ID PG108_VAR67 Reviewed; 325 AA.
AC P33059;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 28-JAN-2026, entry version 79.
DE RecName: Full=Envelope protein H3;
DE AltName: Full=Ag35;
DE AltName: Full=Virion envelope protein p35;
GN Name=OPG108; ORFNames=H3L, I3L;
OS Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus;
OC Orthopoxvirus variola; Variola virus.
OX NCBI_TaxID=587200;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8383392; DOI=10.1016/0168-1702(93)90110-9;
RA Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S.,
RA Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V.,
RA Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G.,
RA Sandakhchiev L.S.;
RT "Nucleotide sequence analysis of variola virus HindIII M, L, I genome
RT fragments.";
RL Virus Res. 27:25-35(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT "Genes of variola and vaccinia viruses necessary to overcome the host
RT protective mechanisms.";
RL FEBS Lett. 319:80-83(1993).
CC -!- FUNCTION: Envelope protein that binds to heparan sulfate on the cell
CC surface and might provide virion attachment to target cell.
CC {ECO:0000250, ECO:0000250|UniProtKB:P07240}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:P07240};
CC Single-pass membrane protein {ECO:0000250|UniProtKB:P07240}.
CC Note=Component of the mature virion (MV) membrane. Becomes membrane
CC associated presumably during virus maturation. The mature virion is
CC located in the cytoplasm of infected cells and is probably released by
CC cell lysis. {ECO:0000250|UniProtKB:P07240}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC -!- PTM: Does not contain disulfide bonds. {ECO:0000250|UniProtKB:P07240}.
CC -!- MISCELLANEOUS: Immunodominant protein. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the orthopoxvirus OPG108 family. {ECO:0000305}.
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DR EMBL; X67119; CAA47585.1; -; Genomic_DNA.
DR EMBL; S55844; AAB24682.1; -; Genomic_DNA.
DR EMBL; X69198; CAA49027.1; -; Genomic_DNA.
DR PIR; S33100; S33100.
DR RefSeq; NP_042130.1; NC_001611.1.
DR SMR; P33059; -.
DR GeneID; 1486441; -.
DR KEGG; vg:1486441; -.
DR Proteomes; UP000002060; Segment.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046718; P:symbiont entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR InterPro; IPR004900; Poxvirus_P35.
DR Pfam; PF03213; Pox_P35; 1.
PE 2: Evidence at transcript level;
KW Host-virus interaction; Late protein; Membrane; Reference proteome;
KW Signal-anchor; Transmembrane; Transmembrane helix;
KW Viral attachment to host cell; Viral envelope protein; Virion;
KW Virus entry into host cell.
FT CHAIN 1..325
FT /note="Envelope protein H3"
FT /id="PRO_0000099209"
FT TOPO_DOM 1..285
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical; Signal-anchor"
FT /evidence="ECO:0000305"
FT TOPO_DOM 307..325
FT /note="Intravirion"
FT /evidence="ECO:0000255"
SQ SEQUENCE 325 AA; 37696 MW; EAB2FEE3CDEA6E40 CRC64;
MATVNKTPVI VVPVIDRPPS ETFPNLHEHI NDQKFDDVKD NEVMPEKRNV VIVKDDPDHY
KDYAFIHWTG GNIRNDDKYT HFFSGFCNTM CTEETKRNIA RHLALWDSKF FTELENKKVE
YVVIVENDNV IEDITFLRPV LKAMHDKKID ILQMREIITG NKVKTELVMD KNHVIFTYTG
GYDVSLSAYI IRVTTALNIV DEIIKSGGLS SGFYFEIARI ENEIKINRQI MDNSAKYVEH
DPRLVAEHRF ENMKPNFWSR IGTAAVKRYP GVMYAFTTPL ISFFGLFDIN VIGLIVILFI
MFMLIFNVKS KLLWFLTGTF VTAFI
//
