GenomeNet

Database: UniProt
Entry: Q6CWJ2
LinkDB: Q6CWJ2
Original site: Q6CWJ2 
ID   Q6CWJ2_KLULA            Unreviewed;       261 AA.
AC   Q6CWJ2;
DT   16-AUG-2004, integrated into UniProtKB/TrEMBL.
DT   16-AUG-2004, sequence version 1.
DT   07-APR-2021, entry version 118.
DE   RecName: Full=40S ribosomal protein S4 {ECO:0000256|PIRNR:PIRNR002116};
GN   ORFNames=KLLA0_B03652g {ECO:0000313|EMBL:CAH02090.1};
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590 {ECO:0000313|Proteomes:UP000000598};
RN   [1] {ECO:0000313|EMBL:CAH02090.1, ECO:0000313|Proteomes:UP000000598}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 /
RC   WM37 {ECO:0000313|Proteomes:UP000000598};
RX   PubMed=15229592; DOI=10.1038/nature02579;
RG   Genolevures;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.F., Straub M.L.,
RA   Suleau A., Swennene D., Tekaia F., Wesolowski-Louvel M., Westhof E.,
RA   Wirth B., Zeniou-Meyer M., Zivanovic I., Bolotin-Fukuhara M., Thierry A.,
RA   Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J.,
RA   Wincker P., Souciet J.L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
RN   [2] {ECO:0007829|PDB:3J80, ECO:0007829|PDB:3J81}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.75 ANGSTROMS).
RX   PubMed=25417110; DOI=10.1016/j.cell.2014.10.001;
RA   Hussain T., Llacer J.L., Fernandez I.S., Munoz A., Martin-Marcos P.,
RA   Savva C.G., Lorsch J.R., Hinnebusch A.G., Ramakrishnan V.;
RT   "Structural changes enable start codon recognition by the eukaryotic
RT   translation initiation complex.";
RL   Cell 159:597-607(2014).
RN   [3] {ECO:0007829|PDB:3JAM, ECO:0007829|PDB:3JAP}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.46 ANGSTROMS).
RX   PubMed=26212456; DOI=10.1016/j.molcel.2015.06.033;
RA   Llacer J.L., Hussain T., Marler L., Aitken C.E., Thakur A., Lorsch J.R.,
RA   Hinnebusch A.G., Ramakrishnan V.;
RT   "Conformational differences between open and closed states of the
RT   eukaryotic translation initiation complex.";
RL   Mol. Cell 59:399-412(2015).
RN   [4] {ECO:0007829|PDB:5IT7, ECO:0007829|PDB:5IT9}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.60 ANGSTROMS) OF 2-261.
RX   PubMed=27159451; DOI=10.7554/eLife.13567;
RA   Murray J., Savva C.G., Shin B.S., Dever T.E., Ramakrishnan V.,
RA   Fernandez I.S.;
RT   "Structural characterization of ribosome recruitment and translocation by
RT   type IV IRES.";
RL   Elife 5:0-0(2016).
RN   [5] {ECO:0007829|PDB:6FYX, ECO:0007829|PDB:6FYY}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.05 ANGSTROMS).
RX   PubMed=30475211; DOI=10.7554/eLife.39273;
RA   Llacer J.L., Hussain T., Saini A.K., Nanda J.S., Kaur S., Gordiyenko Y.,
RA   Kumar R., Hinnebusch A.G., Lorsch J.R., Ramakrishnan V.;
RT   "Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal
RT   subunit to promote start-codon recognition.";
RL   Elife 7:0-0(2018).
RN   [6] {ECO:0007829|PDB:6GSM, ECO:0007829|PDB:6GSN}
RP   STRUCTURE BY ELECTRON MICROSCOPY (5.75 ANGSTROMS) OF 2-261.
RA   Llacer J.L., Hussain T., Gordiyenko Y., Ramakrishnan V.;
RT   "Towards a model of eIF3 on the 40S subunit interface in eukaryotic
RT   translation initiation.";
RL   Submitted (JUN-2018) to the PDB data bank.
RN   [7] {ECO:0007829|PDB:6UZ7}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.60 ANGSTROMS).
RX   PubMed=31900355; DOI=10.1073/pnas.1916436117;
RA   Huang B.Y., Fernandez I.S.;
RT   "Long-range interdomain communications in eIF5B regulate GTP hydrolysis and
RT   translation initiation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 117:1429-1437(2020).
CC   -!- SIMILARITY: Belongs to the eukaryotic ribosomal protein eS4 family.
CC       {ECO:0000256|ARBA:ARBA00007500, ECO:0000256|PIRNR:PIRNR002116}.
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DR   EMBL; CR382122; CAH02090.1; -; Genomic_DNA.
DR   RefSeq; XP_451697.1; XM_451697.1.
DR   PDB; 3J80; EM; 3.75 A; E=1-261.
