GenomeNet

Database: UniProt
Entry: Q4G1L2
LinkDB: Q4G1L2
Original site: Q4G1L2 
ID   Q4G1L2_EPTBU            Unreviewed;       273 AA.
AC   Q4G1L2;
DT   30-AUG-2005, integrated into UniProtKB/TrEMBL.
DT   30-AUG-2005, sequence version 1.
DT   29-SEP-2021, entry version 86.
DE   SubName: Full=Variable lymphocyte receptor B {ECO:0000313|EMBL:AAZ16361.1};
GN   Name=VLRB {ECO:0000313|EMBL:AAZ16361.1};
OS   Eptatretus burgeri (Inshore hagfish).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Cyclostomata; Myxini;
OC   Myxiniformes; Myxinidae; Eptatretinae; Eptatretus.
OX   NCBI_TaxID=7764 {ECO:0000313|EMBL:AAZ16361.1};
RN   [1] {ECO:0000313|EMBL:AAZ16361.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=15964979; DOI=10.1073/pnas.0503792102;
RA   Pancer Z., Saha N.R., Kasamatsu J., Suzuki T., Amemiya C.T., Kasahara M.,
RA   Cooper M.D.;
RT   "Variable lymphocyte receptors in hagfish.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:9224-9229(2005).
RN   [2] {ECO:0007829|PDB:2Z62, ECO:0007829|PDB:2Z63}
RP   X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 126-199 IN COMPLEX WITH
RP   N-ACETYL-D-GLUCOSAMINE, AND DISULFIDE BONDS.
RX   PubMed=17803912; DOI=10.1016/j.cell.2007.08.002;
RA   Kim H.M., Park B.S., Kim J.I., Kim S.E., Lee J., Oh S.C., Enkhbayar P.,
RA   Matsushima N., Lee H., Yoo O.J., Lee J.O.;
RT   "Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist
RT   Eritoran.";
RL   Cell 130:906-917(2007).
RN   [3] {ECO:0007829|PDB:2O6R}
RP   X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 24-200, AND DISULFIDE BONDS.
RX   PubMed=17192264; DOI=10.1074/jbc.M608471200;
RA   Kim H.M., Oh S.C., Lim K.J., Kasamatsu J., Heo J.Y., Park B.S., Lee H.,
RA   Yoo O.J., Kasahara M., Lee J.O.;
RT   "Structural diversity of the hagfish variable lymphocyte receptors.";
RL   J. Biol. Chem. 282:6726-6732(2007).
RN   [4] {ECO:0007829|PDB:3A79, ECO:0007829|PDB:3A7B}
RP   X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 133-200, AND DISULFIDE BONDS.
RX   PubMed=19931471; DOI=10.1016/j.immuni.2009.09.018;
RA   Kang J.Y., Nan X., Jin M.S., Youn S.J., Ryu Y.H., Mah S., Han S.H., Lee H.,
RA   Paik S.G., Lee J.O.;
RT   "Recognition of lipopeptide patterns by Toll-like receptor 2-Toll-like
RT   receptor 6 heterodimer.";
RL   Immunity 31:873-884(2009).
RN   [5] {ECO:0007829|PDB:3UL7, ECO:0007829|PDB:3UL8}
RP   X-RAY CRYSTALLOGRAPHY (2.37 ANGSTROMS) OF 126-199, AND DISULFIDE BONDS.
RX   PubMed=22363519; DOI=10.1371/journal.pone.0030929;
RA   Han J., Kim H.J., Lee S.C., Hong S., Park K., Jeon Y.H., Kim D.,
RA   Cheong H.K., Kim H.S.;
RT   "Structure-based rational design of a Toll-like receptor 4 (TLR4) decoy
RT   receptor with high binding affinity for a target protein.";
RL   PLoS ONE 7:E30929-E30929(2012).
RN   [6] {ECO:0007829|PDB:3V44, ECO:0007829|PDB:3V47}
RP   X-RAY CRYSTALLOGRAPHY (2.47 ANGSTROMS) OF 126-200, AND DISULFIDE BONDS.
