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Database: UniProt
Entry: Q82X47
LinkDB: Q82X47
Original site: Q82X47 
ID   Q82X47_NITEU            Unreviewed;       425 AA.
AC   Q82X47;
DT   01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT   01-JUN-2003, sequence version 1.
DT   29-SEP-2021, entry version 118.
DE   SubName: Full=Ammonium transporter family {ECO:0000313|EMBL:CAD84359.1};
GN   Name=Rh50 {ECO:0000313|EMBL:CAD84359.1};
GN   OrderedLocusNames=NE0448 {ECO:0000313|EMBL:CAD84359.1};
OS   Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC
OS   14298).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=228410 {ECO:0000313|EMBL:CAD84359.1, ECO:0000313|Proteomes:UP000001416};
RN   [1] {ECO:0000313|EMBL:CAD84359.1, ECO:0000313|Proteomes:UP000001416}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298
RC   {ECO:0000313|Proteomes:UP000001416};
RX   PubMed=12700255; DOI=10.1128/JB.185.9.2759-2773.2003;
RA   Chain P., Lamerdin J., Larimer F., Regala W., Land M., Hauser L.,
RA   Hooper A., Klotz M., Norton J., Sayavedra-Soto L., Arciero D., Hommes N.,
RA   Whittaker M., Arp D.;
RT   "Complete genome sequence of the ammonia-oxidizing bacterium and obligate
RT   chemolithoautotroph Nitrosomonas europaea.";
RL   J. Bacteriol. 185:2759-2773(2003).
RN   [2] {ECO:0007829|PDB:3B9Y, ECO:0007829|PDB:3B9Z}
RP   X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 31-418.
RX   PubMed=18040042; DOI=10.1073/pnas.0709710104;
RA   Li X., Jayachandran S., Nguyen H.H., Chan M.K.;
RT   "Structure of the Nitrosomonas europaea Rh protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:19279-19284(2007).
RN   [3] {ECO:0007829|PDB:3B9W}
RP   X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) OF 25-425.
RX   PubMed=18032606; DOI=10.1073/pnas.0706563104;
RA   Lupo D., Li X.D., Durand A., Tomizaki T., Cherif-Zahar B., Matassi G.,
RA   Merrick M., Winkler F.K.;
RT   "The 1.3-A resolution structure of Nitrosomonas europaea Rh50 and
RT   mechanistic implications for NH3 transport by Rhesus family proteins.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:19303-19308(2007).
RN   [4] {ECO:0007829|PDB:3BHS}
RP   X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) OF 26-425.
RA   Gruswitz F., Ho C.-M., del Rosario M.C., Westhoff C.M., Stroud R.M.;
RT   "Nitrosomonas europaea Rh50 and mechanism of conduction by Rhesus protein
RT   family of channels.";
RL   Submitted (NOV-2007) to the PDB data bank.
CC   -!- INTERACTION:
CC       Q82X47; Q82X47: Rh50; NbExp=2; IntAct=EBI-15671610, EBI-15671610;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR   EMBL; AL954747; CAD84359.1; -; Genomic_DNA.
DR   RefSeq; WP_011111083.1; NC_004757.1.
DR   PDB; 3B9W; X-ray; 1.30 A; A=25-425.
DR   PDB; 3B9Y; X-ray; 1.85 A; A=31-418.
DR   PDB; 3B9Z; X-ray; 1.85 A; A=31-418.
DR   PDB; 3BHS; X-ray; 1.99 A; A=26-425.
DR   PDBsum; 3B9W; -.
DR   PDBsum; 3B9Y; -.
DR   PDBsum; 3B9Z; -.
DR   PDBsum; 3BHS; -.
DR   SMR; Q82X47; -.
DR   DIP; DIP-29966N; -.
DR   STRING; 228410.NE0448; -.
DR   TCDB; 1.A.11.4.4; the ammonium transporter channel (amt) family.
DR   EnsemblBacteria; CAD84359; CAD84359; NE0448.
DR   KEGG; neu:NE0448; -.
DR   eggNOG; COG0004; Bacteria.
DR   HOGENOM; CLU_021386_0_0_4; -.
DR   OMA; WGTLMQG; -.
DR   OrthoDB; 562287at2; -.
DR   PhylomeDB; Q82X47; -.
DR   EvolutionaryTrace; Q82X47; -.
DR   Proteomes; UP000001416; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0008519; F:ammonium transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   Gene3D; 1.10.3430.10; -; 1.
DR   InterPro; IPR029020; Ammonium/urea_transptr.
DR   InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR   InterPro; IPR002229; RhesusRHD.
DR   Pfam; PF00909; Ammonium_transp; 1.
DR   PRINTS; PR00342; RHESUSRHD.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:3B9W, ECO:0007829|PDB:3B9Y};
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001416};
KW   Signal {ECO:0000256|SAM:SignalP};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           27..425
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004297230"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        74..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        104..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        128..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        231..253
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        260..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        284..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        325..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        350..368
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          40..371
FT                   /note="Ammonium_transp"
FT                   /evidence="ECO:0000259|Pfam:PF00909"
FT   COILED          393..413
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   425 AA;  44618 MW;  8EBA1F0ED2D653C0 CRC64;
     MSKHLCFTAF SSIALFLLCF SSWASAVAPA EINEARLVAQ YNYSINILAM LLVGFGFLMV
     FVRRYGFSAT TGTYLVVATG LPLYILLRAN GIFGHALTPH SVDAVIYAEF AVATGLIAMG
     AVLGRLRVFQ YALLALFIVP VYLLNEWLVL DNASGLTEGF QDSAGSIAIH AFGAYFGLGV
     SIALTTAAQR AQPIESDATS DRFSMLGSMV LWLFWPSFAT AIVPFEQMPQ TIVNTLLALC
     GATLATYFLS ALFHKGKASI VDMANAALAG GVAIGSVCNI VGPVGAFVIG LLGGAISVVG
     FVFIQPMLES KAKTIDTCGV HNLHGLPGLL GGFSAILIVP GIAVAQLTGI GITLALALIG
     GVIAGALIKL TGTTKQAYED SHEFIHLAGP EDEHKAERLV LEAKTEIQGL KNRIDAAVLS
     AKSEG
//
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