DR   PDB; 3J81; EM; 4.00 A; E=1-261.
DR   PDB; 3JAM; EM; 3.46 A; E=1-261.
DR   PDB; 3JAP; EM; 4.90 A; E=1-261.
DR   PDB; 3JAQ; EM; 6.00 A; E=1-261.
DR   PDB; 5IT7; EM; 3.60 A; E=2-261.
DR   PDB; 5IT9; EM; 3.80 A; E=2-261.
DR   PDB; 6FYX; EM; 3.05 A; E=1-261.
DR   PDB; 6FYY; EM; 3.05 A; E=1-261.
DR   PDB; 6GSM; EM; 5.15 A; E=2-261.
DR   PDB; 6GSN; EM; 5.75 A; E=2-261.
DR   PDB; 6UZ7; EM; 3.60 A; E=1-261.
DR   PDBsum; 3J80; -.
DR   PDBsum; 3J81; -.
DR   PDBsum; 3JAM; -.
DR   PDBsum; 3JAP; -.
DR   PDBsum; 3JAQ; -.
DR   PDBsum; 5IT7; -.
DR   PDBsum; 5IT9; -.
DR   PDBsum; 6FYX; -.
DR   PDBsum; 6FYY; -.
DR   PDBsum; 6GSM; -.
DR   PDBsum; 6GSN; -.
DR   PDBsum; 6UZ7; -.
DR   SMR; Q6CWJ2; -.
DR   STRING; 28985.XP_451697.1; -.
DR   EnsemblFungi; CAH02090; CAH02090; KLLA0_B03652g.
DR   GeneID; 2897114; -.
DR   KEGG; kla:KLLA0_B03652g; -.
DR   eggNOG; KOG0378; Eukaryota.
DR   HOGENOM; CLU_060400_1_0_1; -.
DR   InParanoid; Q6CWJ2; -.
DR   OMA; FPVGLMD; -.
DR   Proteomes; UP000000598; Chromosome B.
DR   GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:UniProtKB-UniRule.
DR   GO; GO:0006412; P:translation; IEA:InterPro.
DR   CDD; cd06087; KOW_RPS4; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 2.30.30.30; -; 1.
DR   Gene3D; 2.40.50.740; -; 1.
DR   HAMAP; MF_00485; Ribosomal_S4e; 1.
DR   InterPro; IPR032277; 40S_S4_C.
DR   InterPro; IPR005824; KOW.
DR   InterPro; IPR041982; KOW_RPS4.
DR   InterPro; IPR014722; Rib_L2_dom2.
DR   InterPro; IPR000876; Ribosomal_S4e.
DR   InterPro; IPR013845; Ribosomal_S4e_central_region.
DR   InterPro; IPR038237; Ribosomal_S4e_central_sf.
DR   InterPro; IPR013843; Ribosomal_S4e_N.
DR   InterPro; IPR018199; Ribosomal_S4e_N_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   PANTHER; PTHR11581; PTHR11581; 2.
DR   Pfam; PF16121; 40S_S4_C; 1.
DR   Pfam; PF00467; KOW; 1.
DR   Pfam; PF00900; Ribosomal_S4e; 1.
DR   Pfam; PF08071; RS4NT; 1.
DR   Pfam; PF01479; S4; 1.
DR   PIRSF; PIRSF002116; Ribosomal_S4; 1.
DR   SMART; SM00363; S4; 1.
DR   PROSITE; PS00528; RIBOSOMAL_S4E; 1.
DR   PROSITE; PS50889; S4; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:3J80, ECO:0007829|PDB:3J81};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000598};
KW   Ribonucleoprotein {ECO:0000256|ARBA:ARBA00023274,
KW   ECO:0000256|PIRNR:PIRNR002116};
KW   Ribosomal protein {ECO:0000256|ARBA:ARBA00022980,
KW   ECO:0000256|PIRNR:PIRNR002116};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PIRNR:PIRNR002116};
KW   rRNA-binding {ECO:0000256|ARBA:ARBA00022730,
KW   ECO:0000256|PIRNR:PIRNR002116}.
FT   DOMAIN          42..105
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS50889"
SQ   SEQUENCE   261 AA;  29558 MW;  828C352CA9745E9F CRC64;
     MARGPKKHLK RLAAPHHWML DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE
     VKAILMQRHV KVDGKVRTDT TFPAGFMDVI TLEATNENFR LVYDVKGRFA VHRITDEEAS
     YKLAKVKKVQ LGKKGIPYVV THDGRTIRYP DPNIKVNDTV KVDLATGTIT DFIKFDTGKL
     VYVTGGRNLG RVGTIVHRER HEGGFDLVHI KDSLENTFVT RLNNVFVIGE PGRPWISLPK
     GKGIKLTISE ERDRRRAQHG L
//
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