RX   PubMed=22344444; DOI=10.1126/science.1215584;
RA   Yoon S.I., Kurnasov O., Natarajan V., Hong M., Gudkov A.V., Osterman A.L.,
RA   Wilson I.A.;
RT   "Structural basis of TLR5-flagellin recognition and signaling.";
RL   Science 335:859-864(2012).
RN   [7] {ECO:0007829|PDB:4ARR}
RP   X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 133-201, AND DISULFIDE BONDS.
RX   PubMed=23596340; DOI=10.1107/S0021889812051606;
RA   Gangloff M., Moreno A., Gay N.J.;
RT   "Liesegang-like patterns of Toll crystals grown in gel.";
RL   J. Appl. Crystallogr. 46:337-345(2013).
RN   [8] {ECO:0007829|PDB:4BV4}
RP   X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 133-201, AND DISULFIDE BONDS.
RX   PubMed=24282309; DOI=10.1073/PNAS.1317002110;
RA   Lewis M., Arnot C.J., Beeston H., McCoy A., Ashcroft A.E., Gay N.J.,
RA   Gangloff M.;
RT   "Cytokine Spatzle binds to the Drosophila immunoreceptor Toll with a
RT   neurotrophin-like specificity and couples receptor activation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:20461-20466(2013).
RN   [9] {ECO:0007829|PDB:4ARN}
RP   X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS) OF 133-201, AND DISULFIDE BONDS.
RX   PubMed=23245851; DOI=10.1016/j.str.2012.11.003;
RA   Gangloff M., Arnot C.J., Lewis M., Gay N.J.;
RT   "Functional insights from the crystal structure of the N-terminal domain of
RT   the prototypical toll receptor.";
RL   Structure 21:143-153(2013).
RN   [10] {ECO:0007829|PDB:4QDH}
RP   X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 21-81 AND 126-200, AND DISULFIDE
RP   BONDS.
RX   PubMed=24888966; DOI=10.1002/prot.24616;
RA   Collins B., Wilson I.A.;
RT   "Crystal structure of the C-terminal domain of mouse TLR9.";
RL   Proteins 82:2874-2878(2014).
RN   [11] {ECO:0007829|PDB:4QXF}
RP   X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 128-200, AND DISULFIDE BONDS.
RA   Xu J.G., Huang C., Zhou Y., Zhu Y.;
RT   "crystal structure of human LGR4 and Rspo1.";
RL   Submitted (JUL-2014) to the PDB data bank.
RN   [12] {ECO:0007829|PDB:4QXE}
RP   X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 131-200, AND DISULFIDE BONDS.
RA   Xu J.G., Huang C., Zhou Y., Zhu Y.;
RT   "Crystal structure of LGR4 fused with hagfish VLR.";
RL   Submitted (JUL-2014) to the PDB data bank.
RN   [13] {ECO:0007829|PDB:5IJB, ECO:0007829|PDB:5IJC}
RP   X-RAY CRYSTALLOGRAPHY (2.57 ANGSTROMS) OF 126-200, AND DISULFIDE BONDS.
RX   PubMed=26831104; DOI=10.1073/pnas.1525639113;
RA   Wang Y., Su L., Morin M.D., Jones B.T., Whitby L.R., Surakattula M.M.,
RA   Huang H., Shi H., Choi J.H., Wang K.W., Moresco E.M., Berger M., Zhan X.,
RA   Zhang H., Boger D.L., Beutler B.;
RT   "TLR4/MD-2 activation by a synthetic agonist with no similarity to LPS.";
RL   Proc. Natl. Acad. Sci. U.S.A. 113:E884-E893(2016).
RN   [14] {ECO:0007829|PDB:5GY2}
RP   X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 28-200, AND DISULFIDE BONDS.
RX   PubMed=28106112; DOI=10.1038/srep40878;
RA   Song W.S., Jeon Y.J., Namgung B., Hong M., Yoon S.I.;
RT   "A conserved TLR5 binding and activation hot spot on flagellin.";
RL   Sci. Rep. 7:40878-40878(2017).
