01490; -.
DR   PATRIC; fig|224308.179.peg.153; -.
DR   eggNOG; COG0102; Bacteria.
DR   InParanoid; P70974; -.
DR   OMA; DFVVIIN; -.
DR   PhylomeDB; P70974; -.
DR   BioCyc; BSUB:BSU01490-MONOMER; -.
DR   PRO; PR:P70974; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR   GO; GO:0005840; C:ribosome; IBA:GO_Central.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR   GO; GO:0017148; P:negative regulation of translation; IBA:GO_Central.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   CDD; cd00392; Ribosomal_L13; 1.
DR   Gene3D; 3.90.1180.10; -; 1.
DR   HAMAP; MF_01366; Ribosomal_L13; 1.
DR   InterPro; IPR005822; Ribosomal_L13.
DR   InterPro; IPR005823; Ribosomal_L13_bac-type.
DR   InterPro; IPR023563; Ribosomal_L13_CS.
DR   InterPro; IPR036899; Ribosomal_L13_sf.
DR   PANTHER; PTHR11545; PTHR11545; 1.
DR   Pfam; PF00572; Ribosomal_L13; 1.
DR   PIRSF; PIRSF002181; Ribosomal_L13; 1.
DR   SUPFAM; SSF52161; SSF52161; 1.
DR   TIGRFAMs; TIGR01066; rplM_bact; 1.
DR   PROSITE; PS00783; RIBOSOMAL_L13; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Reference proteome;
KW   Ribonucleoprotein; Ribosomal protein.
FT   CHAIN           1..145
FT                   /note="50S ribosomal protein L13"
FT                   /id="PRO_0000133725"
FT   CONFLICT        16
FT                   /note="W -> C (in Ref. 1; BAA10988)"
FT                   /evidence="ECO:0000305"
FT   STRAND          16..20
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          22..24
FT                   /evidence="ECO:0007829|PDB:7AQC"
FT   HELIX           26..38
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   TURN            39..41
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          42..44
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   TURN            47..49
FT                   /evidence="ECO:0007829|PDB:7AQC"
FT   STRAND          54..58
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   HELIX           60..62
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   HELIX           69..72
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          74..78
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          85..89
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   HELIX           90..96
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   HELIX           98..109
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   HELIX           114..120
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          123..125
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          127..129
FT                   /evidence="ECO:0007829|PDB:6TNN"
FT   HELIX           134..136
FT                   /evidence="ECO:0007829|PDB:7AQC"
SQ   SEQUENCE   145 AA;  16375 MW;  28563533DF244606 CRC64;
     MRTTPMANAS TIERKWLVVD AAGKTLGRLS SEVAAILRGK HKPTYTPHVD TGDHVIIINA
     EKIELTGKKL TDKIYYRHTQ HPGGLKSRTA LEMRTNYPEK MLELAIKGML PKGSLGRQMF
     KKLNVYRGSE HPHEAQKPEV YELRG
//
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