RN   [15] {ECO:0007829|PDB:6BXA, ECO:0007829|PDB:6BXC}
RP   X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 126-200, AND DISULFIDE BONDS.
RX   PubMed=29596914; DOI=10.1016/j.jmb.2018.03.016;
RA   Gunn R.J., Herrin B.R., Acharya S., Cooper M.D., Wilson I.A.;
RT   "VLR Recognition of TLR5 Expands the Molecular Characterization of Protein
RT   Antigen Binding by Non-Ig-based Antibodies.";
RL   J. Mol. Biol. 430:1350-1367(2018).
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY965531; AAZ16361.1; -; mRNA.
DR   PDB; 2O6R; X-ray; 2.30 A; A=24-200.
DR   PDB; 2Z62; X-ray; 1.70 A; A=126-199.
DR   PDB; 2Z63; X-ray; 2.00 A; A=134-199.
DR   PDB; 2Z65; X-ray; 2.70 A; A/B=126-199.
DR   PDB; 2Z66; X-ray; 1.90 A; A/B/C/D=24-85.
DR   PDB; 3A79; X-ray; 2.90 A; A=133-200.
DR   PDB; 3A7B; X-ray; 2.53 A; A=133-200.
DR   PDB; 3A7C; X-ray; 2.40 A; A=133-200.
DR   PDB; 3UL7; X-ray; 2.37 A; A=126-200.
DR   PDB; 3UL8; X-ray; 2.50 A; A=126-199.
DR   PDB; 3UL9; X-ray; 2.45 A; A=126-199.
DR   PDB; 3ULA; X-ray; 3.60 A; A/C=126-199.
DR   PDB; 3V44; X-ray; 2.83 A; A=126-200.
DR   PDB; 3V47; X-ray; 2.47 A; A/B=126-200.
DR   PDB; 4ARN; X-ray; 2.41 A; A/B/C/D=136-201.
DR   PDB; 4ARR; X-ray; 3.00 A; A/B=136-201.
DR   PDB; 4BV4; X-ray; 2.35 A; R=134-201.
DR   PDB; 4QDH; X-ray; 2.40 A; A/B=126-200.
DR   PDB; 4QXE; X-ray; 2.20 A; A=134-200.
DR   PDB; 4QXF; X-ray; 2.25 A; A/B=129-200.
DR   PDB; 5GY2; X-ray; 2.10 A; A/B=126-200.
DR   PDB; 5IJB; X-ray; 2.91 A; A/B=126-200.
DR   PDB; 5IJC; X-ray; 2.57 A; A/B=126-200.
DR   PDB; 5IJD; X-ray; 2.70 A; A/B=126-200.
DR   PDB; 6BXA; X-ray; 2.30 A; A/B=126-200.
DR   PDB; 6BXC; X-ray; 2.50 A; A/B=126-200.
DR   PDBsum; 2O6R; -.
DR   PDBsum; 2Z62; -.
DR   PDBsum; 2Z63; -.
DR   PDBsum; 2Z65; -.
DR   PDBsum; 2Z66; -.
DR   PDBsum; 3A79; -.
DR   PDBsum; 3A7B; -.
DR   PDBsum; 3A7C; -.
DR   PDBsum; 3UL7; -.
DR   PDBsum; 3UL8; -.
DR   PDBsum; 3UL9; -.
DR   PDBsum; 3ULA; -.
DR   PDBsum; 3V44; -.
DR   PDBsum; 3V47; -.
DR   PDBsum; 4ARN; -.
DR   PDBsum; 4ARR; -.
DR   PDBsum; 4BV4; -.
DR   PDBsum; 4QDH; -.
DR   PDBsum; 4QXE; -.
DR   PDBsum; 4QXF; -.
DR   PDBsum; 5GY2; -.
DR   PDBsum; 5IJB; -.
DR   PDBsum; 5IJC; -.
DR   PDBsum; 5IJD; -.
DR   PDBsum; 6BXA; -.
DR   PDBsum; 6BXC; -.
DR   SMR; Q4G1L2; -.
DR   EvolutionaryTrace; Q4G1L2; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   Gene3D; 3.80.10.10; -; 2.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   InterPro; IPR000372; LRRNT.
DR   InterPro; IPR024592; Variable_lymphocyte_rcpt_C.
DR   Pfam; PF11921; DUF3439; 1.
DR   Pfam; PF13855; LRR_8; 1.
DR   Pfam; PF01462; LRRNT; 1.
DR   SMART; SM00369; LRR_TYP; 4.
DR   SMART; SM00013; LRRNT; 1.
DR   PROSITE; PS51450; LRR; 2.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:2O6R, ECO:0007829|PDB:2Z62};
KW   Leucine-rich repeat {ECO:0000256|ARBA:ARBA00022614};
KW   Membrane {ECO:0000256|SAM:Phobius}; Receptor {ECO:0000313|EMBL:AAZ16361.1};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737}; Signal {ECO:0000256|SAM:SignalP};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           24..273
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004238843"
FT   TRANSMEM        248..267
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          23..55
FT                   /note="LRRNT"
FT                   /evidence="ECO:0000259|SMART:SM00013"
FT   REGION          176..179
FT                   /note="Mannose binding"
FT                   /evidence="ECO:0007829|PDB:4ARN"
FT   REGION          190..191
FT                   /note="Beta-D-mannose binding"
FT                   /evidence="ECO:0007829|PDB:4ARN"
FT   BINDING         144
FT                   /note="Beta-D-mannose; via carbonyl oxygen"
FT                   /evidence="ECO:0007829|PDB:4ARN"
FT   BINDING         146
FT                   /note="Mannose; via carbonyl oxygen"
FT                   /evidence="ECO:0007829|PDB:4ARN"
FT   BINDING         153
FT                   /note="N-acetyl-D-glucosamine"
FT                   /evidence="ECO:0007829|PDB:4QDH, ECO:0007829|PDB:5IJD"
FT   BINDING         175
FT                   /note="Fucose"
FT                   /evidence="ECO:0007829|PDB:4ARN"
FT   BINDING         185
FT                   /note="Beta-D-mannose"
FT                   /evidence="ECO:0007829|PDB:4ARN"
FT   BINDING         188
FT                   /note="N-acetyl-D-glucosamine; via carbonyl oxygen"
FT                   /evidence="ECO:0007829|PDB:2Z62"
FT   DISULFID        24..30
FT                   /evidence="ECO:0007829|PDB:2O6R, ECO:0007829|PDB:2Z66"
FT   DISULFID        28..37
FT                   /evidence="ECO:0007829|PDB:2O6R, ECO:0007829|PDB:2Z66"
FT   DISULFID        161..186
FT                   /evidence="ECO:0007829|PDB:2O6R, ECO:0007829|PDB:2Z62"
FT   DISULFID        163..198
FT                   /evidence="ECO:0007829|PDB:2O6R, ECO:0007829|PDB:2Z62"
SQ   SEQUENCE   273 AA;  30001 MW;  5B0BBBB883D12E2B CRC64;
     MKFALRGTCV LLALLLCCRN GKACPSRCSC SGTEIRCNSK GLTSVPTGIP SSATRLELES
     NKLQSLPHGV FDKLTQLTKL SLSQNQIQSL PDGVFDKLTK LTILYLHENK LQSLPNGVFD
     KLTQLKELAL DTNQLKSVPD GIFDRLTSLQ KIWLHTNPWD CSCPRIDYLS RWLNKNSQKE
     QGSAKCSGSG KPVRSIICPT TTTTTTTTTM PTTTTLPTTT KMSMVKVPLV PPEAFGRVMN
     ACAYFPSYIF LHLVHGLAAV PLVYLICHAS QLL
//
DBGET integrated database retrieval system